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Biochemistry (Moscow)

, Volume 84, Issue 9, pp 1065–1073 | Cite as

Comparison of State Transitions of the Photosynthetic Antennae in Arabidopsis and Barley Plants upon Illumination with Light of Various Intensity

  • D. V. Vetoshkina
  • M. A. Kozuleva
  • V. V. Terentyev
  • E. M. Zhurikova
  • M. M. Borisova-Mubarakshina
  • B. N. IvanovEmail author
Article
  • 7 Downloads

Abstract

Changes in the light energy distribution between the photosystems 1 and 2 (PS1 and PS2, respectively) due to the reversible migration of a part of the light-harvesting complex (LHC2) between the photosystems (state transitions, ST) have been studied in leaves of barley (Hordeum vulgare) and Arabidopsis thaliana plants upon short-term illumination with light of various intensity that excited predominantly PS2. Changes in the ratio of fluorescence maxima at 745 and 685 nm in the low-temperature (77 K) fluorescence spectrum of chlorophyll a (Chl a) characterizing energy absorption by the PS1 and PS2, respectively, were insufficient for revealing the differences in the STs in barley and Arabidopsis plants at various light intensities, because they were not associated with STs at high-intensity illumination. Light-induced accumulation of the LHC2 phosphorylated proteins Lhcb1 and Lhcb2 involved in the relocation of a part of the LHC2 from PS2 to PS1 in the leaves of both plants decreased with the increase in the light intensity and was more pronounced in barley than in Arabidopsis at the same light intensity. Relaxation of the non-photochemical quenching (NPQ) of Chl a fluorescence after illumination corresponding to the return of the part of LHC2 from PS1 to PS2 was observed in barley leaves in a wider range of increasing light intensities than in Arabidopsis leaves. The differences in the accumulation of phosphorylated Lhcb1 and Lhcb2, as well as in the parameters of NPQ relaxation after illumination, revealed that STs in barley leaves could occur not only at low- but also at high-intensity light, when it is absent in Arabidopsis leaves.

Keywords

Arabidopsis barley photosynthesis phosphorylation of antenna proteins chlorophyll a fluorescence 

Abbreviations

At-WT and At-stn7

wild-type and STN7 kinase knockout Arabidopsis plants, respectively

Chl

chlorophyll

Hv-NaF/Hv-H2O

barley leaves incubated in NaF solution or water, respectively

LHC

light-harvesting complex

NPQ

non-photochemical fluorescence quenching

PETC

photosynthetic electron transport chain

PS1(2)

photosystem 1(2)

S1(2)

state 1(2)

ST

state transition

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Notes

Acknowledgements

The authors are grateful to Prof. Dario Leister (Ludwig-Maximilians-Universität, München) for providing seeds of Arabidopsis mutant plants lacking STN7 kinase.

Funding. This work was supported by the Russian Science Foundation (projects 17-14-01371 and 17-76-10058).

Ethical approval. This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • D. V. Vetoshkina
    • 1
  • M. A. Kozuleva
    • 1
  • V. V. Terentyev
    • 1
  • E. M. Zhurikova
    • 1
  • M. M. Borisova-Mubarakshina
    • 1
  • B. N. Ivanov
    • 1
    Email author
  1. 1.Institute of Basic Biological Problems, Pushchino Scientific Center for Biological ResearchRussian Academy of SciencesPushchino, Moscow RegionRussia

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