, Volume 87, Issue 6, pp 848–856 | Cite as

Anti-Oxidative Response of Cyanobacterium Anabaena sp. strain PCC 7120 to Arsenite (As(III))

  • U. DhuldhajEmail author
  • U. Pandya
  • S. SinghEmail author


Arsenite (As(III)) induced changes were investigated in the diazotrophic cyanobacterium Anabaena PCC 7120. The cells of Anabaena sp. strain PCC 7120 exposed to As(III) produced H2O2 and its production increased with increase in As(III) concentration. As a part of resistant mechanism and also to counteract the deleterious effect of H2O2, the cells of Anabaena sp. strain PCC 7120 produced more ascorbate peroxidase (APx) whose level also increased in response to As(III) concentrations. The increase in APx activity was directly proportional to the increase in H2O2 production and maximum APx activity was recorded at 40 ppm of As(III). In contrast, glutathione reductase (GR) activity decreases with increase in As(III) concentrations and attained its minimum level at 40 ppm of As(III). Lipid peroxidation increased with increase in As(III) concentration and maximum peroxidation (which was about two fold higher than that of the untreated control cells) was recorded at 40 ppm of As(III). Exposure of the Anabaena sp. strain PCC 7120cells to As(III) has also resulted in a significant increase in ascorbate and dehydro-ascorbate contents which was about 6.44 and 1.97 fold higher than that of the untreated control cells, respectively at 40 ppm of As(III).


antioxidant peroxidase glutathione reductase peroxidation 


  1. 1.
    Aebi, H. Catalase, in Methods in Enzymology, Packer, L., Ed., Orlando: Academic, 1984, vol. 105, pp. 121–126.Google Scholar
  2. 2.
    Bartosz, G., Oxidative stress in plants, Acta Physiol., Plant., 1997, vol. 19, pp. 47–64.CrossRefGoogle Scholar
  3. 3.
    Bhattacharjee, H., Mukhopadhyay, R., Thiyagarajan, S., and Rosen, B.P., Aquaglyceroporin: ancient channel for metalloids, J. Biol., 2008. vol. 7, pp. 33–35.CrossRefGoogle Scholar
  4. 4.
    Bhattacharya, P. and Pal, R., Response of cyanobacteria to arsenic toxicity, J. Appl. Phycol., 2011, vol. 23, pp. 293–299.CrossRefGoogle Scholar
  5. 5.
    Castillo, F.I., Penel, I., and Greppin, H., Peroxidase release induced by ozone in Sedum album leaves, Plant Physiol., 1984, vol. 74, pp. 846–851.CrossRefGoogle Scholar
  6. 6.
    Cerutti, P.A., Prooxidant states and tumor promotion, Science, 1985, vol. 227, pp. 375–381.CrossRefGoogle Scholar
  7. 7.
    Gebel, T. Confounding variables in the environmental toxicology of arsenic, Toxicol., 2000, vol. 144, pp. 155–162.CrossRefGoogle Scholar
  8. 8.
    Giannopolitis, C. and Ries, N. Superoxide dismutase I: occurrence in higher plants, Plant Physiol., 1977, vol. 59, pp. 309–314.CrossRefGoogle Scholar
  9. 9.
    Gratão, P.L., Polle, A., Lea, P.J., and Azevedo, R.A., Making the life of heavy metal-stress plants a little easier, Funct. Plant Biol., 2005, vol. 32, pp. 481–494.CrossRefGoogle Scholar
  10. 10.
    Hepler, P.K. and Wayne, R.O., Calcium and plant development, Annu. Rev. Plant Physiol., 1985, vol. 36, pp. 397–439.CrossRefGoogle Scholar
  11. 11.
    Hussein, K.A. and Joo, J.H., Heavy metal resistance of bacteria and its impact on the production of antioxidant enzymes, African J. Microbiol. Res., 2013, vol. 7, no. 20, pp. 288–2296.Google Scholar
  12. 12.
    Kenney, L. and Kaplan, J.H., Arsenate substitutes for phosphate in the human red cell sodium pump and anion exchanger, J. Biol. Chem., 1988, vol. 263, pp. 7954–7960.Google Scholar
  13. 13.
    Kwon, S.I. and Anderson, A.J., Catalase activities of Phanerochaete chrysosporium are not coordinately produced with ligninolytic metabolism: catalase from a white-rot fungus, Curr. Microbiol., 2001, vol. 42, pp. 8–11.CrossRefGoogle Scholar
  14. 14.
    Lenartova, V., Holovska, K., and Javorsky, P., The influence of mercury on the antioxidant enzyme activity of rumen bacteria Streptococcus bovis and Selenomonas ruminantium, FEMS Microbiol Ecol., 1998, vol. 27, pp. 319–325.CrossRefGoogle Scholar
  15. 15.
    Liu, J., Chen, H., Miller, D.S., Sauvedra, J.E., Keefer, L.K., Johnson, D.R., Klaassen, C.D., and Waalkes, M.P., Overexpression of glutathione s-transferase II and multi-drug resistance transport proteins is associated with acquired tolerance to inorganic arsenic, Mol. Pharmacol., 2001, vol. 60, pp. 302–309.CrossRefGoogle Scholar
  16. 16.
    Lurie, S., Antioxidants, in Postharvest Oxidative Stress in Horticultural Crops, Hodges, D.M., Ed., New York: Food Products Press, 2003, pp. 131–150.Google Scholar
  17. 17.
    Mascher, R., Lippman, B., Holiinger, S., and Bergmann, H., Arsenate toxicity: effects on oxidative stress response molecules and enzymes in red clover plants, Plant Sci., 2002, vol. 63, pp. 961–969.CrossRefGoogle Scholar
  18. 18.
    Montillet, J.L., Chamnongpol, S., Rustérucci, C., Dat, J., Van de Cotte, B., Agnel, J.-P., Battesti, C., Inzé, D., Van Breusegem, F., and Triantaphylidès, C., Fatty acid hydroperoxides and H2O2 in the execution of hypersensitive cell death in tobacco leaves, Plant Physiol., 2005, vol. 138, pp. 1516–1526.CrossRefGoogle Scholar
  19. 19.
    Mylona, P.V., Polidoros, A.N., and Scandalios, J.G., Modulation of antioxidant response by arsenic in maize, Free Rad. Biol. Med., 1998, vol. 25, no. 4/5, pp. 576–585.CrossRefGoogle Scholar
  20. 20.
    Nakano, Y. and Asada, K., Hydrogen peroxide is scavenged by ascorbate specific peroxide in spinach chloroplasts, Plant Cell Physiol., 1981, vol. 22, pp. 867–880.Google Scholar
  21. 21.
    Okamura, M., An improved method for determination of L-ascorbic acid and L-dehydroascorbic acid in blood plasma, Clin. Chem. Acta., 1980, vol. 103, pp. 259–268.CrossRefGoogle Scholar
  22. 22.
    Ohkawa, H., Ohishi, N., and Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Anal. Biochem., 1979, vol. 95, pp. 351–358.CrossRefGoogle Scholar
  23. 23.
    Pnueli, L., Liang, H., Rozenberg, M., and Mittler, R. Growth suppression, altered stomatal responses and augmented induction of heat shock proteins in cytosolic ascorbate peroxidase (Apx1)-deficient Arabidopsis plants, Plant J., 2003, vol. 34, pp. 187–203.CrossRefGoogle Scholar
  24. 24.
    Rai, A.K., Cyanobacterial Nitrogen Metabolism and Environmental Biotechnology, New Dehli: Narosa, 1997, pp. 73–87.Google Scholar
  25. 25.
    Rensing, C. and Rosen, B., Heavy metals cycle (arsenic, mercury, selenium, others), in Encyclopedia of Microbiology, Schaeter, M., Ed., Oxford: Elsevier, 2009, pp. 205–219.Google Scholar
  26. 26.
    Rippka, R., Derulles, J., Waterbury, J.B., Herdman, M., and Stanier, R.Y., Generic assignments, strain histories and properties of pure cultures of cyanobacteria, J. Gen. Microbiol., 1979, vol. 111, pp. 1–61.Google Scholar
  27. 27.
    Rosen, B.P., Biochemistry of arsenic detoxification, FEBS Lett., 2002, vol. 529, pp. 86–92.CrossRefGoogle Scholar
  28. 28.
    Santos, C., Gaspar, C.A., Branco-Price, C., Teixeira, A., and Ferreira, R.B., Exposure of Lemna minor to arsenite: expression levels of components and intermediates of the ubiquitin/proteasome pathway, Plant Cell Physiol., 2006, vol. 47, pp. 1262–1273.CrossRefGoogle Scholar
  29. 29.
    Schaedle, M. and Bassham, J.A., Chloroplast glutathione reductase, Plant Physiol., 1977, vol. 59, pp. 1011–1012.CrossRefGoogle Scholar
  30. 30.
    Schmoger, M.E.V., Oven, M., and Grill, E., Detoxification of arsenic by phytochelatins in plants, Plant Physiol., 2000, vol. 122, pp. 793–802.CrossRefGoogle Scholar
  31. 31.
    Singh, H.P., Batish, D.R., Kohlo, R.K., and Arora, K., Arsenic-induced root growth inhibition in mungbean (Phaseolus aureus Roxb.) is due to oxidative stress resulting from enhanced lipid peroxidation, Plant Growth Regul., 2007, vol. 53, pp. 65–73.CrossRefGoogle Scholar
  32. 32.
    Smedley, P.L. and Kinniburgh, D.G., A review of the source, behaviour and distribution of arsenic in natural waters, Appl. Geochem., 2002, vol. 17, pp. 517–568.CrossRefGoogle Scholar
  33. 33.
    Smirnoff, N., The role of active oxygen in the response of plants to water deficit and desiccation, New Phytol., 1993, vol. 125, pp. 27–58.CrossRefGoogle Scholar
  34. 34.
    Srivastava, A.K., Bhargava, P., and Rai, L.C., Differential response of antioxidative defense system of Anabaena doliolum under arsenite and arsenate stress, J. Basic Microbiol., 2009, vol. 49, pp. S63–S72.CrossRefGoogle Scholar
  35. 35.
    Srivastava, A.K., Bhargava, P., Mishra, Y., Shukla, B., and Rai, L.C., Effect of pretreatment of salt, copper and temperature on ultra-violet-B-induced antioxidants in diazotrophic cyanobacterium Anabaena doliolum, J. Basic Microbiol., 2006, vol. 46, pp. 135–144.CrossRefGoogle Scholar
  36. 36.
    Stoeva, N., Berova, M., and Zlatev, Z., Effect of arsenic on some physiological parameters in bean plants, Biol. Plant., 2005, vol. 49, pp. 293–296.CrossRefGoogle Scholar
  37. 37.
    Sundaram, S. and Soumya, K.K., Study of physiological and biochemical alterations in cyanobacterium under organic stress, Am. J. Plant. Physiol., 2011, vol. 6, pp. 1–16.CrossRefGoogle Scholar
  38. 38.
    Thiel, T., Phosphate transport and arsenate resistance in the cyanobacterium Anabaena variabilis, J. Bacteriol., 1988, vol. 170, pp. 1143–1147.CrossRefGoogle Scholar
  39. 39.
    Thordal-Christensen, H., Zhang, Z, Wei, Y., and Collinge, D.B., Subcellular localization of H2O2 in plants, H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction, The Plant J., 1997, vol. 11, pp. 1187–1192.CrossRefGoogle Scholar
  40. 40.
    Turpeinen, R., Interactions between metals, microbes and plants: bioremediation of arsenic and lead contaminated soils, MSc Dissertation in Environmental Ecology, Fac. Sci., Univ. Helsinki, 2002.Google Scholar
  41. 41.
    Velikova, V., Yordanov, I., and Edreva, A., Oxidative stress and some antioxidant systems in acid rain-treated bean plants, protective role of exogenous polyamines, Plant Sci., 2000, vol.151, pp. 59–66.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  1. 1.School of Life Sciences, Swami Ramanand Teerth Marathwada UniversityNandedIndia
  2. 2.Department of Microbiology, Samarpan Science and Commerce College, KH-7 Circle, Sector 28, Nr. Government Engineering CollegeGandhinagarIndia
  3. 3.Department of Botany, Banaras Hindu University, VaranasiIndia

Personalised recommendations