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Russian Journal of General Chemistry

, Volume 88, Issue 13, pp 2898–2902 | Cite as

Study of Antioxidant Activity and Composition of Cyanobacteria Metabolites by TLC, HPTLC, and HPLC for the Search of Environmentally Safe Cleaning Agents

  • Yu. V. BataevaEmail author
  • M. S. Satkalieva
  • S. V. Antonova
  • M. A. Sinetova
  • A. Yu. Kozlova
  • O. V. Astafyeva
  • A. S. Baimuhambetova
Article
  • 3 Downloads

Abstract

The composition of metabolites of cyanobacteria cultures of the Astrakhan region has been studied by thin-layer chromatography, high-performance thin layer chromatography, and high-performance liquid chromatography. Thin layer chromatography analysis of the cyanobacteria metabolites has shown the best separation and quality of chromatographic zones in seven systems: chloroform, ethanol: acetic acid (1: 1), butanol, ethanol: water (1: 9), butanol: acetic acid: water (4: 1: 5), ethanol: water (9: 1), and butanol: acetic acid: water (4: 1: 5). The flavonoid quercetin has been detected in an aqueous–alcoholic extract by high -performance thin-layer chromatography. The high-performance liquid chromatography has allowed identification of aspartic, isomonic, lactic, and fumaric acids in the cyanobacteria metabolites. Antioxidant activity of aqueous and aqueous-alcoholic extracts of the Phormidium ramosum B.-Peters IPPAS B-2022 culture has been detected.

Keywords

cyanobacteria metabolites thin-layer chromatography liquid chromatography antioxidant activity 

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Copyright information

© Pleiades Publishing, Ltd 2019

Authors and Affiliations

  • Yu. V. Bataeva
    • 1
    Email author
  • M. S. Satkalieva
    • 1
  • S. V. Antonova
    • 2
  • M. A. Sinetova
    • 3
  • A. Yu. Kozlova
    • 3
  • O. V. Astafyeva
    • 1
  • A. S. Baimuhambetova
    • 1
  1. 1.Astrakhan State UniversityAstrakhanRussia
  2. 2.National Research Center “Kurchatov Institute,”MoscowRussia
  3. 3.K.A. Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia

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