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Quantitative Analysis of Adhesion-Mediated Cell Migration in Three-Dimensional Gels of RGD-Grafted Collagen

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Abstract

Adhesion-mediated migration is required in a number of physiological and pathological processes. A further quantitative understanding of the relationship between cell migration and cell-substratum adhesiveness may aid in therapeutic or tissue engineering applications. The aim of this work was to quantify three-dimensional cell migration as a function of increasing cell-substratum adhesiveness within reconstituted collagen gels. Cell-substratum adhesiveness was controlled by grafting additional adhesive peptides containing the well-characterized arginine-glycine-aspartic acid sequence to collagen. The three-dimensional migration of multiple individual cells was tracked in real time in an automated fashion for extended periods. Cell displacements were statistically analyzed and fit to a correlated persistent random walk model to estimate root-mean-square speed, directional persistence time, and random motility coefficient. Based on model parameter estimates, cell speed was found to be a monotonically decreasing function of increasing substratum adhesiveness, while the directional persistence time and random motility coefficient exhibited a biphasic dependence, with maximum values at approximately intermediate concentrations of grafted adhesive peptide and hence intermediate cell-substratum adhesiveness. In conclusion, these studies suggest an optimal adhesiveness for three-dimensional random migration, consistent with previous studies on two-dimensional surfaces. However, the maximum in random motility corresponded to a maximum in directional persistence, not in cell speed. © 2000 Biomedical Engineering Society.

PAC00: 8780Rb, 8714Ee, 8717Jj, 8715La, 8270Gg

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Florida, P.O. Box 116005, Gainesville, FL

    Brian T. Burgess, Jennifer L. Myles & Richard B. Dickinson

  2. Biomedical Engineering Program, University of Florida, P.O. Box 116005, Gainesville, FL

    Brian T. Burgess & Richard B. Dickinson

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  1. Brian T. Burgess
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  2. Jennifer L. Myles
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  3. Richard B. Dickinson
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Burgess, B.T., Myles, J.L. & Dickinson, R.B. Quantitative Analysis of Adhesion-Mediated Cell Migration in Three-Dimensional Gels of RGD-Grafted Collagen. Annals of Biomedical Engineering 28, 110–118 (2000). https://doi.org/10.1114/1.259

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