Russian Journal of Plant Physiology

, Volume 66, Issue 3, pp 488–494 | Cite as

Effects of Silver Nanoparticles and Silver Nitrate on Antioxidant Responses in Echium amoenum

  • F. Abbasi
  • R. JameiEmail author


Nanoparticles have entered agriculture and biology fields because of their special effects and unique features. One of the most important properties of silver compounds is its disinfecting. The Echium amoenum plants were grown with diurnal regime of 16 h light and 8 h dark at 22–29°C and 150 μmol/(m2 s) light gravity. Plants were treated for 20 days in 7 groups including I—control, II—silver nitrate (25 ppm), III—silver nanoparticles (25 ppm), IV—silver nanoparticles plus silver nitrate (25 ppm), V—silver nitrate (50 ppm), VI—silver nanoparticles (50 ppm) and VII—silver nanoparticles plus silver nitrate (50 ppm). Results showed that anthocyanins content in groups II, III, V, VI and VII were significantly increased. Total flavonoid content was significantly increased under all treatments compared with untreated plants. Increased total phenol content was observed upon plant exposure to II, IV, V, VI and VII. Regarding the lipid peroxidation, the plants in II and VII groups showed a remarkable increase of malondialdehyde. Also result showed that DPPH free radical scavenging activity was significantly increased in IV, V, VI and VII groups. These findings correlate with the nanoparticles (NPs) causing increased production of reactive oxygen species.


Echium amoenum silver nanoparticles silver nitrate antioxidant 



The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.


  1. 1.
    Oberdörster, G., Maynard, A., Donaldson, K., Castranova, V., Fitzpatrick, J., Ausman, K., Carter, J., Karn, B., Kreyling, W., Lai, D., Olin, S., Monteiro-Riviere, N., Warheit, D., and Yang, H., Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy, Part. Fibre Toxicol., 2005, vol. 2, no. 1: 8.Google Scholar
  2. 2.
    Choi, O., Deng, K.K., Kim, N.J., Ross, L., Surampalli, R.Y., and Hu, Z., The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth, Water Res., 2008, vol. 42, pp. 3066–3074.CrossRefGoogle Scholar
  3. 3.
    Luoma, S.N., Silver nanotechnologies and the environment: old problems or new challenges? Project on Emerging Nanotechnologies Rep., 2008, vol. 15, pp. 10–57.Google Scholar
  4. 4.
    Fereidoonimehr, A., Effect of benzyladenine, nano-silver and 8-hydroxyquinolin sulfate on increasing survival of Dianthus caryophyllus (cv. Crem Viana), PhD Thesis, Tehran, 2010.Google Scholar
  5. 5.
    Akter, M., Sikder, M.T., Rahman, M.M., Ullah, A.A., Hossain, K.F.B., Banik, S., Hosokawa, T., Saito, T., and Kurasaki, M., A systematic review on silver nanoparticles-induced cytotoxicity: physicochemical properties and perspectives, J. Adv. Res., 2018, vol. 9, pp. 1–16.CrossRefGoogle Scholar
  6. 6.
    Medicinal Plants: Industrial Echium amoenum, Emad, M., Gheibi, F., Rasuli, M., Khanjanzadeh, R.V., and Jozani, M., Eds., Tehran: Poneh Publ., 2012.Google Scholar
  7. 7.
    Ghassemi, N., Sajjadi, S.E., Ghannadi, A., Shams-Ardakani, M., and Mehrabani, M., Volatile constituents of amedicinal plant of Iran, Echium amoenim Fisch. and CA Mey, DARU J. Pharm. Sci., 2003, vol. 11, pp. 32–33.Google Scholar
  8. 8.
    Sharma, P., Bhatt, D., Zaidi, M., Saradhi, P.P., Khanna, P., and Arora, S., Silver nanoparticle-mediated enhancement in growth and antioxidant status of Brassica juncea, Appl. Biochem. Biotechnol., 2012, vol. 167, pp. 2225–2233.CrossRefGoogle Scholar
  9. 9.
    Rapisarda, P., Fanella, F., and Maccarone, E., Reliability of analytical methods for determining anthocyanins in blood orange juices, J. Agric. Food Chem., 2000, vol. 48, pp. 2249–2252.CrossRefGoogle Scholar
  10. 10.
    Marinova, D., Ribarova, F., and Atanassova, M., Total phenolics and total flavonoids in Bulgarian fruits and vegetables, J. Univ. Chem. Technol. Metallurgy, 2005, vol. 40, pp. 255–260.Google Scholar
  11. 11.
    Chang, C.C., Yang, M.H., Wen, H.M., and Chern, J.C., Estimation of total flavonoid content in propolis by two complementary colorimetric methods, J. Food Drug Anal., 2002, vol. 10, pp. 178–182.Google Scholar
  12. 12.
    Burits, M. and Bucar, F., Antioxidant activity of Nigella sativa essential oil, Phytother. Res., 2000, vol. 14, pp. 323–328.CrossRefGoogle Scholar
  13. 13.
    Boominathan, R. and Doran, P.M., Ni-induced oxidative stress in roots of the Ni hyperaccumulator, Alyssum bertolonii, New Phytol., 2002, vol. 156, pp. 205–215.CrossRefGoogle Scholar
  14. 14.
    Tripathi, D.K., Tripathi, A., Singh, S., Singh, Y., Vishwakarma, K., Yadav, G., Sharma, S., Singh, V.K., Mishra, R.K., Upadhyay, R.G., and Dubey, N.K., Uptake, accumulation and toxicity of silver nanoparticle in autotrophic plants, and heterotrophic microbes: a concentric review, Front. Microbiol., 2017, vol. 8: 7.Google Scholar
  15. 15.
    Hara, M., Oki, K., Hoshino, K., and Kuboi, T., Enhancement of anthocyanin biosynthesis by sugar in radish (Raphanus sativus) hypocotyl, Plant Sci., 2003, vol. 164, pp. 259–265.CrossRefGoogle Scholar
  16. 16.
    Dai, L.P., Xiong, Z.T., Huang, Y., and Li, M.J., Cadmium-induced changes in pigments, total phenolics, and phenylalanine ammonia-lyase activity in fronds of Azolla imbricate, Environ. Toxicol., 2006, vol. 21, pp. 505–512.CrossRefGoogle Scholar
  17. 17.
    Posmyk, M., Kontek, R., and Janas, K., Antioxidant enzymes activity and phenolic compounds content in red cabbage seedlings exposed to copper stress, Ecotoxicol. Environ. Saf., 2009, vol. 72, pp. 596–602.CrossRefGoogle Scholar
  18. 18.
    Syu, Y.Y., Hung, J.H., Chen, J.C., and Chuang, H.W., Impacts of size and shape of silver nanoparticles on Arabidopsis plant growth and gene expression, Plant Physiol. Biochem., 2014, vol. 83, pp. 57–64.CrossRefGoogle Scholar
  19. 19.
    Prakash, M., Nair, G., and Chung, I.M., Impact of copper oxide nanoparticles exposure on Arabidopsis thaliana growth, root system development, root lignification, and molecular level changes, Environ. Sci. Po-llut. Res. Int., 2014, vol. 21, pp. 12709–12722.CrossRefGoogle Scholar
  20. 20.
    Homaee, M.B. and Ehsanpour, A.A., Physiological and biochemical responses of potato (Solanum tuberosum) to silver nanoparticles and silver nitrate treatments under in vitro conditions, Ind. J. Plant Physiol., 2015, vol. 20, pp. 353–359.CrossRefGoogle Scholar
  21. 21.
    Thiruvengadam, M., Gurunathan, S., and Chung, I.M., Physiological, metabolic, and transcriptional effects of biologically-synthesized silver nanoparticles in turnip (Brassica rapa), Protoplasma, 2015, vol. 252, pp. 1031–1046.CrossRefGoogle Scholar
  22. 22.
    Atanassova, M., Georgieva, S., and Ivancheva, K., Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs, J. Univ. Chem. Technol. Metallurgy, 2011, vol. 46, pp. 81–88.Google Scholar
  23. 23.
    Krishnaraj, C., Jagan, E.G., Ramachandran, R., Abirami, S.M., Mohan, N., and Kalaichelvan, P.T., Effect of biologically synthesized silver nanoparticles on Bacopa monnieri (Linn.) Wettst. plant growth metabolism, Process Biochem., 2012, vol. 47, pp. 651–658.CrossRefGoogle Scholar
  24. 24.
    Miller, G., Suzuki, N., Ciftci-Yilmaz, S., and Mittler, R., Reactive oxygen species homeostasis and signalling during drought and salinity stresses, Plant Cell Environ., 2010, vol. 33, pp. 453–467.CrossRefGoogle Scholar
  25. 25.
    Zhang, F.Q., Wang, Y.S., Lou, Z.P., and Dong, J.D., Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plant seedlings (Kandelia candel and Bruguiera gymnorrhiza), Chemosphere, 2007, vol. 67, pp. 44–50.CrossRefGoogle Scholar
  26. 26.
    Geng, X.M., Liu, J., Lu, J.G., Hu, F.R., and Okubo, H., Effects of cold storage and different pulsing treatments on postharvest quality of cut OT lily “Mantissa” flowers, J. Fac. Agr., Kyushu Univ., 2009, vol. 54, pp. 41–45.Google Scholar
  27. 27.
    Dimkpa, C.O., McLean, J.E., Latta, D.E., Manangón, E., Britt, D.W., Johnson, W.P., Boyanov, M.I., and Anderson, A.J., CuO and ZnO nanoparticles: phytotoxicity, metal speciation, and induction of oxidative stress in sand-grown wheat, J. Nanoparticle Res., 2012, vol. 14, no. 9: 1125.CrossRefGoogle Scholar
  28. 28.
    Noreen, Z. and Ashraf, M., Changes in antioxidant enzymes and some key metabolites in some genetically diverse cultivars of radish (Raphanus sativus L.), Environ. Exp. Bot., 2009, vol. 67, pp. 395–402.CrossRefGoogle Scholar
  29. 29.
    Sreenivasulu, N., Grimm, B., Wobus, U., and Weschke, W., Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedlings of foxtail millet (Setaria italica), Physiol. Plant., 2000, vol. 109, pp. 435–442.CrossRefGoogle Scholar
  30. 30.
    Küçük, M., Kolaylι, S., Karaoğlu, Ş., Ulusoy, E., Baltacι, C., and Candan, F., Biological activities and chemical composition of three honeys of different types from Anatolia, Food Chem., 2007, vol. 100, pp. 526–534.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  1. 1.Department of Biology, Faculty of Science, Urmia UniversityUrmiaIran

Personalised recommendations