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Biochemistry (Moscow)

, Volume 84, Issue 3, pp 272–282 | Cite as

Extracellular MicroRNAs and Mitochondrial DNA as Potential Biomarkers of Arrhythmogenic Cardiomyopathy

  • A. A. KhudiakovEmail author
  • N. A. Smolina
  • K. I. Perepelina
  • A. B. Malashicheva
  • A. A. Kostareva
Article
  • 13 Downloads

Abstract

Differential diagnosis of arrhythmogenic cardiomyopathy (ACM) during the disease latent phase is a challenging clinical problem that requires identification of early ACM biomarkers. Because extracellular nucleic acids are stable, specific, and can be easily detected, they can be used as reliable biomarkers of various diseases. In this study, we analyzed the levels of extracellular microRNAs and mitochondrial DNA in the conditioned medium collected from cardiomyocytes differentiated from induced pluripotent stem cells of ACM patients and healthy donor. Several microRNAs were expressed differently by the affected and healthy cardiomyocytes; therefore, they could be considered as potential ACM biomarkers.

Keywords

arrhythmogenic cardiomyopathy cardiomyocytes microRNA mitochondrial DNA induced pluripotent stem cells 

Abbreviations

ACM

arrhythmogenic cardiomyopathy

DAPI

4′,6-diamidino-2-phenylindole

iPSC

induced pluripotent stem cell

miR

microRNA (miRNA)

mtDNA

mitochondrial DNA

PBS

phosphate buffered saline

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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • A. A. Khudiakov
    • 1
    Email author
  • N. A. Smolina
    • 1
  • K. I. Perepelina
    • 1
    • 2
  • A. B. Malashicheva
    • 1
    • 2
  • A. A. Kostareva
    • 1
  1. 1.Almazov National Medical Research CenterSt. PetersburgRussia
  2. 2.St. Petersburg State UniversitySt. PetersburgRussia

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