A New Approach for the Hybridization Analysis of Pathogens
The paper proposes a new variant for registering pathogens in the many-probes one-spot hybridization system. Using the example of Chlamydia trachomatis detection in blood serum samples, it has been demonstrated that hybridization of the analyzed DNA is characterized by a considerably stronger signal and higher specificity which may be advantageous for PointOfCare diagnostics (POC diagnostics).
Keywords:Chlamydia trachomatis oligonucleotide microarrays biosensor POC diagnostics
We are grateful to M.K. Ivanov (AO Vector-Best, Novosibirsk, Russia) for facilitating this research work.
The work was supported by the Basic Research Program of the State Academies of Sciences for years 2017–2020 (grant no. VI.62.1.4, 0309-2016-0004).
COMPLIANCE WITH ETHICAL STANDARDS
Conflict of Interests
Authors declare no conflict of interest.
Statement of Compliance with Standards of Research Involving Humans as Subjects
All procedures performed in the studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.