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Ancylobacter lacus sp. nov. and Ancylobacter plantiphilus sp. nov., Novel Aerobic Facultative Methylotrophic Bacteria

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Abstract

Novel aerobic facultatively methylotrophic bacteria were isolated from the water of a freshwater lake (strain F30LT), soil sample of rhizosphere of white clover Trifolium repens L. (strain 1TCT), and rhizosphere of carrot Daucus carota L. (strain Dau2). The isolates were gram-negative, non-spore-forming, nonmotile, pleomorphic/rod-shaped cells, propagating by binary fission; they were facultative methylotrophs, using a broad range of polycarbon compounds, apart from methanol. Optimum growth occurred at 25−29 °C and pH 7.0‒7.5, 0.5 vol % СН3OH, and 0.05 wt % NaCl. The strains employ the ribulose bisphosphate pathway for C1 assimilation. The predominant phospholipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. In the whole-cell fatty acid pattern of strains, C18:1 ω7c, C19: 0 ω8c cyclo, C16:0 and С18:0 acids were predominant. The major ubiquinone was Q-10. According to the 16S rRNA gene sequencing, strain F30LT exhibited high similarity to Ancylobacter dichloromethanicus DM16T (97.8%), while strains 1ТСT and Dau2 were closely related to A. oerskovii DSM 18746T (98.6%). The 16S rRNA gene sequence similarity between strains 1TCT and Dau2 was 99.4%. DNA–DNA hybridization of the strain F30LT with A. dichloromethanicus DM16T revealed only 22% homology; homology for strain 1ТСT and A. oerskovii DSM 18746T was 27%, while DNA‒DNA homology between strains 1TCT and Dau2 was 77%. Based on the data obtained, strain F30LT was identified as a new species within the genus Ancylobacter, Ancylobacter lacus sp. nov. (VKM В-3280Т = DSM 106439T). Strains 1ТСТ and Dau2 (=ВКМ В-3227 = CCUG71988) represent a novel species of the same genus, for which the name Ancylobacterplantiphilus sp. nov. is proposed, with the type strain 1ТСТ (=VKM В-3219Т = DSM 106438T).

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Author information

Correspondence to N. V. Doronina.

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COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

AUTHORS’ CONTRIBUTIONS

A.A. Chemodurova, E.N. Kaparullina, Cand. Sci. (Biol.)—strain identification by the methods of polyphase taxonomy; A.A. Machulin, Cand. Sci. (Biol.)—microscopy; Dr. C. Spröer, Dr. Lang E.—DNA‒DNA hybridization and analysis of fatty acid composition of cells; N.V. Doronina, D. Sci. (Biol.)—isolation of pure cultures and analysis of enzyme activities. The authors participated in preparing the publication.

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Translated by E. Makeeva

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Chemodurova, A.A., Kaparullina, E.N., Machulin, A.V. et al. Ancylobacter lacus sp. nov. and Ancylobacter plantiphilus sp. nov., Novel Aerobic Facultative Methylotrophic Bacteria. Microbiology 89, 35–43 (2020). https://doi.org/10.1134/S0026261720010051

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Keywords:

  • Ancylobacter lacus sp. nov.
  • Ancylobacter plantiphilus sp. nov.
  • methylotrophic bacteria
  • new species