Russian Journal of Genetics

, Volume 55, Issue 11, pp 1417–1425 | Cite as

Variability of the North Caucasian Populations of the Greenbug for Host Virulence and Discovered by Molecular Markers

  • E. E. RadchenkoEmail author
  • N. V. Alpatieva
  • M. A. Chumakov
  • R. A. Abdullaev


Patterns of the genetic structure dynamics of Krasnodar and Dagestan populations of the greenbug, Schizaphis graminum Rondani, in terms of host virulence and molecular markers were examined. High seasonal polymorphism of two North Caucasian insect populations in terms of virulence frequencies to sorghum accessions protected by the major resistance genes was revealed. It was demonstrated that this variability also depended on the plant resistance genes with weak phenotypic manifestation. During feeding on sorghum, the frequencies of greenbug clones virulent to barley accessions also changed. Moreover, the selection of clones on susceptible and moderately resistant sorghum accessions was differential. On the basis of pyrosequencing of a polymorphic fragment of the mitochondrial ND4 gene, 16 greenbug haplotypes were identified. The heterogeneity and difference of the North Caucasian insect populations by the ratio of the identified haplotypes was demonstrated. In the Krasnodar population of S. graminum, both aphid clone samples sampled simultaneously on the phytophage-susceptible and moderately resistant sorghum genotypes and those sampled at different vegetation periods on the same host plant were different.


sorghum barley Sсhizaphis graminum populations virulence mitochondrial DNA ND4 haplotypes selection 



This study was supported by the budget financing within the scope of the state contract with VIR (no. 0662-2019-0006).


The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.


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Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  • E. E. Radchenko
    • 1
    Email author
  • N. V. Alpatieva
    • 1
  • M. A. Chumakov
    • 1
  • R. A. Abdullaev
    • 1
  1. 1.Federal Research Center Vavilov All-Russian Institute of Plant Genetic Resources (VIR)St. PetersburgRussia

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