Russian Journal of Ecology

, Volume 50, Issue 3, pp 289–299 | Cite as

Effects of Rapid Enclosure of Aquatic Ecosystems on Genetic Diversity and Genetic Structure of Daphnia similoides sinensis in a Eutrophic Chinese Lake

  • Jianxun Wu
  • Kun Zhang
  • Daogui DengEmail author
  • Qi Liu
  • Shuixiu Peng
  • Tingting Zhang
  • Zhongze ZhouEmail author


The transformation of ecosystems influences the genetic diversity and evolution of aquatic organisms. However, the mechanisms by which the genetic structure of the Daphnia population responds to the changes in ecosystem remain unclear. We used the mitochondrial cytochrome oxidase subunit (CO1) and 12S genes and microsatellite markers to investigate the genetic structure and differentiation of the Daphnia similoides sinensis population in the sediments (core depth, 25 cm) of Lake Junshan, China. The construction of a lake embankment in 1958 changed the lake ecosystem from an open to a closed state, interrupting species exchange and thus leading to a continuous decrease in the genetic diversity of the D. similoides sinensis population during 1959–2010 (14-3 cm), although its genetic structure was stable based on the analysis of 14 microsatellite markers. However, after 2010, the genetic diversity indexes showed an increase in the genetic diversity of D. similoides sinensis; there was also a significant change in the genetic structure. The changes in the genetic diversity and structure of D. similoides sinensis significantly correlated with eutrophication. The results suggest that lake embankment can reduce the genetic diversity of D. similoides sinensis during the early stages. The D. similoides sinensis population accelerated genetic differentiation during the rapid lake eutrophication period due to continuing barrier effect. This study clearly reveals the effects mechanisms of the rapid enclosure of aquatic ecosystems on the species diversity and genetic differentiation of aquatic organisms.


Daphnia similoides sinensis ecosystem change genetic differentiation lake eutrophication resting egg sediment 


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  1. 1.
    Scheffer, M., Carpenter, S., Foley, J.A., Folke, C., and Walker, B., Catastrophic shifts in ecosystems, Nature, 2001, vol. 413, pp. 591–596.Google Scholar
  2. 2.
    Morita, K. and Yamamoto, S., Effects of habitat fragmentation by damming on the persistence of stream-dwelling charr populations, Conserv. Biol., 2002, vol. 16, no. 5, pp. 1318–1323.Google Scholar
  3. 3.
    Yi, Y., Yang, Z., and Zhang, S., Ecological influence of dam construction and river-lake connectivity on migration fish habitat in the Yangtze River basin, China, Proc. Environ. Sci., 2010, vol. 2, no. 5, pp. 1942–1954.Google Scholar
  4. 4.
    Naiman, R.J., Melillo, J.M., Lock, M.A., Ford, T.E., and Reice, S.R., Longitudinal patterns of ecosystem processes and community structure in a subarctic river continuum, Ecology, 1987, vol. 68, no. 5, pp. 1139–1156.Google Scholar
  5. 5.
    Wang, L.M., Hu, H.J., and Wang, D., Ecological impacts of disconnection from the Yangtze on fish resources in Zhangdu Lake, Resour. Environ. Yangtze Basin, 2005, vol. 14, no. 3, pp. 287–292.Google Scholar
  6. 6.
    Pang, M.X., Yu, X.M., and Tong, J.G., The microsatellite analysis of genetic diversity of five silver carp populations in the three gorges reservoir of the Yangtze River, Acta Hydrobiol. Sinica, 2015, vol. 39, no. 5, pp. 869–876.Google Scholar
  7. 7.
    Li, F., Study on genetic diversity of longsnout catfish (Letocasis longirostris Gunther) in and out of the Three Gorges Reservoir by the control region of mitochon-drial DNA, Ph.D. Thesis, Southwest Univ., 2007.Google Scholar
  8. 8.
    Li, K., Zhu, C., Wu, L., and Huang, L., Problems caused by the Three Gorges Dam construction in the Yangtze River basin: A review, Environ. Rev., 2013, vol.21, no. 3, pp. 127–135.Google Scholar
  9. 9.
    Yao, S.C. and Shen, J., A preliminary study of N-alkanes in a sedimentary core from Chaohu Lake, J. Lake Sci., 2003, vol. 15, no. 3, pp. 200–204.Google Scholar
  10. 10.
    Li, J., Liu, C.Q., Wang, S.L., Zhou, Z.H., Zhu, Z.Z., and Xiao, H.Y., Seasonal variations in composition and distribution of dissolved nutrients in the water column of Taihu Lake, China, Geol. Geochem., 2005, vol. 33, no. 1, pp. 63–67.Google Scholar
  11. 11.
    Korhola, A. and Rautio, M., Cladocera and Other Branchiopod Crustaceans, Springer Netherlands, 2001, vol. 4, pp. 5–41.Google Scholar
  12. 12.
    Thomsen, P.F. and Willerslev, E., Environmental DNA: An emerging tool in conservation for monitoring past and present biodiversity, Biol. Conserv., 2015, vol. 183, pp. 4–18.Google Scholar
  13. 13.
    Huang, Q., Xu, S.L., Xu, L., and Han, B.P., Haplotype diversity and genetic differentiation of dormant and active populations of Daphnia galeata in Liuxihe Reservoir of Guangdong Province, southern China, J. Lake Sci., 2017, vol. 29, no. 5, pp. 1209–1216.Google Scholar
  14. 14.
    Orsini, L., Schwenk, K., De Meester, L., Colbourne, J.K., Pfrender, M.E., and Weider, L.J., The evolutionary time machine: Using dormant propagules to forecast how populations can adapt to changing environments, Trends Ecol. Evol., 2013, vol. 28, pp. 274–282.Google Scholar
  15. 15.
    Hairston, N. and De Meester, L., Daphnia paleogenetics and environmental change: Deconstructing the evolution of plasticity, Int. Rev. Hydrobiol., 2008, vol. 93, no. 4, pp. 578–592.Google Scholar
  16. 16.
    Domaizon, I., Savichtcheva, O., Debroas, D., and Arnaud, F., DNA from lake sediments reveals the long-term dynamics and diversity of Synechococcus assemblages, Biogeosciences, 2013, vol. 10, pp. 2515–2564.Google Scholar
  17. 17.
    Hou, W., Dong, H., Li, G., Yang, J., Coolen, M.J., Liu, X., Wang, S., Jiang, H., Wu, X., Xiao, H., Lian, B., and Wan, Y., Identification of photosynthetic plankton communities using sedimentary ancient DNA and their response to late-Holocene climate change on the Tibetan Plateau, Sci. Rep., 2014, vol. 4, Article no. 6648.Google Scholar
  18. 18.
    Black, A.R., Predator-induced phenotypic plasticity in Daphnia pulex: Life history and morphological responses to Notonecta and Chaoborus, Limnol. Oceanogr., 1993, vol. 38, pp. 986–996.Google Scholar
  19. 19.
    Dodson, S.I. and Hanazato, T., Commentary on effects of anthropogenic and natural organic chemicals on development, swimming behavior, and reproduction of Daphnia, a key member of aquatic ecosystem, Environ. Health Perspect, 1995, vol. 103, pp. 7–11.Google Scholar
  20. 20.
    Su, N.Z., Toxic effects of Daphnia magna to polluted water by cadmium after treatments with aluminum chloride, Ph.D. Thesis, Shandong Normal Univ., 2013.Google Scholar
  21. 21.
    Hebert, P.H.D., The population biology of Daphnia (Crustacea, Daphnidae). Biol. Rev., 1978, vol. 53, pp. 387–426.Google Scholar
  22. 22.
    Benzie, J.A.H., The Genus Daphnia (including Daphniopsis) (Anomopoda: Daphniidae). Leiden: Backhuys Publ., 2005.Google Scholar
  23. 23.
    Wang, S.M. and Dou, H.S., China Lake, Science Press, 1998, pp. 225–229.Google Scholar
  24. 24.
    Jiang, X.Z. and Du, N.S., Fauna Sinica: Crustaceans, Freshwater Cladocera, Beijing: Science Press, 1979.Google Scholar
  25. 25.
    Xu, M., Zhang, H.J., Deng, D.G., Wang, W.P., Zhang, X.L., and Zha, L.S., Phylogenetic relationship and taxonomic status of four Daphnia species based on 16S rDNA and COI sequence, Acta Hydrobiol. Sinica, 2014, vol. 38, no. 6, pp. 1040–1046.Google Scholar
  26. 26.
    Taylor, D.J., Hebert, P.D.N., and Colbouren, J.K., Phylogenetics and evolution of the Daphnia longispina group (Crustacea) based on 12S gene sequence and allozyme variation, Mol. Phylogenet. Evol., 1996, vol. 5, no. 3, pp. 495–510.Google Scholar
  27. 27.
    Tajima, F., Statistical method for testing the neutral mutation hypothesis by DNA polymorphism, Genetics, 1989, vol. 123, no. 3, pp. 585–595.Google Scholar
  28. 28.
    Fu, Y.X., Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection, Genetics, 1997, vol. 147, no. 2, pp. 915–925.Google Scholar
  29. 29.
    Excoffier, L. and Lischer, H.E.L., Arlequin suite ver. 3.5: A new series of programs to perform population genetics analyses under Linux and Windows, Mol. Ecol. Resour., 2010, vol. 10, no. 3, pp. 564–567.Google Scholar
  30. 30.
    Yeh, F.C., Yang, R.C., and Boyle, T., POPGENE Version 1.31. Microsoft Windows-based Freeware for Population Genetic Analysis, University of Alberta and the Centre for International Forestry Research, 1999.
  31. 31.
    Pritchard, J.K., Stephens, M., and Donnelly, P., Inference of population structure using multilocus genotype data, Genetics, 2000, vol. 155, pp. 945–959.Google Scholar
  32. 32.
    Hubisz, M.J., Falush, D., Stephens, M., and Pritchard, J.K., Inferring weak population structure with the assistance of sample group information, Mol. Ecol. Resour., 2009, vol. 9, pp. 1322–1332.Google Scholar
  33. 33.
    Frisch, D., Morton, P.K., Culver, B.W., Edlund, M.B., Jeyasingh, P.D., and Weider, L.J., Paleogenetic records of Daphnia pulicaria in two North American lakes reveal the impact of cultural eutrophication, Global Change Biol., 2017, vol. 23, no. 2, pp. 708–718.Google Scholar
  34. 34.
    Ji, X.Y., Research on pollution loads and capacity of water environment of Lake Junshan, Ph.D. Thesis, Nanchang Univ., 2011.Google Scholar
  35. 35.
    Valdecasas, A.G., Camacho, A.I., and Pelaez, M.L., Do small animals have a biogeography?, Exp. Appl. Acarol., 2006, vol. 40, pp. 133–144.Google Scholar
  36. 36.
    Vujanovic, V., Hamel, C., Yergeau, E., Starnaud, M. Biodiversity and biogeography of Fusarium species from northeastern North American asparagus fields based on microbiological and molecular approaches, Microb. Ecol., 2006, vol. 51, no. 2, pp. 242–255.Google Scholar
  37. 37.
    Wang, C.T., The analysis of phylogeography and population genetic structure of the mudskipper (Boleoph-thalmuspectinirostris) in China coastal Sea, M.D. Thesis, Shanghai Ocean Univ., 2013.Google Scholar
  38. 38.
    Palumbi, S.R., Population genetics, demographic connectivity, and the design of marine reserves, Ecol. Appl., 2003, vol. 13, no. 1, pp. 146–158.Google Scholar
  39. 39.
    Ma, K.Y., Craig, M.T., Choat, J.H., and Herwerden, L.V., The historical biogeography of groupers: Clade diversification patterns and processes, Mol. Phylogenet. Evol., 2016, vol. 100, pp. 21–30.Google Scholar
  40. 40.
    Capo, E., Debroas, D., Arnaud, F., Guillemot, T., Bichet, V., Millet, L., Gauthier, E., Massa, C., Develle, A.L., Pignol, C., Lejzerowicz, F., and Domaizon, I., Long-term dynamics in microbial eukaryotes communities: A paleolimnological view based on sedimentary DNA, Mol. Ecol., 2016, vol. 25, no. 23, pp. 5925–5943.Google Scholar
  41. 41.
    Frisch, D., Morton, P.K., Chowdhury, P.R., Culver, B.W., Colbourne, J.K., Weider, L.J., and Jeyasingh, P.D., A millennial-scale chronicle of evolutionary responses to cultural eutrophication in Daphnia, Ecol. Lett., 2014, vol. 17, no. 3, pp. 360–368.Google Scholar
  42. 42.
    Xiang, X.L., Xi, Y.L., Zhu, L.Y., and Xu, Q.L., Comparative studies of the population genetic structure of the Brachionus calyciflorus species complex from four inland lakes in Wuhu, China, Biochem. Syst. Ecol., 2017, vol. 71, pp. 69–77.Google Scholar
  43. 43.
    Orsini, L., Marshall, H., Cuenca, C.M., Chaturvedi, A., and Thomas, K.W., Temporal genetic stability in natural populations of the waterflea Daphnia magna in response to strong selection pressure, Mol. Ecol., 2016, vol. 25, no. 24, pp. 6024–6038.Google Scholar

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© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  1. 1.School of Resource and Environmental EngineeringAnhui UniversityHefeiChina
  2. 2.Anhui Key Laboratory of Resource and Plant Biology, School of Life ScienceHuaibei Normal UniversityHuaibeiChina

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