Biochemistry (Moscow)

, Volume 84, Issue 11, pp 1375–1389 | Cite as

Conditioned Medium of Mesenchymal Stromal Cells: A New Class of Therapeutics

  • N. V. BogatchevaEmail author
  • M. E. Coleman


Mesenchymal stromal cell (MSCs) represent a class of biologics with the prospects for employment as immunomodulatory, tissue-protective, and regenerative therapeutics. In parallel with cellular therapy, cell-free therapy based on MSC-secreted bioactive factors is being actively developed. MSCs secrete a variety of protein, peptide, RNA, and lipid mediators which can be concentrated, frozen, or even lyophilized without loss of activity, which gives them a certain advantage over cellular products requiring liquid nitrogen storage and infrastructure to revive frozen cells. This review (i) describes currently conducted clinical trials of cell-free products containing MSC secretome; (ii) summarizes main approaches to the generation and characterization of conditioned media concentrates and extracellular vesicle isolates; (iii) analyzes a variety of preclinical studies where effectiveness of secretome products has been shown; and (iv) summarizes current knowledge about secretome bioactive components obtained by analysis of in vivo models testing the therapeutic potential of the MSC secretome.


MSC secretome conditioned media extracellular vesicles exosomes 



mesenchymal stromal cells isolated from bone marrow, adipose tissue, dental pulp, and umbilical cord


conditioned medium


extracellular vesicles


Good Manufacturing Practices


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This work was partially supported by the National Institute of Diabetes, Digestive and Kidney Diseases (R41 1R41DK115317 grant to Dr. Coleman).


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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  1. 1.Division of Pulmonary and Critical Care, Department of MedicineIndiana University School of Medicine, IUPUIIndianapolisUSA
  2. 2.Theratome Bio, Inc.IndianapolisUSA

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