Neurochemical Journal

, Volume 13, Issue 2, pp 188–194 | Cite as

Collapsin Response Mediator Protein 2, a Potential Therapeutic Target in Temporal Lobe Epilepsy

  • Xue Wang
  • Wuqiong Zhang
  • Jiaai Li
  • Miaomiao Yu
  • Ming DongEmail author
  • Hongmei MengEmail author
Experimental Articles


Temporal lobe epilepsy (TLE) is the most prevalent subtype of epilepsy in humans and can easily develop into refractory epilepsy. The development of TLE is a complex process involving acute insults, a latent period, and chronic recurrent seizures. The latent period could provide an opportunity for intervention if molecular targets were to be identified. Collapsin response mediator protein 2 (CRMP2) was recently found to be involved in the mechanism of epileptogenesis. However, the results were inconsistent. We aimed to determine if CRMP2 is differentially expressed in rats following status epilepticus (SE), and to validate the expression of CRMP2 during the latent period. We used kainic acid (KA) as the initial insult to induce SE in rats and applied two-dimensional gel electrophoresis (2D-DIGE) coupled with mass spectrometry (MALDI-TOF/TOF) to search for aberrant protein expression in the hippocampus. Western blots were used to confirm the expression level of proteins. Protein extracts of the hippocampus from the sham and the KA groups were separated by 2D-DIGE; 17 protein spots on the gel, representing 10 unique proteins, were found to be significantly different between the KA and sham groups. Among those, CRMP2 was significantly up-regulated. Results of western blot analysis confirmed the increased CRMP2 levels in the KA group. Our findings indicate that the increase in CRMP2 during the latent period is possibly involved in the mechanism of epileptogenesis and is expected to be a novel therapeutic target.


temporal lobe epilepsy CRMP2 hippocampus proteomics 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.



We would like to thank Editage for English language editing.


  1. 1.
    Javidan, M., Epilepsy Res. Treat., 2012, vol. 2012, pp. 637430.Google Scholar
  2. 2.
    Graebenitz, S., Kedo, O., Speckmann, E.J., Gorji, A., Panneck, H., Hans, V., Palomero-Gallagher, N., Schleicher, A., Zilles, K., and Pape, H.C., Brain, 2011, vol. 134, pp. 2929–2947.CrossRefGoogle Scholar
  3. 3.
    Strzelczyk, A., Griebel, C., Lux, W., Rosenow, F., and Reese, J.P., Front Neurol., 2017, vol. 8, pp. 712.CrossRefGoogle Scholar
  4. 4.
    Yu, Y.H., Lee, K., Sin, D.S., Park, K.H., Park, D.K., and Kim, D.S., Brain Res. Bull., 2017, vol. 131, pp. 25–38.CrossRefGoogle Scholar
  5. 5.
    Gorter, J.A., van Vliet, E.A., and Aronica, E., Epilepsy Behav., 2015, vol. 49, pp. 13–16.CrossRefGoogle Scholar
  6. 6.
    Stone, T.J., Rowell, R., Jayasekera, B.A.P., Cunningham, M.O., and Jacques, T.S., Neuropathol. Appl. Neurobiol., 2018, vol. 44, pp. 56–69.CrossRefGoogle Scholar
  7. 7.
    Durand, E., Watier, L., Fix, M., Weiss, J.J., Chevignard, M., and Pradat-Diehl, P., Brain Inj., 2016, vol. 30, pp. 363–372.CrossRefGoogle Scholar
  8. 8.
    Goshima, Y., Nakamura, F., Strittmatter, P., and Strittmatter, S.M., Nature, 1995, vol. 376, pp. 509–514.CrossRefGoogle Scholar
  9. 9.
    Khanna, R., Wilson, S.M., Brittain, J.M., Weimer, J., Sultana, R., Butterfield, A., and Hensley, K., Future Neurol., 2012, vol. 7, pp. 749–771.CrossRefGoogle Scholar
  10. 10.
    Czech, T., Yang, J.W., Csaszar, E., Kappler, J., Baumgartner, C., and Lubec, G., Neurochem. Res., 2004, vol. 29, pp. 2189–2196.CrossRefGoogle Scholar
  11. 11.
    Lee, C.Y., Jaw, T., Tseng, H.C., Chen, I.C., and Liou, H.H., PLoS One, 2012, vol. 7, p. e38789.CrossRefGoogle Scholar
  12. 12.
    Racine, R.J., Electroencephalogr. Clin. Neurophysiol, 1972, vol. 32, pp. 281–294.CrossRefGoogle Scholar
  13. 13.
    Lubin, F.D., Johnston, L.D., Sweatt, J.D., and Anderson, A.E., Neuroscience, 2005, vol. 133, pp. 969–981.CrossRefGoogle Scholar
  14. 14.
    Wong, M., Epilepsy Curr., 2009, vol. 9, pp. 144–145.CrossRefGoogle Scholar
  15. 15.
    Morinaka, A., Yamada, M., Itofusa, R., Funato, Y., Yoshimura, Y., Nakamura, F., Yoshimura, T., Kaibuchi, K., Goshima, Y., Hoshino, M., Kamiguchi, H., and Miki, H., Sci. Signal, 2011, vol. 4, p. ra26.CrossRefGoogle Scholar
  16. 16.
    Inagaki, N., Chihara, K., Arimura, N., Menager, C., Kawano, Y., Matsuo, N., Nishimura, T., Amano, M., and Kaibuchi, K., Nat. Neurosci., 2001, vol. 4, pp. 781–782.CrossRefGoogle Scholar
  17. 17.
    Greene, N.D., Bamidele, A., Choy, M., de Castro, S.C., Wait, R., Leung, K.Y., Begum, S., Gadian, D.G., Scott, R.C., and Lythgoe, M.F., Proteomics, 2007, vol. 7, pp. 1336–1344.CrossRefGoogle Scholar
  18. 18.
    Wilson, S.M., Xiong, W., Wang, Y., Ping, X., Head, J.D., Brittain, J.M., Gagare, P.D., Ramachandran, P.V., Jin, X., and Khanna, R., Neuroscience, 2012, vol. 210, pp. 451–466.CrossRefGoogle Scholar
  19. 19.
    Zhang, Z., Ottens, A.K., Sadasivan, S., Kobeissy, F.H., Fang, T., Hayes, R.L., and Wang, K.K., J. Neurotrauma, 2007, vol. 24, pp. 460–472.CrossRefGoogle Scholar
  20. 20.
    Arimura, N., Menager, C., Kawano, Y., Yoshimura, T., Kawabata, S., Hattori, A., Fukata, Y., Amano, M., Goshima, Y., Inagaki, M., Morone, N., Usukura, J., and Kaibuchi, K., Mol. Cell Biol., 2005, vol. 25, pp. 9973–9984.CrossRefGoogle Scholar
  21. 21.
    Brown, M., Jacobs, T., Eickholt, B., Ferrari, G., Teo, M., Monfries, C., Qi, R.Z., Leung, T., Lim, L., and Hall, C., J. Neurosci., 2004, vol. 24, pp. 8994–9004.CrossRefGoogle Scholar
  22. 22.
    Uchida, Y., Ohshima, T., Yamashita, N., Ogawara, M., Sasaki, Y., Nakamura, F., and Goshima, Y., J. Biol. Chem., 2009, vol. 284, pp. 27393–27401.CrossRefGoogle Scholar
  23. 23.
    Hou, S.T., Jiang, S.X., Aylsworth, A., Ferguson, G., Slinn, J., Hu, H., Leung, T., Kappler, J., and Kaibuchi, K., J. Neurochem., 2009, vol. 111, pp. 870–881.CrossRefGoogle Scholar
  24. 24.
    Astle, M.V., Ooms, L.M., Cole, A.R., Binge, L.C., Dyson, J.M., Layton, M.J., Petratos, S., Sutherland, C., and Mitchell, C.A., J. Biol. Chem., 2011, vol. 286, pp. 23407–23418.CrossRefGoogle Scholar
  25. 25.
    Zhu, L.Q., Zheng, H.Y., Peng, C.X., Liu, D., Li, H.L., Wang, Q., and Wang, J.Z., J. Neurosci., 2010, vol. 30, pp. 3839–3848.CrossRefGoogle Scholar
  26. 26.
    Wilson, S.M., Ki Yeon, S., Yang, X.F., Park, K.D., and Khanna, R., Front. Cell Neurosci., 2014, vol. 8, pp. 135.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  1. 1.Department of Neurology and Neuroscience centerFirst Hospital of Jilin UniversityChangchun, JilinPeople’s Republic of China

Personalised recommendations