The ABA- and Stress-Induced Expression of the ArabidopsisthalianaAt4g0180 Gene Is Determined by the Cis-Elements Responsible for Binding the ABA-Dependent Trans-Factors
- 5 Downloads
In silico analysis of the promoter region of the At4g01870 gene of Arabidopsis thaliana (L.) Heynh. showed the presence of ABRE, W-box, RAV1-A, MYB, and LFY cis-elements in the sequence. These regulatory motifs bind the transcription factors involved in responses to abscisic acid (ABA) and stresses. Stable transgenic plants carrying the β-glucuronidase gene under the control of the 5'-deletion fragments of the At4g01870 promoter were obtained. According to the results of histochemical staining of transformants, gene expression was induced by abiotic stress and was most significant in the conductive tissues of the root, leaves, and sepals as well as in flowers. The study of At4g01870 gene expression by RT-PCR confirmed that the gene transcript content increased after the exposure of plants to a solution of NaCl or at 37°C and after ABA treatment; however, hypothermia almost unchanged the level of accumulation of the transcripts. Along with ABA, expression of the At4g01870 gene was induced by indolylacetic and salicylic acids and ethylene precursor 1-aminocyclopropane-1-carboxylic acid; it was hardly regulated by methyl jasmonate and inhibited by cytokinin. The TolB-like protein, encoded by the At4g01870 gene, functions as a type of platform, based on which protein complexes are assembled. Given the previously identified ABA-binding properties of the protein At4g01870 and the presence of the ABA-dependent cis-elements in the promoter of its coding gene, it can be assumed that the protein encoded by the At4g01870 gene allows to control the hormonal signals in the cell, providing a structural platform for the interaction of specific effector proteins, trans-factors and ion channels.
Keywords:Arabidopsis thaliana abscisic acid cis-elements regulation of gene expression At4g01870 gene
This study was supported by the Russian Science Foundation (project no. 14-14-00584).
COMPLIANCE WITH ETHICAL STANDARDS
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
- 1.Vishwakarma, K., Upadhyay, N., Kumar, N., Yadav, G., Singh, J., Mishra, R.K., Kumar, V., Verma, R., Upadhyay, R.G., Pandey, M., and Sharma, S., Abscisic acid signaling and abiotic stress tolerance in plants: a review on current knowledge and future prospects, Front. Plant Sci., 2017, vol. 8: 161.Google Scholar
- 7.Demidenko, A.V., Kudryakova, N.V., Karavaiko, N.N., Kazakov, A.S., Cherepneva, G.N., Shevchenko, G.V., Permyakov, S.E., Kulaeva, O.N., Oelmüller, R., and Kusnetsov, V.V., The ABA-binding protein AA1 of Lu-pinus luteus is involved in ABA-mediated responses, Russ. J. Plant Physiol., 2015, vol. 62, pp. 161–170.CrossRefGoogle Scholar
- 10.Kilian, J., Whitehead, D., Horak, J., Wanke, D., Weinl, S., Batistic, O., D’Angelo, C., Bornberg-Bauer, E., Kudla, J., and Harter, K., The AtGenE-xpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses, Plant J., 2007, vol. 50, pp. 347–363.CrossRefGoogle Scholar
- 13.Vitha, S., Benes, K., Phillips, J.P., and Gartland, K.M.A., Histochemical GUS analysis, Methods Mol. Biol., 1995, vol. 44, pp. 185–193.Google Scholar
- 14.Danilova, M.N., Kudryakova, N.V., Voronin, P.Yu., Oelmüller, R., Kusnetsov, V.V., and Kulaeva, O.N., Membrane receptors of cytokinin and their regulatory role in Arabidopsis thaliana plant response to photooxidative stress under conditions of water deficit, Russ. J. Plant Physiol., 2014, vol. 61, pp. 434–442.CrossRefGoogle Scholar
- 15.Yilmaz, A., Mejia-Guerra, M.K., Kurz, K., Liang, X., Welch, L., and Grotewold, E., AGRIS: Arabidopsis Gene Regulatory Information Server, an update, Nucleic Acids Res., 2011, vol. 39, database issue, pp. 1118–1122.Google Scholar
- 20.Lamb, R.S., Hill, T.A., Tan, Q.K.G., and Irish, V.F., Regulation of APETALA3 floral homeotic gene expression by meristem identity genes, Development, 2002, vol. 129, pp. 2079–2086.Google Scholar
- 22.Feng, C.Z., Chen, Y., Wang, C., Kong, Y.H., Wu, W.H., and Chen, Y.F., Arabidopsis RAV1 transcription factor, phosphorylated by SnRK2 kinases, regulates the expression of ABI3, ABI4, and ABI5 during seed germination and early seedling development, Plant J., 2014, vol. 80, pp. 654–668.CrossRefGoogle Scholar
- 27.Shang, Y., Yan, L., Liu, Z.Q., Cao, Z., Mei, C., Xin, Q., Wu, F.Q., Wang, X.F., Du, S.Y., Jiang, T., Zhang, X.F., Zhao, R., Sun, H.L., Liu, R., Yu, Y.T., et al., The Mg-chelatase H subunit of Arabidopsis antagonizes a group of WRKY transcription repressors to relieve ABA-responsive genes of inhibition, Plant Cell, 2010, vol. 22, pp. 1909–1935.CrossRefGoogle Scholar
- 28.Vinogradov, N.V., Danilova, M.V., Kudryakova, N.V., Kusnetsov, V.V., and Kulaeva, O.N., ABA-dependent regulation of the expression of AA1 homologs (abscisic acid activated 1) in A. thaliana mutants with impaired synthesis or transduction of ABA signal, Mater. Mezhd. nauch. konf. “Fiziologiya rastenii—teoreticheskaya osnova innovatsionnykh agro- i fitobiotekhnologii” (Proc. Int. Sci. Conf. “Plant Physiology—Theoretical Basis for Innovative Agro- and Phytobiotechnologies”), Kaliningrad, 2014, pp. 33–35.Google Scholar