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Russian Journal of General Chemistry

, Volume 89, Issue 10, pp 2103–2107 | Cite as

Synthesis of the Tripeptides Tyr-Thr-Lys Phosphorylated with Isopropyl Methyl- and (Deuteromethyl)phosphonochloridates as Reference Standards for the Analysis of Biomedical Samples

  • I. A. RodinEmail author
  • T. M. Baygildiev
  • V. I. Krylov
  • V. N. Osipov
  • I. I. Krylov
  • V. A. Yashkir
  • I. V. Rybalchenko
Article
  • 7 Downloads

Abstract

A procedure for the phosphorylation of the tripeptide Tyr-Thr-Lys with isopropyl methyl- or (deuteromethyl)phosphonochloridate is developed. The phosphorylated tripeptides are intended for use as reference standards in the analysis of blood samples of people suspected to have been exposed to acetylcholinesterase inhibitors. Conditions of hromatographic separation and purification of the synthesized compounds are determined and optimized, which ensures the preparation of high-purity phosphorylated tripeptides.

Keywords

biomarker tripeptide phosphorylation NMR spectroscopy 

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Notes

Funding

This work was supported by the Russian Science Foundation (grant no. 19-13-00057) for Lomonosov Moscow State University.

Conflict of Interest

No conflict of interest was declared by the authors.

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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • I. A. Rodin
    • 1
    Email author
  • T. M. Baygildiev
    • 1
  • V. I. Krylov
    • 3
  • V. N. Osipov
    • 2
  • I. I. Krylov
    • 3
  • V. A. Yashkir
    • 3
  • I. V. Rybalchenko
    • 3
  1. 1.Chemical DepartmentLomonosov Moscow State UniversityMoscowRussia
  2. 2.Farmsintez ZAOMoscowRussia
  3. 3.27 Research CenterMinistry of Defense of the Russian FederationMoscowRussia

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