The Influence of Secretory Products of Morula Cells on Phagocytes of the Holothurian Eupentacta fraudatrix (Djakonov et Baranova, 1958) (Sclerodactylidae: Dendrochirotida)
- 19 Downloads
The influence of humoral products of morula cells on apoptosis, the concentration of cytokinin-like compounds, and expression of cell-surface receptors specific to plant lectins from Arachis hypogaea, Glycine max, and concanavalin A (Con A) were studied in two types of phagocytes (P1 and P2) of the Far Eastern sea cucumber Eupentacta fraudatrix (Djakonov et Baranova, 1958). Our data show that morula cell supernatant reduced the level of apoptosis and concentration of interleukin-1α-like factors (IL-1α-LF) in P1 phagocytes, while it increased them in P2 phagocytes. We hypothesize that an increase in IL-1α-LF concentration stimulates apoptosis in E. fraudatrix P2 cells under the effect of treatment with morula cell supernatant. Moreover, opposite changes in apoptosis levels in P1 and P2 phagocytes in response to morula cell supernatant correlate with the expression of receptors of different types: N-acetyl-D-galactosamine- and β-D-galactose-containing receptors in P1 phagocytes and α-D-mannose-containing receptors in P2 phagocytes. Taken together, our results support the idea of differential roles of P1 and P2 phagocytes in the holothurian immune response. The study suggests that the differences in the binding of lectins to P1 and P2 cell-surface receptors is a distinctive feature and can be used in phenotyping of these immunocytes.
Keywords:cell interaction phagocytes morula cells holothurian immunity apoptosis lectins
COMPLIANCE WITH ETHICAL STANDARDS
Conflict of interest. The authors declare that they have no conflict of interest.
Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
- 1.Besednova, N.N., Marine hydrobionts – potential sources of drugs, Zdorov’e, Med. Ekol., Nauka, 2014, no. 3(57), pp. 4–10.Google Scholar
- 3.Dolmatova, L.S., Zaika, O.A., Nedashkovskaya, E.P., and Timchenko, N.F., Studying mechanisms of apoptosis-modulating activity of thermoresistant toxin Yersinia pseudotuberculosis and corrective effect of Far-Eastern holothurians-derived extract in rat neutrophils in vitro, Tikhookean. Med. Zh., 2010, no. 3(41), pp. 76–80.Google Scholar
- 4.Dolmatova, L.S., Shitkova, O.A., Dolmatov, I.Yu., and Timchenko, N.F., Thermostable lethal toxin of Yersinia pseudotuberculosis induces apoptosis and inhibits expression receptors of holothuria immunocytes to lectins, Zh. Mikrobiol., Epidemiol. Immunobiol., 2006, suppl. 3, pp. 23–28.Google Scholar
- 5.Dolmatova, L.S. and Ulanova, O.A., Variations in antioxidant enzyme activities of phagocytes and morular cells of the holothurian Eupentacta fraudatrix during cell interaction and their modulation with dexamethasone, Fundam. Issled.: Biol. Nauki, 2014, no. 5(2), pp. 276–282.Google Scholar
- 6.Dolmatova, L.S., Ulanova, O.A., Bynina, M.P., and Timchenko, N.F., Thermostable toxin Yersinia pseudotuberculosis induces opposite variations in the levels of markers of functional activities of the two types of phagocytes in the holothurian Eupentacta fraudatrix, Zdorov’e, Med. Ekol., Nauka, 2017, no. 3(70), pp. 108–111.Google Scholar
- 7.Zaporozhets, T.S., Ivanushko, L.A., Zvjagintseva, T.N., et al., Induced cytokine activity with biopolimers of marine hydrobionts, Med. Immunol., 2004, vol. 6, nos. 1–2, pp. 89–96.Google Scholar
- 9.Odintsova, N.A., Osnovy kul’tivirovaniya kletok morskikh bespozvonochnykh (Bases of Cultivation of Marine Invertebrate Cells), Vladivostok: Dal’nauka, 2001.Google Scholar
- 10.Pavlovskaya, N.E. and Gagarina, I.N., The physiological properties of plant lectins as a prerequisite for their application in biotechnology, Khim. Rastit. Syr’ya, 2017, no. 1, pp. 21–35.Google Scholar
- 13.Sukhachev, A.N., Kudryavtsev, I.V., Nikolaev, K.E., et al., Influence of lectins of various carbohydrate specificity on hemolytic activity of hemocytes in the mussel Mytilus edulis, in Mater. XXVIII Mezhdunar. Konf. “Biologicheskie resursy Belogo morya i vnutrennikh vodoemov Evropeiskogo Severa”, 5–8 oktyabrya 2009 g. (Proc. XXVIII Int. Conf. “Biological Resources of the White Sea and Inland Waters of the European North”, October 5–8, 2009), Petrozavodsk: Karel’skii Nauchn. Tsentr, Ross. Akad. Nauk, 2009, pp. 538–542.Google Scholar
- 14.Chernikov, O.V., Chikalovets, I.V., Molchanova, V.I., and Lukyanov, P.A., Biological activity of lectins of marine hydrobionts, Vestn. Dal’nevost. Otd., Ross. Akad. Nauk, 2007, no. 6, pp. 131–135.Google Scholar
- 15.Chechina, O.E., Biktasova, A.K., Sazonova, E.V., et al., Role of cytokines in the redox-dependent regulation of apoptosis, Byull. Sib. Med., 2009, vol. 8, no. 2, pp. 67–72.Google Scholar
- 17.Belogortseva, N.I., Molchanova, V.I., Kurika, A.V., et al., Isolation and characterization of new GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus, Comp. Biochem. Physiol., Part C: Pharmacol., Toxicol. Endocrinol., 1998, vol. 119, no. 1, pp. 45–50.Google Scholar
- 19.Chia, F. and Xing, J., Echinoderm coelomocytes, Zool. Stud., 1996, vol. 35, no. 4, pp. 231–254.Google Scholar
- 25.Seco-Rovira, V., Beltrán-Frutos, E., Ferrer, C., et al., Lectin histochemistry as a tool to identify apoptotic cells in the seminiferous epithelium of Syrian hamster (Mesocricetus auratus) subjected to short photoperiod, Reprod. Domest. Anim., 2013, vol. 48, no. 6, pp. 974–983.CrossRefPubMedGoogle Scholar