Biochemistry (Moscow)

, Volume 84, Issue 8, pp 963–977 | Cite as

Analysis of Protein Synthesis in Cucumber Leaves after Inoculation with Corynespora cassiicola: A Proteomic Approach

  • G. Yu
  • Y. Yu
  • H. FanEmail author
  • D. Zhang
  • N. Cui
  • X. Wang
  • S. Jia
  • Y. Yang
  • J. Zhao


Cucumber target leaf spot (TLS) disease caused by Corynespora cassiicola has become one of the most important fungal foliar diseases of cultivated cucumbers. However, the defense mechanisms of cucumber plants (Cucumis sativus) against C. cassiicola are still poorly understood. Here, proteins from resistant cucumber plants were analyzed using iTRAQ (isobaric tags for relative and absolute quantification) method. A total of 286 differentially expressed proteins were identified (p < 0.05, ratio > 1.2 or < 0.83) 6 and 24 h after pathogen inoculation in the resistant cultivar Jinyou 38 (the data are available via ProteomeXchange; identifier, PXD012903). Some of the early responses to C. cassiicola infection were revealed, and four factors related to the resistance of cucumber plants to TLS were discovered. First, the proteomic approach revealed modulation of signaling pathways in resistant cucumber plants in response to C. cassiicola infection. Second, the plant immune system recognizes the pathogen and initiates expression of immune response proteins, including those related to plant defense, stress response, signal transduction, cell metabolism, and redox regulation. Third, C. cassiicola activates common stress response pathways; in particular, mildew resistance locus O (MLO) proteins were found to play a crucial role in the TLS prevention. Fourth, rapid activation of the carbohydrate and secondary metabolic pathways, modification and reinforcement of cell walls, and adjustment of the apoplastic environment to the highly stressful conditions were crucial in the cucumber resistance to TLS. Overall, our data contribute to the understanding of interactions between plants and their pathogens and provide new insight into molecular processes involved in the resistance of cucumber plants to disease.


cucumber Corynespora cassiicola iTRAQ defense-related proteins RT-qPCR histochemical staining 





ACC oxidase


ascorbic acid peroxidase


cinnamyl alcohol dehydrogenase


cinnamoyl-CoA reductase 1


calcium-dependent protein kinase


cucumber green mottle mosaic virus


dirigent protein


hours post-inoculation


isobaric tags for relative and absolute quantification (method)


3-ketoacyl-CoA thiolase, peroxisomal

MLO protein

mildew resistance locus O family protein


MLP-like protein


non-specific lipid transfer protein


12-oxo-phytodienoic acid


12-oxophytodienoate reductase 1


phospholipase D

PPR protein

pentatricopeptide repeat-containing protein

PR protein

pathogenesis-related protein

RBOH protein

respiratory burst oxidase homolog protein


reactive oxygen species


serine/threonine protein kinase


systemic acquired resistance


superoxide dismutase


subtilisin-like protease


(cucumber) target leaf spot


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Funding. This research was supported by the National Key Research and Development Program of China (project 2016YFD0201004).

Conflict of interest. The authors declare no conflict of interest in financial or any other area.

Compliance with ethical standards. This article does not contain any studies involving animals or human participants performed by any of the authors.

Supplementary material

10541_2019_812_MOESM1_ESM.pdf (978 kb)
Table S2. List of differentially expressed proteins at different time points


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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • G. Yu
    • 1
  • Y. Yu
    • 2
  • H. Fan
    • 2
    • 3
    Email author
  • D. Zhang
    • 2
  • N. Cui
    • 2
  • X. Wang
    • 1
  • S. Jia
    • 2
  • Y. Yang
    • 2
  • J. Zhao
    • 2
  1. 1.College of HorticultureShenyang Agricultural UniversityShenyangPR China
  2. 2.College of Bioscience and BiotechnologyShenyang Agricultural UniversityShenyangPR China
  3. 3.Key Laboratory of Protected Horticulture of Ministry of EducationShenyang Agricultural UniversityShenyangPR China

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