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Russian Journal of Genetics

, Volume 55, Issue 11, pp 1315–1329 | Cite as

Genetic Heterogeneity of a Diploid Grass Aegilops tauschii Revealed by Chromosome Banding Methods and Electrophoretic Analysis of the Seed Storage Proteins (Gliadins)

  • E. D. BadaevaEmail author
  • A. V. Fisenko
  • S. A. Surzhikov
  • A. A. Yankovskaya
  • N. N. Chikida
  • S. A. Zoshchuk
  • M. Kh. Belousova
  • A. Yu. Dragovich
PLANT GENETICS

Abstract

Genetic diversity of diploid grass Ae. tauschii Coss (2n = 2x = 14, DD), the D-genome progenitor of common wheat, was assessed using fluorescence in situ hybridization (FISH) with eleven DNA probes representing satellite and microsatellite DNA sequences as well as the 45S and 5S rRNA gene families and by electrophoretic (EF) analysis of seed storage proteins (gliadins). A clear genetic differentiation of accessions into groups strangulata (Str) and tauschii (Tau) was observed. The groups differed in the presence of microsatellite repeats GAAn and ACTn and in the distribution of satellite DNA families, especially pAs1. On the basis of similarities of labeling patterns of DNA probes used in the study, we concluded that the Str group was phylogenetically closest to the D genome of common wheat. A comparison of spectra of gliadins revealed the highest similarity of Armenian and Azerbaijani accessions of Ae. tauschii to common wheat, which may indicate a contribution of Transcaucasian members of the Str group in the formation of the genetic pool of common wheat.

Keywords:

Aegilops tauschii D genome chromosome FISH repeated DNA sequences seed storage proteins (gliadins) evolution 

Notes

ACKNOWLEDGMENTS

The accessions were obtained from the collection of genetic plant resources at the Federal Research Center Vavilov All-Russian Institute of Plant Genetic Resources (St. Petersburg, Russia), the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) (Gatersleben, Germany), USDA-ARS (Aberdeen, Idaho, USA), and the Weizmann Institute of Science (Rehovot, Israel).

FUNDING

The present study was supported by the budget projects of the Vavilov Institute of General Genetics of the Russian Academy of Sciences (no. 0112-2019-0002) and the Federal Research Center Vavilov All-Russian Institute of Plant Genetic Resources (no. 0662-2019-0006) and received partial support from the Russian Foundation for Basic Research (project no. 17-04-00087).

COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  • E. D. Badaeva
    • 1
    • 2
    Email author
  • A. V. Fisenko
    • 3
  • S. A. Surzhikov
    • 2
  • A. A. Yankovskaya
    • 1
  • N. N. Chikida
    • 4
  • S. A. Zoshchuk
    • 2
  • M. Kh. Belousova
    • 4
  • A. Yu. Dragovich
    • 1
  1. 1.Vavilov Institute of General Genetics, Russian Academy of SciencesMoscowRussia
  2. 2.Engelhardt Institute of Molecular Biology, Russian Academy of SciencesMoscowRussia
  3. 3.Tsitsin Main Moscow Botanical Garden, Russian Academy of SciencesMoscowRussia
  4. 4.Federal Research Center Vavilov All-Russian Institute of Plant Genetic ResourcesSt. PetersburgRussia

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