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Russian Journal of Plant Physiology

, Volume 66, Issue 4, pp 609–617 | Cite as

Analysis of a New Strain of Pseudomuriella engadinensis (Sphaeropleales, Chlorophyta) for Possible Use in Biotechnology

  • Y. I. MaltsevEmail author
  • I. A. Maltseva
  • M. S. Kulikovskiy
  • S. Yu. Maltseva
  • R. A. Sidorov
RESEARCH PAPERS
  • 11 Downloads

Abstract

The species diversity of green coccoid algae is often difficult to study using light microscopy due to their complex morphology. Algae, which have morphological and ecological similarities with the genera Bracteacoccus Tereg., Dictyococcus Gerneck, and Pseudomuriella Hanagata, are one of the most taxonomically complex examples. Often, only a comparison of nucleotide sequences allows one to carry out a reliable identification and find out the phylogenetic identity of the studied objects. Pseudomuriella belongs to the group of genetically diverse genera with a homogeneous Bracteacoccus-like morphology. The combined use of two genetic markers (18S rDNA nuclear gene and chloroplast rbcL gene), along with light microscopy and comparison of fatty acid profiles, allowed the identification of the green algae strain isolated from the soil of the artificial pine and robinia plantation in the steppe zone of Ukraine as Pseudomuriella engadinensis (Kol et Chodat) Fučiková, Rada et Lewis—the first find of the species in the flora of algae in Ukraine. Analysis of the fatty acid composition of the studied strain’s cells showed that the total lipid content in the stationary growth phase was at the level of 87.9 ± 2.1 mg/g dry cell mass, and the main fatty acids were palmitic, hexadecadienoic, ruganic, oleic, linoleic, and α-linolenic—their share accounted for 82.4% of the total amount of fatty acids.

Keywords:

Pseudomuriella coccoid algae cryptic species new finds flora of Ukraine 18S rDNA rbcfatty acid composition 

Notes

FUNDING

This publication is based on research carried out with financial support by Russian Science Foundation (project number 18-74-00095).

COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Supplementary material

11183_2019_8066_MOESM1_ESM.pdf (913 kb)
11183_2019_8066_MOESM1_ESM.pdf

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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • Y. I. Maltsev
    • 1
    • 2
    Email author
  • I. A. Maltseva
    • 2
  • M. S. Kulikovskiy
    • 1
  • S. Yu. Maltseva
    • 1
  • R. A. Sidorov
    • 1
  1. 1.Timiryazev Institute of Plant Physiology, Russian Academy of ScienceMoscowRussia
  2. 2.Bohdan Khmelnytskyi Melitopol State Pedagogical UniversityMelitopolUkraine

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