Applied Biochemistry and Microbiology

, Volume 55, Issue 4, pp 375–379 | Cite as

Sequestration of CO2 into CaCO3 using Carbonic Anhydrase Immobilization on Functionalized Aluminum Oxide

  • H. K. Lim
  • D. R. Kim
  • I. T. HwangEmail author


To develop the technology for CO2 sequestration, the free and immobilized carbonic anhydrase treatment was performed. The amount of dissolved CO2 was increased by 3-fold after enzyme treatment of 5 ppm, and the amount of calcium carbonate increased linearly as the CaCl2 was increased to 100 mM. To immobilize the carbonic anhydrase, an aluminum oxide carrier surface was functionalized with octadecyltrichlorosilane, 1H,1H,2H,2H-perfluoroctyltriethoxysilane, 3-aminopropyltriethoxysilane, 3-mercaptopropyl triethoxysilane, chlorotrimethylsilane 3-cyanopropyltrichlorosilane or 3-glycidyloxypropyltriethoxysilane. The best effect among these substances was revealed with 3-aminopropyltriethoxysilane; the efficacy of the immobilized carbonic anhydrase was at over 99%, and even when it was repeatedly used up to 40 times, the efficacy of the immobilized enzyme was more than 80%. The optimal conditions for the immobilized enzyme were 60oC and pH 7.0.


carbonic anhydrase aluminum oxide 3-aminopropyltriethoxysilane reuse immobilization 



This study was supported by a grant (NRF-2017M1A2A2049104) funded by the National Research Foundation, Ministry of Science and ICT and by a grant (SI-1701-01) funded by the Research Institute of Chemical Technology Republic of Korea.


The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.


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Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  1. 1.Carbon Resources Institute/Green Carbon Catalysis Research Center, Korea Research Institute of Chemical TechnologyDaejonRepublic of Korea
  2. 2.Department of Advanced Materials and Chemical Engineering, University of Science and TechnologyDaejonRepublic of Korea

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