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Human Physiology

, Volume 45, Issue 6, pp 693–700 | Cite as

Effects of Melatonin on the Oxygen Transport in Blood, Gas Transmitters, and Prooxidant–Antioxidant Balance in the Exercise

  • V. V. ZinchukEmail author
  • I. A. Poluyan
  • S. V. Hlutkin
Article

Abstract

We studied the effects of melatonin on the oxygen transport in blood and prooxidant−antioxidant balance in males aged from 18 to 21 years in submaximal physical exercise. The subjects received 3 mg of melatonin once a day for two months. As a result of melatonin administration, we observed a shift of oxyhemoglobin dissociation to the right, which results in reduced manifestation of oxidative stress after physical exercise. We found that the increased level of gas transmitters (nitrogen monoxide and hydrogen sulfide) after melatonin administration can influence oxygen transport in blood and prooxidant–antioxidant balance in exercise.

Keywords:

physical exercise melatonin oxygen lipid peroxidation antioxidants gas transmitters 

Notes

ACKNOWLEDGMENTS

We thank the staff of the research group for the study of the gas transmission function of blood in the research part of the Grodno State Medical University.

FUNDING

This study was supported by project no. 20 170 643.

COMPLIANCE WITH ETHICAL STANDARDS

Conflict of interests. The authors declare no obvious and potential conflicts of interest related to the publication of this article.

Statement of compliance with standards of research involving humans as subjects. All studies were conducted in accordance with the principles of biomedical ethics, formulated in the Helsinki Declaration of 1964 and its subsequent updates, and approved by the local bioethical committee of the Grodno State Medical University (Grodno, Belarus). Each research participant presented a voluntary written informed consent, signed by him after explaining to him the potential risks and benefits, as well as the nature of the upcoming research.

REFERENCES

  1. 1.
    Volkov, N.I., Kornienko, T.G., and Tambovtseva, R.V., Rates of breathing values and kinetics of respiration response in critical patterns of muscular activity in middle and long distance running, Hum. Physiol., 2014, vol. 40, no. 5, p. 542.CrossRefGoogle Scholar
  2. 2.
    Zinchuk, V.V. and Zhadko, D.D., The effect of a sauna on blood oxygen transport and the prooxidant-antioxidant balance in untrained subjects, Hum. Physiol., 2012, vol. 38, no. 5, p. 548.CrossRefGoogle Scholar
  3. 3.
    Popichev, M.I., Konoshenko, S.V., Tolkacheva, N.V., et al., Intraerythrocyte metabolism and affinity of hemoglobin for oxygen in athletes of various qualifications exposed to intense physical load, Fiziol. Chel., 1999, vol. 25, no. 6, p. 123.Google Scholar
  4. 4.
    Reiter, R.J., Mayo, J.C., Tan, D.X., et al., Melatonin as an antioxidant: under promises but over delivers, J. Pineal. Res., 2016, vol. 61, no. 3. P. 253.CrossRefGoogle Scholar
  5. 5.
    Arushanyan, E.B. and Beier, E.V., Melatonin and blood system, Eksp. Klin. Farmakol., 2006, vol. 69, no. 3, p. 74.Google Scholar
  6. 6.
    Zinchuk, V.V., Glutkin, S.V., Shul’ga, E.V., and Gulyai, I.E., The effect of melatonin on oxygen-dependent processes, Eksp. Klin. Farmakol., 2013, vol. 76, no. 2, p. 32.PubMedGoogle Scholar
  7. 7.
    Karpman, V.L., Belotserkovskii, Z.B., and Gudkov, I.A., Testirovanie v sportivnoi meditsine (Examination in Sports Medicine), Moscow: Fizkul’tura i Sport, 1988.Google Scholar
  8. 8.
    Severinghaus, J.W., Blood gas calculator, J. Appl. Physiol., 1966, vol. 21, no. 5, p. 1108.CrossRefGoogle Scholar
  9. 9.
    Kamyshnikov, V.S., Spravochnik po kliniko-biokhimicheskoi laboratornoi diagnostike (Handbook on Clinical-Biochemical Laboratory Diagnostics), Minsk: Belarus’, 2002, vol. 1.Google Scholar
  10. 10.
    Rice-Evans, C.A., Diplock, A.T., and Symons, M.C.R., Laboratory Techniques in Biochemistry and Molecular Biology: Techniques in Free Radical Research, London: Elsevier, 1991.Google Scholar
  11. 11.
    Sedlak, J. and Lindsay, R.N., Estimation of total, protein-bound, and protein sulfhydryl groups in tissue with Ellman’s reagent, Anal. Biochem., 1968, vol. 25, no. 1, p. 192.CrossRefGoogle Scholar
  12. 12.
    Taylor, S.L., Lamden, M.P., and Tappel, A.L., Sensitive fluorometric method for tissue tocopherol analysis, Lipids, 1976, vol. 11, no. 7, p. 530.CrossRefGoogle Scholar
  13. 13.
    Ragino, Yu.I., Voevoda, M.I., Kashtanova, E.V., et al., The use of new biochemical methods for evaluation of the oxidative-antioxidant potential of low-density lipoproteins, Klin. Lab. Diagn., 2005, no. 4, p. 11.Google Scholar
  14. 14.
    Aruoma, O.I. and Cuppett, S.L., Antioxidant Methodology: In Vivo and in Vitro Concepts, New York: Am. Oil Chem. Soc., 1997.Google Scholar
  15. 15.
    Bryan, N.S. and Grisham, M.B., Methods to detect nitric oxide and its metabolites in biological samples, Free Radicals Biol. Med., 2007, vol. 43, no. 5, p. 645.CrossRefGoogle Scholar
  16. 16.
    Norris, E.J., Culberson, C.R., Narasimhan, S., and Clemens, M.G., The liver as a central regulator of hydrogen sulfide, Shock, 2011, vol. 36, no. 3. P. 242.CrossRefGoogle Scholar
  17. 17.
    Kapilevich, L.V., Kabachkova, A.V., Zakharova, A.N., et al., Skeletal muscle secretory function: production mechanisms and physiological effects of myokins, Usp. Fiziol. Nauk, 2016, vol. 47, no. 2, p. 7.PubMedGoogle Scholar
  18. 18.
    Zinchuk, V.V., Lepeev, V.O., and Gulyai, I.E., The participation of gas transmitters in the modification of the oxygen transport function of the blood in magnetic field, Ross. Fiziol. Zh. im. I.M. Sechenova, 2016, vol. 102, no. 10, p. 1176.PubMedGoogle Scholar
  19. 19.
    Wagner, P.D., Wagner, H.E., Groves, B.M., et al., Hemoglobin P50 during a simulated ascent of Mt. Everest, Operation Everest II, High Alt. Med. Biol., 2007, vol. 8, no. 1. P. 32.CrossRefGoogle Scholar
  20. 20.
    Sukmanskii, O.I. and Reutov, V.P., Gas transmitters: physiological role and participation in the pathogenesis of diseases, Usp. Fiziol. Nauk, 2016, vol. 47, no. 3, p. 30.Google Scholar
  21. 21.
    Yang, J., Li, H., Ochs, T., et al., Erythrocytic hydrogen sulfide production is increased in children with vasovagal syncope, J. Pediatry, 2015, vol. 166, no. 4, p. 965.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  • V. V. Zinchuk
    • 1
    Email author
  • I. A. Poluyan
    • 1
  • S. V. Hlutkin
    • 1
  1. 1.Grodno State Medical UniversityGrodnoBelarus

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