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Mixotrophic Cultivation of Dunaliella salina on Crude Glycerol Obtained from Calcinated Fatty Acid Production Process


Dunaliella salina is a unicellular microalga with the potential of industrial applications. Commercial-scale cultivation of D. salina requires extensive efforts on cost reduction. This can be achieved by minimization of nutrient cost and maximization of specific growth rate and productivity. To this end, in this study, mixotrophic cultivation of D. salina on crude glycerol (by-product of calcinated-fatty acid (CaFA) production process) was investigated in shake flasks and the influences on protein, carotenoid and chlorophyll contents of the cells were studied. The highest maximum specific growth rate and biomass productivity was achieved by cultivation on 2.5 g/L crude glycerol. The protein content was more than two folds and the carotenoid content was more than 60% higher than that of autotrophic cultivation. Subsequently, continuous cultivation of D. salina on crude glycerol in a helical photobioreactor was performed at the dilution rate of 0.52/d. The biomass productivity of 2.3 × 106 cell/(mL d) was achieved which was more than 75% of the values for previously reported autotrophic cultivations. Conclusively, continuous cultivation of D. salina on by-product of CaFA production process could be a potential process technology for production of D. Salina.

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The financial support provided for this project by the Isfahan University of Technology is gratefully acknowledged.

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Correspondence to M. H. Jazini.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Sohrabi, D., Jazini, M.H. & Shariati, M. Mixotrophic Cultivation of Dunaliella salina on Crude Glycerol Obtained from Calcinated Fatty Acid Production Process. Russ J Mar Biol 45, 470–480 (2019).

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  • Dunaliella salina
  • crude glycerol
  • mixotrophic cultivation
  • fatty acid salts production process
  • calcinated fatty acid