Advertisement

Journal of Analytical Chemistry

, Volume 74, Issue 7, pp 707–714 | Cite as

An Electrochemical DNA Sensor for Doxorubicin Based on a Polyelectrolyte Complex and Aminated Thiacalix[4]Arene

  • A. V. Porfir’evaEmail author
  • K. S. Shibaeva
  • V. G. Evtyugin
  • L. S. Yakimova
  • I. I. Stoikov
  • G. A. Evtyugin
ARTICLES
  • 1 Downloads

Abstract

An impedimetric DNA sensor is developed for the highly sensitive determination of doxorubicin; the signal is charge transfer resistance recorded by electrochemical impedance spectroscopy using a glassy carbon electrode modified by electropolymerized Neutral Red or polyaniline and polyelectrolyte complexes including DNA. The nature of the polymer layer and the composition of the polyelectrolyte complex affect the sensitivity of the determination of doxorubicin. The role of a macrocycle capable of shielding the DNA negative charge and interacting with cationic centers of the substrate is shown. With an optimal composition of polyelectrolytes, the DNA sensor ensures the determination of down to 0.1 nM of doxorubicin.

Keywords:

DNA sensor biosensor electropolymerization Neutral Red polyaniline DNA intercalation determination of doxorubicin 

Notes

FUNDING

This work was supported by the Russian Science Foundation, project no. 17-73-20024.

REFERENCES

  1. 1.
    Lubert, K.H. and Kalcher, K., Electroanalysis, 2010, vol. 22, nos. 17–18, p. 1937.CrossRefGoogle Scholar
  2. 2.
    Rao, G. and Goyal, A., Pharm. Chem. J., 2016, vol. 3, no. 2, p. 280.Google Scholar
  3. 3.
    Maragos, C.M. and Busman, M., Food Addit. Contam., Part A, 2010, vol. 27, no. 5, p. 688.Google Scholar
  4. 4.
    Da Silva, E.T.S.G., Souto, E.P., Barragan, J.T.C., Giarola, J.F., de Moralis, A.C.M., and Kubota, L.T., ChemElectroChem., 2017, vol. 4, no. 4, p. 778.CrossRefGoogle Scholar
  5. 5.
    Kimmel, D.W., Le Blanc, G., Meschievitz, M.E., and Cliffel, D., Anal. Chem., 2012, vol. 84, no. 2, p. 685.CrossRefGoogle Scholar
  6. 6.
    Rashid, J.I.A. and Yusof, N.A., Sens. Bio-Sens. Res., 2017, vol. 16, no. 1, p. 19.CrossRefGoogle Scholar
  7. 7.
    Yu, H.L.L., Maslova, A., and Hsing, I-M., ChemElectroChem., 2017, vol. 4, no. 4, p. 795.CrossRefGoogle Scholar
  8. 8.
    Yang, F., Teves, S.S., Kemp, C.J., and Henikoff, S., Biochim. Biophys. Acta, 2014, vol. 1845, no. 1, p. 84.Google Scholar
  9. 9.
    Evtugyn, G.A., Stepanova, V.B., Porfireva, A.V., Zamaleeva, A.I., and Fakhrullin, R.R., J. Nanosci. Nanotechnol., 2014, vol. 14, no. 9, p. 6738.CrossRefGoogle Scholar
  10. 10.
    Hao, X., Xu, Z., Li, N., Li, N.B., and Luo, H.Q., Anal. Methods, 2015, vol. 7, no. 9, p. 3849.CrossRefGoogle Scholar
  11. 11.
    Sun, Y., Wang, J., Jin, L., Chang, Y., Duan, J., and Lu, Y., Polym. J., 2015, vol. 47, p. 753.CrossRefGoogle Scholar
  12. 12.
    Jeong, J.-E. and Woo, H.Y., Polym. Chem., 2017, vol. 8, p. 6329.CrossRefGoogle Scholar
  13. 13.
    Ensafi, A.A., Amini, M., and Rezaei, B., Sens. Actuators, B, 2013, vol. 177, p. 862.CrossRefGoogle Scholar
  14. 14.
    Evtugyn, G., Porfireva, A., Stepanova, V., and Budnikov, H., Electroanalysis, 2015, vol. 27, no. 3, p. 629.CrossRefGoogle Scholar
  15. 15.
    Evtugyn, G. and Hianik, T., TrAC, Trends Anal. Chem., 2016, vol. 79, no. 1, p. 168.CrossRefGoogle Scholar
  16. 16.
    Shamagsumova, R., Porfireva, A., Stepanova, V., Osin, Yu., Evtugyn, G., and Hianik, T., Sens. Actuators, B, 2015, vol. 220, p. 573.CrossRefGoogle Scholar
  17. 17.
    Pauliukaite, R., Ghica, M.E., Barsan, M., and Brett, C.M.A., J. Solid State Electrochem., 2007, vol. 11, no. 7, p. 899.CrossRefGoogle Scholar
  18. 18.
    Schlereth, D.D. and Karyakin, A.A., J. Electroanal. Chem., 1995, vol. 395, nos. 1–2, p. 221.CrossRefGoogle Scholar
  19. 19.
    Zhang, K. and Zhang, Y., Electroanalysis, 2010, vol. 22, no. 6, p. 673.CrossRefGoogle Scholar
  20. 20.
    Yakimova, L.S., Puplampu, J.B., Evtyugin, G.A., and Stoikov, I.I., Russ. Chem. Bull., 2017, vol. 66, no. 8, p. 1515.CrossRefGoogle Scholar
  21. 21.
    Stoikov, I.I., Galukhin, A.V., Zaikov, E.N., and Antipin, I.S., Mendeleev Commun., 2009, vol. 19, no. 4, p. 193.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • A. V. Porfir’eva
    • 1
    Email author
  • K. S. Shibaeva
    • 1
  • V. G. Evtyugin
    • 2
  • L. S. Yakimova
    • 1
  • I. I. Stoikov
    • 1
  • G. A. Evtyugin
    • 1
  1. 1.Butlerov Institute of Chemistry, Kazan Federal UniversityKazanRussia
  2. 2.Interdisciplinary Center for Analytical Microscopy, Kazan Federal UniversityKazanRussia

Personalised recommendations