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Doklady Biochemistry and Biophysics

, Volume 483, Issue 1, pp 337–340 | Cite as

Effect of Dinitrosyl Iron Complexes (NO Donors) on the Metabolic Processes in Human Fibroblasts

  • A. R. Gizatullin
  • N. P. Akentieva
  • N. A. Sanina
  • N. Yu. Shmatko
  • N. S. Goryachev
  • N. I. Shkondina
  • T. R. Prichodchenko
  • N. Zhelev
  • S. M. Aldoshin
Biochemistry, Biophysics, and Molecular Biology
  • 13 Downloads

Abstract

The results of the study of the effect of mononuclear dinitrosyl iron complexes (DNICs) with functional sulfur-containing ligands (NO donors) on the cell viability and metabolism of human lung fibroblasts are presented, and the efficiency of their action is evaluated. It was shown that cationic DNICs increased the cell viability of fibroblasts and demonstrated the cytoprotective properties. Fluorescent analysis revealed that the DNICs compounds decrease the mitochondrial membrane potential but do not have a significant effect on the level of glutathione and reactive oxygen species in fibroblasts. It is assumed that the DNICs have the therapeutic potential for treating cardiovascular diseases.

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References

  1. 1.
    Murad, F., Biosci. Rep., 1999, vol. 19, pp. 133–154.CrossRefGoogle Scholar
  2. 2.
    Drexler, H., Cardiovasc. Res., 1999, vol. 43, pp. 572–579.CrossRefGoogle Scholar
  3. 3.
    Wink, D.A. and Mitchell, J.B., Free Radic. Biol. Med., 1998, vol. 25, pp. 434–456.CrossRefGoogle Scholar
  4. 4.
    Davis, K.L., Martin, E., Turko, I.V., and Murad, F., Annu. Rev. Pharmacol. Toxicol., 2001, vol. 41, pp. 203–236.CrossRefGoogle Scholar
  5. 5.
    Miyamoto, Y., Saito, Y., Kajiyama, N., Yoshimura, M., Shimasaki, Y., Nakayama, M., Kamitani, S., Harada, M., Ishikawa, M., Kuwahara, K., Ogawa, E., Hamanaka, I., Takahashi, N., Kaneshige, T., Teraoka, H., Akamizu, T., Azuma, N., Yoshimasa, Y., Yoshimasa, T., Itoh, H., Masuda, I., Yasue, H., and Nakao, K., Hypertension, 1998, vol. 32, pp. 3–8.CrossRefGoogle Scholar
  6. 6.
    Lewandowska, H., Kalinowska, M., Brzoska, K., Wojciuk, K., Wojciuk, G., and Kruszewski, M., Dalton. Trans., 2011, vol. 40, pp. 8273–8289.CrossRefGoogle Scholar
  7. 7.
    Kapelko, V.I., Lakomkin, V.L., Abramov, A.A., Lukoshkova, E.V., Undrovinas, N.A., Khapchaev, A.Y., and Shirinsky, V.P., Oxid. Med. Cell. Longevity, 2017, vol. 2017, pp. 1–10.CrossRefGoogle Scholar
  8. 8.
    Sanina, N.A., Aldoshin, S.M., Shmatko, N.Yu., Korchagin, D.V., Shilov, G.V., Knyaz’kina, E.A., Ovanesyan, N.S., and Kulikov, A.V., New J. Chem., 2015, vol. 39, pp. 1022–1030.CrossRefGoogle Scholar
  9. 9.
    Schreer, A., Tinson, C., Sherry, J.P., and Schirmer, K., Anal. Biochem., 2005, vol. 344, pp. 76–85.CrossRefGoogle Scholar
  10. 10.
    Hissin, P.J. and Hilf, R., Anal. Biochem., 1976, vol. 74, pp. 214–226.CrossRefGoogle Scholar
  11. 11.
    Oksvold, M.P., Skarpen, E., Widerberg, J., and Huitfeldt, H.S., J. Histochem. Cytochem., 2002, vol. 50, pp. 289–303.CrossRefGoogle Scholar
  12. 12.
    Lisa, F.Di., Blank, P.S., Colonna, R., Gambassi, G., Silverman, H.S., Stern, M.D., and Hansford, R.G., J. Physiol., 1995, vol. 486, pp. 1–13.CrossRefGoogle Scholar
  13. 13.
    Efremov, R.G., Baradaran, R., and Sazanov, L.A., Nature, 2010, vol. 465, pp. 441–445.CrossRefGoogle Scholar
  14. 14.
    Joshi, D.C. and Bakowska, J.C., J. Vis. Exp., 2011, vol. 51, pp. 1–4.Google Scholar
  15. 15.
    Strużńka, L., Chalimoniuk, M., and Sulkowski, G., Toxicology, 2005, vol. 212, pp. 185–194.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • A. R. Gizatullin
    • 1
  • N. P. Akentieva
    • 1
  • N. A. Sanina
    • 1
    • 2
  • N. Yu. Shmatko
    • 1
  • N. S. Goryachev
    • 1
    • 2
  • N. I. Shkondina
    • 1
  • T. R. Prichodchenko
    • 1
  • N. Zhelev
    • 3
  • S. M. Aldoshin
    • 1
    • 2
  1. 1.Institute of Problems of Chemical PhysicsRussian Academy of SciencesChernogolovka, Moscow oblastRussia
  2. 2.Moscow State UniversityMoscowRussia
  3. 3.Abertay UniversityDundeeUK

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