Synthesis of the Tripeptides Tyr-Thr-Lys Phosphorylated with Isopropyl Methyl- and (Deuteromethyl)phosphonochloridates as Reference Standards for the Analysis of Biomedical Samples
- 7 Downloads
A procedure for the phosphorylation of the tripeptide Tyr-Thr-Lys with isopropyl methyl- or (deuteromethyl)phosphonochloridate is developed. The phosphorylated tripeptides are intended for use as reference standards in the analysis of blood samples of people suspected to have been exposed to acetylcholinesterase inhibitors. Conditions of hromatographic separation and purification of the synthesized compounds are determined and optimized, which ensures the preparation of high-purity phosphorylated tripeptides.
Keywordsbiomarker tripeptide phosphorylation NMR spectroscopy
Unable to display preview. Download preview PDF.
This work was supported by the Russian Science Foundation (grant no. 19-13-00057) for Lomonosov Moscow State University.
Conflict of Interest
No conflict of interest was declared by the authors.
- 1.Convention on the Prohibition of the Development, Production, Stockpiling and Use of Chemical Weapons and on their Destruction. https://www.opcw.org/chemical-weapons-convention.
- 4.Sato, A., Yoshida, M., and Hara, S., Chem. Commun., 2008, no. 46, p. 6242. https://doi.org/10.1039/B814804J
- 5.Gershkovich, A.A. and Kibirev, V.K., Sintez peptidov. Reagenty i metody (Synthesis of Peptides. Reagents and Methods), Kiev: Naukova Dumka, 1987.Google Scholar