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Applied Biochemistry and Microbiology

, Volume 55, Issue 4, pp 420–426 | Cite as

Characterization and Construction of Acinetobacter calcoaceticus T32 Strain that Can Remove Ammonia Nitrogen and Mercury

  • C. Li
  • M. Zhao
  • T. Song
  • X. Zhao
  • Y. Shao
  • W. ZhangEmail author
Article
  • 1 Downloads

Abstract

Acinetobacter calcoaceticus has been reported to be a good candidate for removing various pollutants. In this study, A. calcoaceticus T32 could remove 100% \({\text{NH}}_{4}^{ + }\)-N and 98% of total inorganic nitrogen within 48 h, with production of traces of \({\text{NO}}_{2}^{ - }\) and \({\text{NO}}_{3}^{ - }\). The gene of subunit of ammonia monooxygenase (AMO), amoAAc, was cloned from A. calcoaceticus. Real-time reverse transcriptase PCR showed that mRNA level of amoAAc was 22-fold higher when \({\text{NH}}_{4}^{ + }\) was present, and retained to normal level in the presence of 50 mg/L allylthiourea, an inhibitor of AMO. mer operon containing merT, merP and merA genes involved in mercury resistance was fused into plasmid JR20 and introduced into A. calcoaceticus T32 through bacterial conjugation, constructing A. calcoaceticus T32/JRHg, which removed 93% HgCl2. LD50 of A. calcoaceticus T32 was 6.19 × 108 CFU using model with Japanese flounder, suggesting that A. calcoaceticus T32 might be environmentally friendly to treat nitrogen and mercury pollutants.

Keywords:

Acinetobacter calcoaceticus ammonia nitrogen mercury genetic construction 

Notes

FUNDING

This work was financially supported by the Science and technology development plan of Ningbo City (2015C50057), and the National Natural Science Foundation of China (41676141), and the K.C. Wong Magna Fund at Ningbo University.

COMPLIANCE WITH ETHICAL STANDARDS

Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. The Japanese flounder used here were commercially cultured, and all the experiments were conducted in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The study protocol was approved by the Experimental Animal Ethics Committee of Ningbo University, China.

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Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  • C. Li
    • 1
  • M. Zhao
    • 1
  • T. Song
    • 1
  • X. Zhao
    • 1
  • Y. Shao
    • 1
  • W. Zhang
    • 1
    Email author
  1. 1.School of Marine Sciences, Ningbo UniversityNingboPR China

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