Application of Methodological Analysis for Hydrocortisone Nanocapsulation in Biodegradable Polyester and MTT Assay
In recent decades, encapsulation of drugs into micro/nanoparticles of biodegradable polymers by different methods has been considered since they can gradually release drug over a particular time and reduce side effects. The present work is focused on nanocapsulation of hydrocortisone by biodegradable poly-ε- caprolactone using three methods (interfacial deposition, emulsion-diffusion and solvent displacement). The products were evaluated in terms of maximum adsorption, size distribution, zeta potential, efficiency (EE%), and were further characterized by Fourier transform infrared spectrometry and morphological studies. In vitro toxicity of this system was also studied. Depending on the physiochemical characteristics of a drug, the best method should be chosen to achieve an efficient entrapment of the drug without reducing its pharmacological activity. According to the obtained results, we compared these procedures and found that interfacial deposition gives more EE%, negative zeta potential, less particle size as well as suitable polydispersity indices. Furthermore, this study demonstrated that hydrocortisone did not show any toxic effect at lower concentrations in our formulations. In this respect, the development of a simple, safe and reproducible technique that allows an effective drug encapsulation can be a fundamental goal for nanotechnology.
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