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Biochemistry (Moscow)

, Volume 83, Issue 7, pp 831–835 | Cite as

Structure of the K82 Capsular Polysaccharide from Acinetobacter baumannii LUH5534 Containing a d-Galactose 4,6-Pyruvic Acid Acetal

  • A. A. Kasimova
  • J. J. Kenyon
  • N. P. Arbatsky
  • A. S. Shashkov
  • A. V. Popova
  • Y. A. Knirel
  • R. M. Hall
Article
  • 8 Downloads

Abstract

Type K82 capsular polysaccharide (CPS) was isolated from Acinetobacter baumannii LUH5534. The structure of a linear tetrasaccharide repeating unit of the CPS was established by sugar analysis along with one- and two-dimensional 1H and 13C NMR spectroscopy. Proteins encoded by the KL82 capsule gene cluster in the genome of LUH5534 were assigned to roles in the synthesis of the K82 CPS. In particular, functions were assigned to two new glycosyltransferases (Gtr152 and Gtr153) and a novel pyruvyltransferase, Ptr5, responsible for the synthesis of D-galactose 4,6-(R)-pyruvic acid acetal.

Keywords

Acinetobacter baumannii capsular polysaccharide structure pyruvic acid acetal K locus genetics of capsule biosynthesis 

Abbreviations

COSY

correlation spectroscopy

CPS

capsular polysaccharide

HMBC

heteronuclear multiple-bond correlation

HSQC

heteronuclear single-quantum coherence

ROESY

rotating-frame nuclear Overhauser effect spectroscopy

TOCSY

total correlation spectroscopy

Und-P

undecaprenyl phosphate

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References

  1. 1.
    World Health Organization (2017) Global priority list of antibiotic–resistant bacteria to guide research, discovery, and development of new antibiotics (http://www.who.int/medicines/publications/global–priority–list–antibioticresis–tant–bacteria/en/).Google Scholar
  2. 2.
    Kenyon, J. J., and Hall, R. M. (2013) Variation in the com–plex carbohydrate biosynthesis loci of Acinetobacter bau–mannii genomes, PLoS One, 8, e62160.CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Hu, D., Liu, B., Dijkshoorn, L., Wang, L., and Reeves, P. R. (2013) Diversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme, PLoS One, 8, e70329.Google Scholar
  4. 4.
    Kenyon, J. J., Kasimova, A. A., Shneider, M. M., Shashkov, A. S., Arbatsky, N. P., Popova, A. V., Miroshnikov, K. A., Hall, R. M., and Knirel, Y. A. (2017) The KL24 gene cluster and a genomic island encoding a Wzy polymerase contribute genes needed for synthesis of the K24 capsular polysaccharide by the multiply antibiotic resistant Acinetobacter baumannii isolate RCH51, Microbiology, 163, 355–363.CrossRefPubMedGoogle Scholar
  5. 5.
    Kasimova, A. A., Shneider, M. M., Arbatsky, N. P., Popova, A. V., Shashkov, A. S., Miroshnikov, K. A., Balaji, V., Biswas, I., and Knirel, Y. A. (2017) Structure and gene cluster of the capsular polysaccharide of Acinetobacter baumannii B11911 containing 5–N–acetyl–7–N–[(R)–3–hydroxybutanoyl]pseud–aminic acid, Biochemistry (Moscow), 82, 483–489.CrossRefGoogle Scholar
  6. 6.
    Kenyon, J. J., Shashkov, A. S., Senchenkova, S. N., Shneider, M. M., Liu, B., Popova, A. V., Arbatsky, N. P., Miroshnikov, K. A., Wang, L., Knirel, Y. A., and Hall, R. M. (2017) Acinetobacter baumannii K11 and K83 capsular polysaccharides have the same 6–deoxy–L–talose–contain–ing pentasaccharide K units but different linkages between the K units, Int. J. Biol. Macromol., 103, 648–655.CrossRefPubMedGoogle Scholar
  7. 7.
    Shashkov, A. S., Liu, B., Kenyon, J. J., Popova, A. V., Shneider, M. M., Senchenkova, S. N., Arbatsky, N. P., Miroshnikov, K. A., Wang, L., and Knirel, Y. A. (2017) Structures of the K35 and K15 capsular polysaccharides of Acinetobacter baumannii LUH5535 and LUH5554 containing amino and diamino uronic acids, Carbohydr. Res., 448, 28–34.CrossRefPubMedGoogle Scholar
  8. 8.
    Kenyon, J. J., Kasimova, A. A., Notaro, A., Arbatsky, N. P., Speciale, I., Shashkov, A. S., De Castro, C., Hall, R. M., and Knirel, Y. A. (2017) Acinetobacter baumannii K13 and K73 capsular polysaccharides differ only in K–unit side branches of novel non–2–ulosonic acids: di–N–acetylated forms of either acinetaminic acid or 8–epiacinetaminic acid, Carbohydr. Res., 452, 149–155.CrossRefPubMedGoogle Scholar
  9. 9.
    Westphal, O., and Jann, K. (1965) Bacterial lipopolysaccha–rides. Extraction with phenol–water and further applica–tions of the procedure, Methods Carbohydr. Chem., 5, 83–91.Google Scholar
  10. 10.
    Lipkind, G. M., Shashkov, A. S., Knirel, Y. A., Vinogradov, E. V., and Kochetkov, N. K. (1988) A computer–assisted structural analysis of regular polysaccharides on the basis of 13C NMR data, Carbohydr. Res., 175, 59–75.CrossRefPubMedGoogle Scholar
  11. 11.
    Garegg, P. J., Lindberg, B., and Kvarnstrom, I. (1980) Preparation and NMR studies of pyruvic acid and related acetals of pyranosides: configuration at the acetal carbon atoms, Carbohydr. Res., 77, 71–78.CrossRefGoogle Scholar
  12. 12.
    Kenyon, J. J., Speciale, I., Hall, R. M., and De Castro, C. (2016) Structure of repeating unit of the capsular polysac–charide from Acinetobacter baumannii D78 and assignment of the K4 gene cluster, Carbohydr. Res., 408, 12–17.CrossRefGoogle Scholar
  13. 13.
    Iwashkiw, J. A., Seper, A., Weber, B. S., Scott, N. E., Vinogradov, E. V., Stratilo, C., Reiz, B., Cordwell, S. J., Whittal, R., Schild, S., and Feldman, M. F. (2012) Identification of a general O–linked protein glycosylation system in Acinetobacter baumannii and its role in virulence and biofilm formation, PLoS Pathog., 8, e1002758.Google Scholar
  14. 14.
    Lees–Miller, R. G., Iwashkiw, J. A., Scott, N. E., Seper, A., Vinogradov, E., Schild, S., and Feldman, M. F. (2013) A common pathway for O–linked protein glycosylation and synthesis of capsule in Acinetobacter baumannii, Mol. Microbiol., 89, 816–830.CrossRefPubMedGoogle Scholar
  15. 15.
    Kenyon, J. J., Marzaioli, A. M., Hall, R. M., and De Castro, C. (2014) Structure of the K2 capsule associated with the KL2 gene cluster of Acinetobacter baumannii, Glycobiology, 24, 554–563.CrossRefPubMedGoogle Scholar
  16. 16.
    Shashkov, A. S., Kenyon, J. J., Senchenkova, S. N., Shneider, M. M., Popova, A. V., Arbatsky, N. P., Miroshnikov, K. A., Volozhantsev, N. V., Hall, R. M., and Knirel, Y. A. (2016) Acinetobacter baumannii K27 and K44 capsular polysaccharides have the same K unit but different structures due to the presence of distinct wzy genes in oth–erwise closely related K gene clusters, Glycobiology, 26, 501–508.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • A. A. Kasimova
    • 1
    • 2
  • J. J. Kenyon
    • 3
    • 4
  • N. P. Arbatsky
    • 1
  • A. S. Shashkov
    • 1
  • A. V. Popova
    • 5
    • 6
  • Y. A. Knirel
    • 1
  • R. M. Hall
    • 3
    • 7
  1. 1.Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Higher Chemical College of the Russian Academy of SciencesD. I. Mendeleev University of Chemical Technology of RussiaMoscowRussia
  3. 3.School of Molecular BioscienceThe University of SydneySydneyAustralia
  4. 4.Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of HealthQueensland University of TechnologyBrisbaneAustralia
  5. 5.Moscow Institute of Physics and TechnologyDolgoprudnyRussia
  6. 6.State Research Center for Applied Microbiology and BiotechnologyObolenskRussia
  7. 7.School of Life and Environmental SciencesThe University of SydneySydneyAustralia

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