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Biochemistry (Moscow)

, Volume 84, Issue 1, pp 71–78 | Cite as

Brain Proteome of Drosophila melanogaster Is Enriched with Nuclear Proteins

  • K. G. Kuznetsova
  • M. V. Ivanov
  • M. A. Pyatnitskiy
  • L. I. Levitsky
  • I. Y. Ilina
  • A. L. Chernobrovkin
  • R. A. Zubarev
  • M. V. Gorhskov
  • S. A. MoshkovskiiEmail author
Article
  • 2 Downloads

Abstract

The brain proteome of Drosophila melanogaster was characterized by liquid chromatography/high-resolution mass spectrometry and compared to the earlier characterized Drosophila whole-body and head proteomes. Raw data for all the proteomes were processed in a similar manner. Approximately 4000 proteins were identified in the brain proteome that represented, as expected, the subsets of the head and body proteomes. However, after thorough data curation, we reliably identified 24 proteins unique for the brain proteome; 13 of them have never been detected before at the protein level. Fourteen of 24 identified proteins have been annotated as nuclear proteins. Comparison of three used datasets by label-free quantitation showed statistically significant enrichment of the brain proteome with nuclear proteins. Therefore, we recommend the use of isolated brain preparations in the studies of Drosophila nuclear proteins.

Keywords

proteomics mass spectrometry Drosophila melanogaster brain nuclear protein 

Abbreviation

BCA

bicinchoninic acid

LC

liquid chromatography

MS

mass spectrometry

MS/MS

tandem mass spectrometry

NSAF

normalized spectral abundance factor

TEAB

triethylammonium bicarbonate

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • K. G. Kuznetsova
    • 1
  • M. V. Ivanov
    • 2
  • M. A. Pyatnitskiy
    • 1
    • 3
  • L. I. Levitsky
    • 2
  • I. Y. Ilina
    • 1
  • A. L. Chernobrovkin
    • 4
  • R. A. Zubarev
    • 4
    • 5
  • M. V. Gorhskov
    • 2
  • S. A. Moshkovskii
    • 1
    • 6
    Email author
  1. 1.Institute of Biomedical ChemistryMoscowRussia
  2. 2.Institute of Energy Problems of Chemical PhysicsRussian Academy of SciencesMoscowRussia
  3. 3.Higher School of EconomicsMoscowRussia
  4. 4.Karolinska InstitutetStockholmSweden
  5. 5.Sechenov First Moscow State Medical UniversityMoscowRussia
  6. 6.Pirogov Russian National Research Medical University (RNRMU)MoscowRussia

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