Infrared Thermography Diagnostics of Subcutaneous Thermogenerators of Non-Shivering Thermogenesis
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Changes in skin temperature induced by the factors causing the activation of non-shivering thermogenesis (NST) were quantitatively described, using dynamic infrared thermography, in 8 physically active men (the mean age was 24.8 ± 4.0 years, body mass index (BMI) was 23.6 ± 0.44) at different body surface locations (the anterior and posterior parts of the neck, the supraclavicular fossae, the sternum, and the interscapular area). During the experiments, the subjects had to undergo, on different days, a glucose-tolerance test, they were locally exposed to cold (feet immersion in water at 0°C for 1 min) and had to perform a single breath-hold test, as well as the aerobic (Ramp) and anaerobic (Wingate) performance tests. The obtained results have shown the presence of thermogenerators, which can cause non-shivering thermogenesis in the human body in response to stimuli of sympathetic and stressogenic origin. A thermogenerator in this context is understood as a cluster of homogeneous cells located subcutaneously or in deeper-laying tissues characterized by elevated heat production whose flow of infrared radiation reaches the body’s surface and shapes a particular thermographic portrait. Deep individual differences have been identified between responses of thermogenerators to the same stimuli. These responses do not differ in synchronicity or intensity and, presumably, depend on a subject's adaptive experience, i.e., on the subject’s life conditions and other epigenetic factors. It has been shown that the thermogenerators located in the supraclavicular region and associated with the brown adipose tissue (BAT) have the highest sensitivity to the tested set of stimuli. A close functional connection has been identified between these thermogenerators and the thyroid gland. Some not at all trivial relationships have been detected between all the studied thermogenerators, and further studies are needed in this area. In particular, we cannot detect any similarities between maximal aerobic and maximal anaerobic exercises in terms of thermogenic response. The glucose response was isolated relative to other stimuli. The data obtained make us think not only about BAT but also about the role of other tissues in energy metabolism regulation. For instance, close attention should be paid to the muscle tissue which has uncoupling protein UCP3. Based on the results, we cannot make an unequivocal conclusion about the nature of investigated thermogenerators. Yet we hope that the widespread usage of non-invasive and safe thermography will allow us to accumulate scientific facts that are necessary to make differential diagnosis for various types of thermogenerators in the human body.
Keywords:infrared thermography skin temperature brown fat energy metabolism non-shivering thermogenesis muscles
We thank the volunteers participating in the experimental study for their fruitful cooperation; as well as R.S. Andreev, Cand. Sci. (Biol.), and А.V. Yakushkin for the fruitful discussion of methodical approaches and the obtained results, as well as O.I. Parfent’eva for her aid in the statistical analysis of the results.
The study has been supported within the research program of the Department of Physiology, Russian State University of Physical Education, Sports, Youth, and Tourism for 2015–2020 (Combined Plan for Research Studies, SCOLIPE, topic 03.00.12. Human Homeostatic Nonshivering Thermogenesis: Interaction between the Mechanisms of Optional Nonshivering Thermogenesis and Adaptation to Physical Loads), as well as with a financial support of the Center of Advanced Sports Technologies and National Teams, Moskomsport.
COMPLIANCE WITH ETHICAL STANDARDS
Conflict of interests. The authors declare the absence of obvious and potential conflicts of interests associated with the publication of this article.
Statement of compliance with standards of research involving humans as subjects. All studies have been conducted in line with the principles of biomedical ethics formulated under the Helsinki Declaration 1964 and its subsequent amendments and approved by the local bioethical committee at the Center of Advanced Sports Technologies and National Teams (Moskomsport ) (Moscow). All participants in the study gave their voluntary informed written consent signed after informing them about potential risks an advantages, as well as about the nature of the planned study.
- 2.Kolesov, S.N., Volovik, M.G., and Priluchnyi, M.A., Meditsinskoe teploradiovidenie: sovremennyi metodologicheskii podkhod (Medical Thermal Imaging: Modern Methods), Nizhny Novgorod: Nizhegorodsk. Nacuhno-Issled. Inst. Travmatol. Ortop., 2008.Google Scholar
- 7.Akimov, E.B., Andreev, R.S., Kalenov, Yu.N., et al., Possibilities of infrared thermography for identification of morphofunctional characteristics of a person (children and adults), Vestn. Mosk. Univ., Ser. 23: Antropol., 2016, no. 3, p. 49.Google Scholar
- 14.Khizhnyak, L.N., Khizhnyak, E.P., and Ivanitskii, G.R., Diagnostic capabilities of matrix infrared thermography: problems and prospects, Vestn. Nov. Med. Tekhnol., 2012, vol. 19, no. 4, p. 170.Google Scholar
- 15.Gatidis, S., Schmidt, H., Pfannenberg, C.A., et al., Is it possible to detect activated brown adipose tissue in humans using single-time- point infrared thermography under thermoneutral conditions? Impact of BMI and subcutaneous adipose tissue thickness, PLoS One, 2016, vol. 11, p. e0151152.CrossRefGoogle Scholar
- 17.Koksharova, E.O., Maiorov, A.Yu., Shestakova, M.V., et al., Metabolic features and therapeutic potential of brown and beige adipose tissue, Sakharnyi Diabet, 2014, no. 4, p. 5.Google Scholar
- 22.Kim, K., Huang, S., Fletcher, L.A., et al., Whole body and regional quantification of active human brown adipose tissue using 18F-FDG PET/CT, J. Vis. Exp., 2019, vol. 146. https://doi.org/10.3791/58469
- 26.Volkov, N.I., Melikhova, M.A., Oleinikov, V.I., and Tambovtseva, R.V., Obshchaya biokhimiya i biokhimiya fizicheskikh uprazhnenii: Uchebnoe posobie (General Biochemsitry and Biochemistry of Physical Exercises: Manual), Moscow: Ross. Gos. Univ. Fiz. Kul’t., Sporta, Molodehzi, Turizma, 2015, part 1.Google Scholar
- 28.De Matteis, R., Lucertini, F., Guescini, M., et al., Exercise as a new physiological stimulus for brown adipose tissue activity, Nutr. Metab. Cardiovasc., 2012, no. 6, p. 582.Google Scholar
- 29.Son’kin, V.D., Akimov, E.B., Andreev, R.S., et al., Brown adipose tissue participate in lactate utilization during muscular work, Proc. 2nd Int. Congr. on Sports Sciences Research and Technology Support (icSPORTS-2014), Setúbal, 2014, p. 97.Google Scholar
- 32.Akimov, E.B., Andreev, R.S., Kalenov, Yu.N., et al., The human thermal portrait and its relations with aerobic working capacity and the blood lactate level, Fiziol. Chel., 2010, vol. 36, no. 4, p. 89.Google Scholar
- 35.Nedergaard, J. and Cannon, B., Brown adipose tissue as a heat-producing thermoeffector, in Handbook of Clinical Neurology, Vol. 156: Thermoregulation: From Basic Neuroscience to Clinical Neurology, Romanovsky, A.A., Ed., Amsterdam: Elsevier, 2018, chap. 9. https://doi.org/10.1016/B978-0-444-63912-7.00009-6 Google Scholar
- 36.Jaksic, P.V., Grizelj, D., Livun, A., et al., Neck adipose tissue—tying ties in metabolic disorders, Horm. Mol. Biol. Clin. Invest., 2018, vol. 33, no. 2. https://doi.org/10.1515/hmbci-2017-0075
- 41.Astrup, A., Thermogenesis in human brown adipose tissue and skeletal muscle induced by sympathomimetic stimulation, Acta Endocrinol. Suppl., 1986, vol. 278, p. 1.Google Scholar
- 44.Gribova, N.V. and Sonkin, V.D., The development of brown adipose tissue in ontogenesis, in Morfofunktsional’nye osobennosti rastushchego orgnaizma (Morphofunctional Features of Growing Organism), Moscow: Akad. Pedagog. Nauk SSSR, 1974, p. 120.Google Scholar
- 47.van Marken Lichtenbelt, W.D. and Schrauwen, P., Implications of nonshivering thermogenesis for energy balance regulation in humans, Am. J. Physiol.: Regul., Integr. Comp. Physiol., 2011, vol. 301, p. R285.Google Scholar