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Applied Biochemistry and Microbiology

, Volume 54, Issue 4, pp 387–395 | Cite as

Cloning, Isolation, and Properties of a New Homologous Exoarabinase from the Penicillium canescens Fungus

  • M. V. Semenova
  • P. V. Volkov
  • A. M. Rozhkova
  • I. N. Zorov
  • A. P. Sinitsyn
Article
  • 25 Downloads

Abstract

A novel exo-arabinase (GH93, exo-ABN) enzyme produced by the ascomycete Penicillium canescens has been studied. Cloning of the abn1 gene coding for exo-ABN into the recipient P. canescens strain RN3-11-7 yielded recombinant producing strains characterized by a high yield of extracellular exo- ABN production (20–30% of the total amount of extracellular protein). Chromatographic purification yielded a homogenous exo-ABN with a molecular weight of 47 kDa, as shown by SDS-PAGE. The enzyme showed high specific activity towards linear arabinan (117 U/mg) and low specific activity towards branched arabinan and arabinoxylan (4–5 U/mg) and para-nitrophenyl-α-L-arabinofuranoside (0.3 U/mg), whereas arabinogalactan and para-nitrophenyl-α-L-arabinopyranoside, the substrates that contained the pyranose form of arabinose, were not hydrolyzed. Arabinohexaose was the major product of linear arabinan hydrolysis. Exo-ABN had a pH optimum at 5.0 and a temperature optimum at 60°C. The enzyme was stable in a broad pH range (4.0–7.0) and upon heating to 50°C during 180 min. Extensive hydrolysis of linear and branched arabinans by exo- and endo-arabinase mixtures, arabinofuranosidase, and arabinofuran-arabinoxylan hydrolase has been performed. The degree of substrate conversion amounted to 67 and 83% of the maximal possible value, respectively.

Keywords

exo-arabinase arabinan arabinohexaose Penicillium canescens 

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • M. V. Semenova
    • 1
  • P. V. Volkov
    • 1
  • A. M. Rozhkova
    • 1
  • I. N. Zorov
    • 1
    • 2
  • A. P. Sinitsyn
    • 1
    • 2
  1. 1.Fundamentals of Biotechnology Federal Research CenterRussian Academy of SciencesMoscowRussia
  2. 2.Faculty of ChemistryMoscow State UniversityMoscowRussia

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