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Applied Biochemistry and Microbiology

, Volume 55, Issue 4, pp 397–404 | Cite as

Phthalate-Degrading Bacteria Isolated from an Industrial Mining Area and the Processing of Potassium and Magnesium Salts

  • O. V. YastrebovaEmail author
  • A. A. Pyankova
  • E. G. Plotnikova
Article
  • 7 Downloads

Abstract

We have identified 25 halotolerant strains of ortho-phthalic acid (OPC) decomposer bacteria from samples of soil, sludge, and bottom sediments taken in the area of salt mining enterprises of Uralkalij (Berezniki, Russia). Based on an analysis of the 16S rRNA gene, the isolates were assigned to the genera Rhodococcus, Dietzia, Bacillus, Halomonas, Pseudomonas, Idiomarina, Stappia, Martelella, Erythrobacter, Alcanivorax, Marinobacter, Oceanisphaera, Nitratireductor, and Breoghania. Eight OPC decomposer strains are capable of using the phthalic acid esters Dibutyl phthalate (DBP) and Diethyl phthalate (DEP) as the sole source of carbon and energy. The strains utilized 90–98% of DBP and 49–80% of DEP (the phthalate concentration is 500 mg/L), both in the absence of NaCl in the cultivation medium and with a concentration in the medium of 50 g/L. An increase in the NaCl concentration in the medium to 70 and 90 g/L did not significantly affect the utilization of phthalates. The ability of bacteria of the genera Halomonas, Martelella, and Oceanisphaera to decompose DEF and DBP has been shown for the first time. Isolated phthalate (OPC, DEP and DBP) decomposer strains are promising for the development of new methods for the bioremediation of saline soils contaminated with phthalates.

Keywords:

halotolerant bacteria decomposition ortho-phthalic acid dibutyl phthalate diethyl phthalate saline soils 

Notes

FUNDING

This work was carried out in the context of the state assignment (state registration no. 01201353247), as well as with financial support from the Russian Foundation for Basic Research and the Ministry of Education and Science of the Perm Krai in the framework of research project no. 16-44-590968 r_а.

COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  • O. V. Yastrebova
    • 1
    Email author
  • A. A. Pyankova
    • 1
  • E. G. Plotnikova
    • 1
  1. 1.Institute of Ecology and Genetics of MicroorganismsPermRussia

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