Biochemistry (Moscow)

, Volume 84, Issue 3, pp 220–231 | Cite as

Modern Technologies Deriving Human Primordial Germ Cells in vitro

  • V. K. AbdyyevEmail author
  • E. B. Dashinimayev
  • I. V. Neklyudova
  • E. A. Vorotelyak
  • A. V. Vasiliev


Primordial germ cells (PGCs) are a unique type of stem cells capable of giving rise to totipotent stem cells and ensuring the fertility of an organism and the transfer of its genome to the next generation. PGC research is an important perspective research field of developmental biology that handles many questions of embryogenesis and holds promise for treatments of infertility in the future. Considering ethical concerns related to human embryos, the main research approach in understanding the biology of human PGCs is in vitro studies. In this review, we consider the historical perspective of human PGC studies in vitro, the main existing models, and further outlooks and applications in medicine and science.


primordial germ cells PGCs iPSCs ESCs VASA PRDM1 human 



bone morphogenetic protein


embryoid bodies


embryonic stem cells


human fetal gonadal stromal cells


human primordial germ cells


(induced) pluripotent stem cells


primordial germ cells


tissue-nonspecific alkaline phosphatase


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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • V. K. Abdyyev
    • 1
    Email author
  • E. B. Dashinimayev
    • 2
    • 3
  • I. V. Neklyudova
    • 1
  • E. A. Vorotelyak
    • 1
    • 2
    • 3
  • A. V. Vasiliev
    • 1
    • 2
  1. 1.Lomonosov Moscow State UniversityFaculty of BiologyMoscowRussia
  2. 2.Koltzov Institute of Developmental BiologyRussian Academy of SciencesMoscowRussia
  3. 3.Pirogov Russian Research Medical UniversityMoscowRussia

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