Doklady Biochemistry and Biophysics

, Volume 485, Issue 1, pp 101–103 | Cite as

Optimization of Heterologous Expression of Insulin Receptor-Related Receptor Ectodomain

  • A. A. MozhaevEmail author
  • A. N. Orsa
  • I. E. Deyev
  • V. I. Shvets
  • A. G. Petrenko


In this paper, we present an approach to optimize the heterologous expression of the receptor tyrosine kinase IRR, which further simplifies the purification of the IRR from the medium and increases the final yield. The approach proposed by us can find application in the biotechnological production of other large-scale recombinant proteins produced for medical purposes.



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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • A. A. Mozhaev
    • 1
    • 2
    Email author
  • A. N. Orsa
    • 1
  • I. E. Deyev
    • 1
  • V. I. Shvets
    • 3
  • A. G. Petrenko
    • 1
  1. 1.Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesMoscowRussia
  2. 2.Shubnikov Institute of Crystallography, Crystallography and Photonics Federal Research Center, Russian Academy of SciencesMoscowRussia
  3. 3.Institute of Fine Chemical Technology, Russian Technological University (MIREA)MoscowRussia

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