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Journal of Analytical Chemistry

, Volume 74, Issue 11, pp 1057–1063 | Cite as

Preconcentration of Catecholamins on Hypercrosslinked Polystyrene and Their Determination by High-Performance Liquid Chromatography

  • V. V. Tolmacheva
  • D. I. Yarykin
  • M. V. Gorbunova
  • V. V. Apyari
  • S. G. DmitrienkoEmail author
  • Yu. A. Zolotov
ARTICLES
  • 16 Downloads

Abstract

The use of hypercrosslinked polystyrene for the group sorption preconcentration of catecholamines is proposed. The conditions for the preconcentration of noradrenaline, adrenaline, and dopamine are optimized: a microcolumn (10 × 6 mm), mass of adsorbent 0.03 g, 25 mL of solution (pH ~ 8.5), and transmission rate of the solution 1.0 mL/min. Compounds were desorbed with 1 mL of 6 M acetic acid and determined in the eluate by reversed-phase HPLC with an amperometric detector (E = 0.8 V). Adsorption preconcentration ensures the reduction of the limits of detection for catecholamines by more than 20 times. The limits of detection were 0.7 ng/mL for dopamine and 1 ng/mL for noradrenaline and adrenaline. The procedure was applied to the analysis of model mixtures based on urine.

Keywords:

adrenaline noradrenaline dopamine solid phase extraction hypercrosslinked polystyrene HPLC 

Notes

FUNDING

The work was performed with the financial support of the Russian Science Foundation, project no. 18-73-10001 using equipment acquired from the funds of the Moscow University Development Program.

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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • V. V. Tolmacheva
    • 1
    • 2
  • D. I. Yarykin
    • 1
  • M. V. Gorbunova
    • 1
  • V. V. Apyari
    • 1
  • S. G. Dmitrienko
    • 1
    Email author
  • Yu. A. Zolotov
    • 1
    • 2
  1. 1.Department of Chemistry, Moscow State UniversityMoscowRussia
  2. 2.Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of SciencesMoscowRussia

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