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Journal of Analytical Chemistry

, Volume 74, Issue 7, pp 659–671 | Cite as

“Fit-for-Purpose” Simultaneous Extraction of Water-Soluble Vitamins (PP, B2, B6, B9, B5) from Mineral Feeds and Premixes and Simultaneous Determination of B1 and Nicotinic Acid

  • Fabrizia ForghieriEmail author
  • Biagio Formisano
  • Lucia Casarin
  • David Latini
  • Rita Maria Maestro
ARTICLES
  • 6 Downloads

Abstract

A fast quantitative fit-for-purpose method has been developed for the simultaneous extraction of the water-soluble vitamins: nicotinamide (PP), riboflavin (B2), pyridoxine (B6), folic acid (B9) and pantothenic acid (B5) from mineral feeds and premixes, simultaneously determined together with thiamine (B1) and nicotinic acid. The single extractant for vitamins B2, B6, nicotinamide, B9 and B5 is a neutral buffer. The vitamins are separated and determined by ion-pair HPLC with a long (150 mm) C18 column using a mobile phase consisting of a gradient of phosphate buffer at pH 3 (phase A) and methanol (phase B) with selective detection at 214, 260 and 294 nm. A statistical study was conducted to assess recovery and uncertainty to verify fit-for-purpose characteristic of the method. Linearity, accuracy, repeatability, robustness, LOQ and LOD were also determined.

Keywords:

selective extraction water-soluble vitamins fit-for-purpose extraction tolerances of Regulation (EU) no. 939/2010 mineral feeds premixes ion-pair HPLC−UV 

REFERENCES

  1. 1.
    Caselli, R., Mangimi composti integrati: manuale teorico-pratico per il mangimista e per l’allevatore (Integrated Compound Feed: Theoretical Practical Manual for Animal Feeding Advisers and Breeders), Bologna: Edagricole, 1981.Google Scholar
  2. 2.
    Arbatskii, A.P., Afon’shin, G.N., and Vostokov, V.N., J. Anal. Chem., 2004, vol. 59, no. 12, p. 1304.CrossRefGoogle Scholar
  3. 3.
    Rudenko, A.O. and Kartsova, L.A., J. Anal. Chem., 2010, vol. 65, no. 1, p. 71.CrossRefGoogle Scholar
  4. 4.
    Ndaw, S., Bergaentzlè, M., Aoudè-Werner, D., and Hasselmann, C., Food Chem., 2000, vol. 71, p. 129.CrossRefGoogle Scholar
  5. 5.
    Esteve, M.J., Farré, R., Frígola, A., and García-Cantabella, J.M., J. Chromatogr. A, 1998, vol. 795, p. 383.CrossRefGoogle Scholar
  6. 6.
    Heudi, O., Kilinç, T., and Fontannaz, P., J. Chromatogr. A, 2005, vol. 1070, p. 49.CrossRefGoogle Scholar
  7. 7.
    Moreno, P. and Salvadó, V., J. Chromatogr. A, 2000, vol. 870, p. 207.CrossRefGoogle Scholar
  8. 8.
    Engel, R., Stefanovits-Bányai, E., and Abrankó, L., Chromatographia, 2010, vol. 71, p. 1069.CrossRefGoogle Scholar
  9. 9.
    AOAC 940.33: Riboflavin (Vitamin B2) in Vitamin Preparation,1960.Google Scholar
  10. 10.
    AOAC 944.13: Niacin and Niacinamide (Nicotinic Acid and Nicotinamide), 1960.Google Scholar
  11. 11.
    AOAC 961.15: Vitamin B6 (Pyridoxine, Pyridoxal, Pyridoxamine), 1975.Google Scholar
  12. 12.
    AOAC 944.12, 960.46: Folic Acid, 1960.Google Scholar
  13. 13.
    AOAC 945.74: Pantothenic Acid in Vitamin Preparation, 1990.Google Scholar
  14. 14.
    Svidritskii, E.P., Pashkova, E.B., Pirogov, A.V., and Shpigun, O.A., J. Anal. Chem., 2010, vol. 65, no. 3, p. 287.CrossRefGoogle Scholar
  15. 15.
    EC Commission Regulation no. 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed, Off. J. Eur. Communities: Legis., 2009, vol. L54, p. 1.Google Scholar
  16. 16.
    Approval of the official test method for the determination of vitamin D3 in animal feeds, Decree of 17 July 2012, Italian Official Gazette, August 3, 2012, no. 180, General Series.Google Scholar
  17. 17.
    Approval of the official test method for the determination of vitamin D3 in animal feeds, Decree of 29 April 2010, Italian Official Gazette, May 25, 2010, no. 120, General Series.Google Scholar
  18. 18.
    Approval of the official test method for the determination of vitamin D3 in animal feeds, Decree of February 20, 2006, Italian Official Gazette, March 3, 2006, no. 50, suppl. no. 19.Google Scholar
  19. 19.
    Modern Chromatographic Analysis of Vitamins, De Leenheer, A.P., Lambert, W.E., and Nelis, H.J., Eds., New York: Marcel Decker, 1992, 2nd ed.Google Scholar
  20. 20.
    Rienzi, R., Metodi di analisi delle vitamine (Methods of Vitamins Assay), Pinerolo, Italy: Chiriotti, 1976.Google Scholar
  21. 21.
    Frost, D.V., US Patent 2388261, 1945.Google Scholar
  22. 22.
    UNI ISO 5725-1:2004 Accuracy (Trueness and Precision) of Measurement Method and Results, parts 1–6, part 1.Google Scholar
  23. 23.
    UNI CEI ENV 13005 (ex UNI CEI 9/1997), Guide to the Expression of Uncertainty in Measurement (GUM). Google Scholar
  24. 24.
    ACCREDIA (Italian National Accreditation Body): Guide to the Evaluation and Expression of Uncertainty in Measurement, document DT 0002 rev. 1.Google Scholar
  25. 25.
    ACCREDIA (Italian National Accreditation Body): Guidelines for the Evaluation of Uncertainty in the Field of Chemical Analysis, document DT 0002/3 rev. 0.Google Scholar
  26. 26.
    ACCREDIA (Italian National Accreditation Body): Guide to the Calculation of the Repeatability of a Method and Verification Thereof over Time, document DT 0002/6 rev. 0.Google Scholar
  27. 27.
    Decree of 21 February 2001, Italian Official Gazette, June 5, 2001, no. 128, General Series.Google Scholar
  28. 28.
    Handbook of Vitamins, Machlin, L.J., Ed., New York: Marcel Dekker, 1991, 2nd ed.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • Fabrizia Forghieri
    • 1
    Email author
  • Biagio Formisano
    • 1
  • Lucia Casarin
    • 1
  • David Latini
    • 1
  • Rita Maria Maestro
    • 1
  1. 1.ICQRF-Ispettorato Centrale della Tutela della Qualità e Repressione Frodi dei Prodotti Agro-Alimentari (Central Inspectorate for the Protection of Quality and the Suppression of Fraud in Agri-food Products)–MIPAAFT (Ministry of Agricultural, Food, Tourism and Forestry Policies)RomeItaly

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