Advertisement

Russian Journal of Genetics

, Volume 54, Issue 12, pp 1466–1470 | Cite as

Possible Causes and Consequences of the Distribution of Separate Allelic Variants of the LEP Gene in the Groups of Ayrshire and Holstein Cattle

  • N. V. KovalyukEmail author
  • V. F. SatsukEmail author
  • E. V. Machulskaya
  • Yu. Yu. Shakhnazarova
ANIMAL GENETICS
  • 14 Downloads

Abstract

PCR/RFLP technology was implemented to identify genotypes for the Y7F, A80V, R25C loci of the leptin gene in 912 cattle of Holstein and Ayrshire breeds. This analysis revealed polymorphism for SNP allele Y7F (A → T substitution at Y7F site at position 95 689 996 bp in LEP gene), which is practically absent in Holstein animals (n = 410), where the frequency of occurrence of allele F is 0.01. In contrast, in the Ayrshire animal group (n = 502), Y7F SNP polymorphism is common, with the frequency of the occurrence of allele F at 0.25, while A80V SNP allele polymorphism for this gene (C → T substitution at the position 95 691 973 bp of the leptin gene) was not detected. In Ayrshire cattle, the FF genotype (at the Y7F site) was detected only in combination with the RRAA genotype (T → C substitution at the R25C site at position 95 690 050 bp in the LEP gene) in 100% of all possible combinations of genotypes (FFCCAA, FFRCAA and FFRRAA). In addition, genotype VV (site A80V) was detected in Holstein cattle in combination with the YYCC genotype (Y7F, R25C sites) in 100% of all possible combinations of the genotypes (YYRCVV, YYRRVV, YYCCVV). We also found that Ayrshire cattle displaying RR genotype at the LEP R25C locus are more likely to have a longer productive life than animals with other genotypes (in particular, with the CC genotype at the R25C locus). Taken together, we conclude that A80V polymorphism affects the predicted transmitted ability for milk production (PTAM), the fertility index of daughters (FI), and the pregnancy rate of daughters in Holstein cattle (DPR).

Keywords:

leptin gene A80V locus R25C locus locus Y7F frequency of occurence 

Notes

REFERENCES

  1. 1.
    Komisarek, J., Impact of LEP and LEPR gene polymorphisms on functional traits in Polish Holstein-Friesian cattle, Anim. Sci. Pap. Rep., 2010, vol. 10, pp. 133—141.Google Scholar
  2. 2.
    Yoon, D.H., Cho, B.H., and Park, B.L., et al., Highly polymorphic bovine leptin gene, J. Anim. Sci., 2005, vol. 18, no. 11, pp. 1548—1551.Google Scholar
  3. 3.
    Kononoff, P.J., Defoor, P.J., and Engler, M.J., et al., Impacts of a leptin SNP on growth performance and carcass characters in finishing steers studied over time, J. Anim. Sci., 2017, vol. 95, no. 1, pp. 194—200. doi 10.2527/jas.2016.0926CrossRefGoogle Scholar
  4. 4.
    Komisarek, J., Szyda, J., Michalak, A., and Dorynek, Z., Impact of leptin gene polymorphisms on breeding value for milk production traits in cattle, J. Anim. Feed Sci., 2005, vol. 14, pp. 491—500.CrossRefGoogle Scholar
  5. 5.
    Szyda, J. and Komisarek, J., Statistical modeling of candidate gene effects on milk production traits in dairy cattle, J. Dairy Sci., 2007, vol. 90, pp. 2971—2979.CrossRefGoogle Scholar
  6. 6.
    Trakovická, A., Moravčíková, N., and Kasarda, R., Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle, Acta Biochim. Pol., 2013, vol. 60, no. 4, pp. 783—787.Google Scholar
  7. 7.
    Giblin, L., Butler, S., Kearney, B., et al., Association of bovine leptin polymorphisms with energy output and energy storage traits in progeny tested Holstein-Friesian dairy cattle sires, BMC Genet., 2010, no. 11, p. 73. doi 10.1186/1471-2156-11-73Google Scholar
  8. 8.
    Kovalyuk, N.V., Satsuk, V.F., Volchenko, A.E., and Machul’skaya, E.V., LEP gene allelic polymorphism in a subpopulation of Ayrshire cattle, Russ. J. Genet., 2015, vol. 51, no. 2, pp. 214—217.CrossRefGoogle Scholar
  9. 9.
    Szyda, J., Morek-Kopec, M., Komisarek, J., and Zarnecki, A., Evaluation markers in selected genes for association with functional longevity of dairy cattle, BMC Genet., 2011, no. 10, pp. 12—30. doi 10.1186/1471-2156-12-30Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  1. 1.Krasnodar Research Centre for Animal Husbandry and Veterinary MedicineKrasnodarRussia
  2. 2.LLC Research and Production Association Yug-PlemKrasnodarRussia

Personalised recommendations