Russian Journal of Electrochemistry

, Volume 55, Issue 10, pp 933–942 | Cite as

Electrochemical Determination of Levodopa on Carbon Paste Electrode Modified with Salmon Sperm DNA and Reduced Graphene Oxide–Fe3O4 Nanocomposite

  • M. Hosseini GhalehnoEmail author
  • M. Mirzaei
  • M. Torkzadeh-Mahani


In this work, an electrochemical label-free DNA biosensor was developed for determination of levodopa (LD). The biosensor was constructed using reduced graphene oxide decorated with Fe3O4 magnetic nanoparticles (rGO–Fe3O4) on a carbon paste electrode (CPE) and double-stranded deoxyribonucleic acid (DNA) (DNA/rGO–Fe3O4-CPE). The application was related to the molecular interaction between LD and DNA. Thus, the voltammetric behavior of LD at the surface of DNA/rGO–Fe3O4-CPE was studied using differential pulse voltammetry (DPV) where the oxidation peak current of LD was measured as an analytical signal. A considerable increase was observed in the oxidation signal of LD at the DNA-coated electrode compared to the DNA-free electrode, indicating the pre-concentration of LD due to the interaction with the surface-confined DNA layer. Scanning electron microscopy, energy dispersive X-ray and Fourier transform infrared spectroscopy confirmed the structure of the synthesized nanocomposites (electrode composition). Electrochemical studies revealed that modification of the electrode significantly increases the oxidation peak currents of LD. Under the optimum conditions, the calibration curve was linear in the range of 0.5–600 nM with a detection limit of 0.11 nM. The relative standard deviation for 200.0 nM was 4.07% (n = 5). The developed biosensor was successfully applied to the analysis of LD in human serum and urine sample.


biosensor carbon paste electrode levodopa nanocomposite 



The authors wish to thank the Shahid Bahonar University of Kerman and Graduate University of Advanced Technology of Kerman.


The authors declare that they have no conflict of Interest.

Supplementary material

11175_2019_1048_MOESM1_ESM.pdf (328 kb)


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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • M. Hosseini Ghalehno
    • 1
    • 2
    Email author
  • M. Mirzaei
    • 1
  • M. Torkzadeh-Mahani
    • 3
  1. 1.Department of Chemistry, Shahid Bahonar University of KermanKermanIran
  2. 2.Young Research Society, Shahid Bahonar University of KermanKermanIran
  3. 3.Department of Biotechnology, Institute of Science, High Technology and Environmental Sciences, Graduate University of Advanced TechnologyKermanIran

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