Optimization of the Method of Obtaining 9α-Hydroxy-androst-4-ene-3,17-dione—the Key Intermediate in the Synthesis of Highly Active Fluorinated Corticosteroids from Phytosterols
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A method of phytosterol conversion into 9α-hydroxy-androstenedione under high loads of steroid substrate has been developed with the use of mixed cultures of the actinobacteria M. neoaurum and R. erythropolis. The introduction of a 9α-hydroxylating culture after the first 70 h of conversion made it possible to increase process selectivity and to exclude the use of methylcyclodextrin, which is expensive and difficult to regenerate.
Keywords:phytosterols mixed cultures 9α-hydroxylation fluorinated corticosteroids
The work was supported by the Program of the Presidium of the Russian Academy of Sciences “Development of New Biotechnology Techniques for the Integrated Scheme of Obtaining Highly Active Anti-inflammatory and Antiallergic Fluorinated Corticosteroids from Phytosterols via 9α-hydroxy-AD with the Newly Formed Immobilized Biocatalysts Used in the Process of Bioconversion” (project no. 1201371077).
COMPLIANCE WITH ETHICAL STANDARDS
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
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