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Caenibaculum baiyandianus gen. nov., sp. nov., a New Bacterium Isolated from Sewage Sediment of Baiyangdian

Abstract

An aerobic, gram-negative-staining, motile and rod-shaped bacterium, designated strain 3-BNT, was isolated from sewage sediment collected from Baiyangdian, located in Xiongan New Area of China. This heterotrophic bacterium grows on glucose, yeast extract and a limited range of defined organic acids including lactic and propionic acids. The major isoprenoid quinone is ubiquinone Q-8. Major polar lipids are phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidyl glycerol (DPG) and three unidentified glycolipids. The major fatty acids are C16:0 (30.9%), C18:1ω7c (18.3%), C17:0 cyclo (16.1%) and C14:0 (7.9%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 3-BNT belongs to the family Burkholderiaceae, and its closest phylogenetic neighbors are members of the genera Burkholderia and Caballeronia. However, strain 3-BNT did not make a coherent clade with members of the currently recognized genera. Based on phylogenetic and chemotaxonomic characteristics of this bacterium, a novel genus and species, Caenibaculum baiyandianus gen. nov., sp. nov., is proposed. The type strain is 3-BNT (=ACCC 60082T=KCTC 62431T).

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REFERENCES

  1. 1

    Buck, J.D., Nonstaining (KOH) method for determination of gram reactions of marine bacteria, Appl. Environ. Microbiol., 1982, vol. 44, no. 4, pp. 992‒993.

  2. 2

    Chen, W.M., Xie, P.B., Young, C.C., and Sheu, S.Y., Formosimonas limnophila gen. nov., sp. nov., a new member of the family Burkholderiaceae isolated from a freshwater lake, Int. J. Syst. Evol. Microbiol., 2017, vol. 67, no. 1, pp. 17‒24.

  3. 3

    Dobritsa, A.P. and Samadpour, M., Transfer of eleven species of the genus Burkholderia to the genus Paraburkholderia and proposal of Caballeronia gen. nov. to accommodate twelve species of the genera Burkholderia and Paraburkholderia,Int. J. Syst. Evol. Microbiol., 2016, vol. 66, no. 8, pp. 2836−2846.

  4. 4

    Felsenstein, J., Confidence limits on phylogenies: an approach using the bootstrap, Evolution, 1985, vol. 39, no. 4, pp. 783‒791.

  5. 5

    Garrity, G.M., Bell, J.A., and Lilburn, T., List of new names and new combinations previously effectively, but not validly, published, Int. J. Syst. Evol. Microbiol., 2006, vol. 56, no. 1, pp. 1‒6.

  6. 6

    Gordon, R.E. and Mihm, J.M., A comparative study of some strains received as nocardiae, J. Bacteriol., 1957, vol. 73, no. 1, pp. 15‒27.

  7. 7

    Kim, S.J., Ahn, J.H., Weon, H.Y., Hong, S.B., Seok, S.J., and Kwon, S.W., Parasegetibacter terrae sp. nov., isolated from paddy soil and emended description of the genus Parasegetibacter,Int. J. Syst. Evol. Microbiol., 2015, vol. 65, no. 1, pp. 113‒116.

  8. 8

    Kumar, S., Stecher, G., and Tamura, K., MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets, Mol. Biol. Evol., 2016, vol. 33, no. 7, pp. 1870‒1874.

  9. 9

    List of new names and new combinations previously effectively, but not validly, published, Int. J. Syst. Evol. Microbiol., 2006, vol. 56, no. 1, pp. 1‒6.

  10. 10

    Mesbah, M., Premachandran, U., and Whitman, W.B., Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography, Int. J. Syst. Bacteriol., 1989, vol. 39, no. 2, pp. 159‒167.

  11. 11

    Minnikin, D.E., O’Donnell, A.G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A., and Parlett, J.H., An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids, J. Microbiol. Methods, 1984, vol. 2, no. 5, pp. 233‒241.

  12. 12

    Nguyen, T.M., Lee, H., and Kim, J., Selective isolation of actinobacteria showing antibacterial activity against Paenibacillus larvae from soil samples collected in South Korea, J. Apiculture, 2013, vol. 28, no. 4, pp. 265‒272.

  13. 13

    Ohshima, S., Sato, Y., Fujimura, R., Takashima, Y., Hamada, M., Nishizawa, T., Narisawa, K., and Ohta, H., Mycoavidus cysteinexigens gen. nov., sp. nov., an endohyphal bacterium isolated from a soil isolate of the fungus Mortierella elongata,Int. J. Syst. Evol. Microbiol., 2016, vol. 66, no. 5, p. 2052.

  14. 14

    Saitou, N. and Nei, M., The neighbor-joining method: a new method for reconstructing phylogenetic trees, Mol. Bi-ol. Evol., 1987, vol. 4, no. 4, pp. 406‒425.

  15. 15

    Sasser, M., Identification of bacteria by gas chromatography of cellular fatty acids, Midi Technical Note, 2001, vol. 101.

  16. 16

    Sawana, A., Adeolu, M., and Gupta, R.S., Molecular signatures and phylogenomic analysis of the genus Burkholderia: proposal for division of this genus into the emended genus Burkholderia containing pathogenic organisms and a new genus Paraburkholderia gen. nov. harboring environmental species, Frontiers in Genetics, 2014, vol. 5, p. 429.

  17. 17

    Vandamme, P. and Eberl, L., Burkholderia, in Bergey’s Manual of Systematics of Archaea and Bacteria, 2018, pp. 1−45.

  18. 18

    Vaz-Moreira, I., Narciso-Da-Rocha, C., De, B.E., Vandamme, P., Silva Ferreira, A.C., Lobo-Da-Cunha, A., Nunes, O.C., and Manaia, C.M., Hydromonas duriensis gen. nov., sp. nov., isolated from freshwater, Int. J. Syst. Evol. Microbiol., 2015, vol. 65, no. 11, pp. 4134‒4139.

  19. 19

    Yoon, S.H., Ha, S.M., Kwon, S., Lim, J., Kim, Y., Seo, H., and Chun, J., Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies, Int. J. Syst. Evol. Microbiol., 2017, vol. 67, no. 5, pp. 1613‒1617.

  20. 20

    Zhang, X., Ren, K., Du, J., Liu, H., and Zhang, L., Glycomyces artemisiae sp. nov., an endophytic actinomycete isolated from the roots of Artemisia argyi,Int. J. Syst. Evol. Microbiol., 2014, vol. 64, no. 10, pp. 3492‒3495.

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Funding

This work was supported by the National Natural Science Foundation of China (grant no. 31270053), the Natural Science Foundation of Hebei Province, China C2014201141) and Key Bioengineering Discipline of Hebei Province (1050-5030023).

Author information

Correspondence to L. Zhang or X. Zhang.

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Conflicts of interest. The authors declare that there are no conflicts of interest.

Informed consent. Informed consent was obtained from all individual participants included in the study.

AUTHOR CONTRIBUTION

Yuhui Wang wrote the manuscript; Yuhui Wang, TianTian, Xiaojin Li, Lingjie Tang, Yunqi Li, Jinhua Zhang and Hongpeng Wang performed research; Xiumin Zhang and Liping Zhang designed experiments; Xiumin Zhang and Liping analyzed data.

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Wang, Y., Tian, T., Li, X. et al. Caenibaculum baiyandianus gen. nov., sp. nov., a New Bacterium Isolated from Sewage Sediment of Baiyangdian. Microbiology 89, 122–128 (2020). https://doi.org/10.1134/S0026261720010166

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Keyword:

  • Baiyangdian
  • Xiongan New Area
  • Burkholderiaceae
  • Caenibaculum baiyandianus gen. nov.
  • sp. nov.