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Glutamate sol-gel amperometric biosensor based on co-immobilised NADP+ and glutamate dehydrogenase

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Abstract

In this work the preparation and electrochemical characterisation of a new glutamate amperometric sol-gel biosensor is described. A carbon paste electrode was electrochemically modified with the phenothiazine dye methylene green (MG). The NADP+ and glutamate dehydrogenase were co-immobilised in a sol-gel matrix. When coupled to a flow injection system (FIA) the biosensor showed good electrocatalytic activity towards NADPH oxidation at the potential of + 0.3 V vs. Ag/AgCl; KCl sat., which represents a strong overpotential reduction. The biosensor yielded a linear response from 5.0 × 10−5 to 1.0 × 10−2 M glutamate concentration, with a detection limit of 5 × 10−6 M and reproducibility of results better than 2.3% (RSD). Moreover, the implemented biosensor showed to be still useful after 7 months, 500 determinations.

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Authors and Affiliations

  1. Requimte, Departamento de Química, Fac. Farmácia, Universidade do Porto Rua Anibal, Cunha, 164, Porto, 4099-030, Portugal

    S. P. Gomes, A. N. Araújo, C. M. C. M. Couto & M. C. B. S. M. Montenegro

  2. Dep. Analytical Chemistry, Fac. Pharmacy, Charles University, Charles, Czech Republic

    J. Doležalová

  3. Centro Investigação Ciências da Saúde (CICS)/ISCS-Norte-Rua Central da Gandra-1317 GRD, Paredes, Portugal

    C. M. C. M. Couto

Authors
  1. S. P. Gomes
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  2. J. Doležalová
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  3. A. N. Araújo
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  4. C. M. C. M. Couto
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  5. M. C. B. S. M. Montenegro
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Gomes, S.P., Doležalová, J., Araújo, A.N. et al. Glutamate sol-gel amperometric biosensor based on co-immobilised NADP+ and glutamate dehydrogenase. J Anal Chem 68, 794–800 (2013). https://doi.org/10.1134/S1061934813090049

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  • DOI: https://doi.org/10.1134/S1061934813090049

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