The Expression Characteristics of Transcription Factors Regulated by Exogenous ABA in Winter Wheat (Triticum aestivum) under Cold Stress
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‘Dongnong Dongmai1 (Dn1)’ is a cultivar of wheat (Triticum aestivum L.) which may endure‒30°C in field with a reviving rate of 85%. ABA may enhance plant resistance. Aiming to reveal Dn1 coldresistant mechanism after exogenous ABA (exo-ABA) treated (control: non-ABA treated), some transcription factors (TFs) were selected and detected. Seedlings were prayed with exo-ABA at three-leaf stage, subsequently their tilling nodes were respectively sampled when it was at 5, 0,–10 and–25°C in field. Six TF genes (TabZIP1, TaWABI5, TaMYB1, TaMYB80, TaNAC2 and TaWRKY80) were selected and their expression at different low temperature was detected, the results showed that: in non-ABA treated tilling nodes, the expression of six TF genes all reached their peak level at–10°C; in exo-ABA treated tilling nodes, the expression of five TF genes (TabZIP1, TaWABI5, TaMYB80, TaNAC2 and TaWRKY80) reached their peak level at–25°C, and particularly the expression level of TabZIP1 was the highest in five TFs. In order to further defined the biological function of TabZIP1, the physical and chemical properties, protein secondary structure and the evolutionary tree was analyzed and predicted by some bioinformatics software or online program; TabZIP1 was localized in nuclear of tobacco (Nicotiana tabacum), which was observed by laser scanning confocal microscope (LSCM). This study initially exposed some TFs in Dn1 were upregulated to respond to the coldstress after exo-ABA treated, which indicated that a serious cold-responsive genes could be transcripted with the TFs assistance, and the results provided new theoretical basis to reveal the cold-reponsive mechanism of winter wheat.
KeywordsTriticum aestivum winter wheat exogenous ABA cold-stress transcription factors subcellular localization
winter wheat cv. Dongnong Dongmai1
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