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Applied Biochemistry and Microbiology

, Volume 54, Issue 4, pp 375–378 | Cite as

Proteolytic Activity of Chymotrypsin Immobilized on Selenium Nanoparticles

  • A. V. Plyushchenko
  • L. N. Borovikova
  • O. A. Pisarev
Article

Abstract

Chymotrypsin was immobilized as a part of hybrid organo-inorganic nanocomplexes on selenium nanoparticles in the reduction reaction of selenious acid with ascorbic acid. Chymotrypsin stabilized the selenium nanoparticles in the solution. The spectral characteristics of synthesized nanocomplexes and the proteolytic activity of immobilized chymotrypsin were studied. It was shown that the immobilized enzyme had increased proteolytic activity in an alkaline medium.

Keywords

immobilization of enzymes chymotrypsin selenium nanoparticles 

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • A. V. Plyushchenko
    • 1
    • 2
  • L. N. Borovikova
    • 1
  • O. A. Pisarev
    • 1
    • 2
  1. 1.Institute of Macromolecular CompoundsRussian Academy of SciencesSt. PetersburgRussia
  2. 2.Peter the Great St. Petersburg Polytechnic UniversitySt. PetersburgRussia

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