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International Journal of Tropical Insect Science

, Volume 26, Issue 3, pp 166–175 | Cite as

Carriage of micro-organisms by domestic cockroaches and implications on food safety

  • S. Mpuchane
  • J. AlloteyEmail author
  • I. Matsheka
  • M. Simpanya
  • S. Coetzee
  • A. Jordaan
  • N. Mrema
  • B. A. Gashe
Article

Abstract

Domestic cockroaches Blattella germanica were trapped from various homes in Gaborone, Botswana using ‘Dyroach’ traps. Isolations of bacteria, yeasts and moulds were done on external body parts and on the faecal pellets using various selective media. Body parts of cockroaches were also fixed with OsO4 vapour for 24 h, sputter coated with gold under special conditions and examined in a Phillips (1) XL30 ESEM in low vacuum mode. Awide spectrum of bacteria including common food spoilage and pathogenic organisms, such as Bacillus spp., Staphylococcus spp., Escherichia coli, Pseudomonas spp., Enterobacter spp., Klebsiella spp., Erwinia spp. Salmonella sp., Shigella sp. and Serratia spp. were isolated. Yeasts commonly associated with food spoilage, such as Pichia sp., Candida sp. and Torulopsis spp. were found on many cockroaches. In addition, various groups of moulds, some associated with food spoilage and others known to produce mycotoxins, such as Aspergillus flavus, Aspergillus fumigatus and Aspergillus parasiticus were isolated. Antimicrobial sensitivities of bacterial isolates revealed resistance patterns to various agents. Since cockroaches are prevalent in homes, particularly in food preparation areas and since their body parts and faecal pellets have been found in food storage areas, concern about their impact on food quality and safety is raised and possible control measures including education of communities on proper sanitation are suggested.

Key words

cockroaches pathogenic micro-organisms antibiotic resistance bacteria fungi 

Résumé

Des blattes domestiques Blattella germanica ont été piégées dans plusieurs maisons à Gaborone, au Botswana à l’aide de pièges ‘Dyroach’. On a réalisé des isolements de bactéries, de levures et de moisissures à partir de plusieurs morceaux d’exuvie et des fèces en utilisant différents milieux de culture sélectifs. Des morceaux d’exuvie ont également été fixés avec des vapeurs d’OsO4 pendant 24 h, recouverts d’une couche d’or et examinés en microscopie électronique à balayage sous vide à l’aide d’un microscope Phillips (1) XL30 ESEM. On a réussi à isoler un vaste spectre de bactéries comprenant des organismes pathogènes et présents dans les déchets de nourriture tels que Bacillus spp., Staphylococcus spp., Escherichia coli, Pseudomonas spp., Enterobacter spp., Klebsiella spp., Erwinia spp., Salmonella sp., Shigella sp. et Serratia spp. Des levures fréquemment associées aux déchets de nourriture, telles que Pichia sp., Candida sp. et Torulopsis spp. ont été trouvées sur plusieurs blattes. De plus, plusieurs groupes de moisissures, certains associés aux déchets de nourriture, d’autres connus pour produire des mycotoxines tels qu’Aspergillus flavus, Aspergillus fumigatus et Aspergillus parasiticus ont été isolés. Des tests de sensibilité aux antibiotiques appliqués à plusieurs isolats bactériens ont permis la mise en évidence de forme de résistance à différents antibiotiques. Dans la mesure où les blattes sont très communes dans les habitations, en particulier sur les lieux de préparation des aliments et où leurs exuvies et leurs fèces ont été trouvés dans les lieux de stockage de la nourriture, on a pumontrer l’importance de leur impact sur la qualité et l’innocuité de la nourriture, imaginer des mesures de prévention et suggérer l’éducation des communautés sur des mesures sanitaires adaptées.

Mots clés

blattes microorganismes pathogènes résistance antibiotique 

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References

  1. Abdel Aal A. N. I. and Mazen N. A. M. (1995) Role of (adults and nymphs) German cockroaches in transmission of toxigenic fungi and some nematode parasites in man’s food. Australian Veterinary Journal 32, 143.Google Scholar
  2. Bauer A. W., Kirby W. M. M., Sherris J. C. and Turk M. (1966) Antibiotic susceptibility testing by a standard single disc diffusion method. American Journal of Clinical Pathology 45, 493–496.CrossRefGoogle Scholar
  3. Bennet G. (1993) Cockroaches as carriers of bacteria. Lancet (British Edition) 341, 732.CrossRefGoogle Scholar
  4. Boase C. J. (1999) Trends in urban refuse disposal: A pest’s perspective, pp. 83–98. In Proceedings of the 3rd International Conference on Urban Pests, 19–22 July 1999, Czech University of Agriculture, Prague, Czech Republic (Edited by Wm. H. Robinson, F. Rettich and G. W. Rambo). Graficke zavody Hronov, Czech Republic.Google Scholar
  5. Branscone D. (2002) Disease-transmitting pests. Pest Control Technology 30, 82–84.Google Scholar
  6. Brossut R. (1983) Allomonal secretions in cockroaches. Journal of Chemical Ecology 9, 143–158.CrossRefGoogle Scholar
  7. Cloarec A., Rivault G., Fontaine F. and LeGuyader A. (1992) Cockroaches as carriers of bacteria in multi-family dwellings. Epidemiology of Infection 109, 483–490.CrossRefGoogle Scholar
  8. Cotton M. F., Wasserman E., Pieper C. H., Theron D. C., Tubbergh van D., Campbell G., Fang F. C. and Barnes G. (2000) Invasive disease due to extended spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: The possible role of cockroaches. The Journal of Hospital Infection 44, 13–17.CrossRefGoogle Scholar
  9. De Jonge J. T. (1993) Cockroach control in the Netherlands, pp. 305–307. In Proceedings of the 1st International Conference on Insect Pests in the Urban Environment, 30 June–3 July 1993, St John’s College, Cambridge, UK (Edited by K. B. Wildey and W. H. Robinson). BPCC Wheatons Ltd, Exeter, UK.Google Scholar
  10. Devi S. J. N. and Murray C. J. (1991) Cockroaches (Blatta and Periplaneta spp.) as reservoirs of drug resistant Salmonella. Epidemiology and Infection 107, 35–361.CrossRefGoogle Scholar
  11. Fotedar R. and Banerjee U. (1992) Nosocomial fungal infections: Study of the possible role of cockroaches (Blattella germanica) as vectors. Acta Tropica 50, 339–343.CrossRefGoogle Scholar
  12. Glazer A. N. and Nikaido H. (1995) Microbial Biotechnology: Fundamentals of Applied Microbiology. W.H. Freeman and Company, New York.Google Scholar
  13. Gliniewicz A., Czajka E., Laudy A. E., Kochman M., Crzegorzak K., Ziolkowska K., Sawicka B., Stypulkowska-Misiurewicz H. and Hopskins J. A. (2003) German cockroaches (Blattella germanica L.) as a potential source of pathogens causing nosocomial infections. Indoor Built Environment 1012, 55–60.CrossRefGoogle Scholar
  14. Gratz N. G. (1999) Urbanization, arthropod and rodent pests and human health, pp. 51–58. In Proceedings of the 3rd International Conference on Urban Pests, 19‐22 July 1999. Czech University of Agriculture, Prague, Czech Republic (Edited by Wm. H. Robinson, F. Rettich and G. W. Rambo). Graficke zavody Hronov, Czech Republic.Google Scholar
  15. Jay J. M. (1992) Modern Food Microbiology, 4th edn. Chapman Hall, London.CrossRefGoogle Scholar
  16. Kim Kitae, Jeon JinHwa and Lee DongKyu (1995) Various pathogenic bacteria on German cockroaches (Blattellidae: Blattaria) collected from general hospitals. Korean Journal of Entomology 25, 85–88.Google Scholar
  17. King A. D., Hoking A. D. and Pitt J. I. (1979) Dichloran rose Bengal medium for the enumeration of molds from foods. Applied and Environmental Microbiology 37, 959–964.PubMedPubMedCentralGoogle Scholar
  18. Kopanic R. J. Jr, Sheldon B. W. and Wright C. G. (1994) Cockroaches as vectors of salmonella: Laboratory and field trials. Journal of Food Protection 57, 125–132.CrossRefGoogle Scholar
  19. Lemos A. A., Lemos J. A., Prado M. A., Pimenta F. C., Gir E., Silva H. M. and Silva M. R. R. (2006) Cockroaches as carriers of fungi of medical importance. Mycoses 49, 23–25.CrossRefGoogle Scholar
  20. Marty A. M. (1998) Cockroaches can vector human diseases. International Journal of Dermatology 37, 639–640.CrossRefGoogle Scholar
  21. Mpuchane S. and Gashe B. A. (1996) Prevalence of coliforms in traditionally dried leafy vegetables sold in open markets and food stores in Gaborone, Botswana. Journal of Food Protection 59, 28–30.CrossRefGoogle Scholar
  22. Mpuchane S., Allotey J., Gashe B. A., Matsheka M. I., Coetzee A., Jordaan A. and Oteng M. (2005) Association between German cockroaches (Blattella germanica) and street food vending: Implications for food safety in Botswana, pp. 123–130. In Proceedings of the 5th International Conference on Insect Pests in Urban Environment, 19–22 July 2005 Suntech, Singapore (Edited by Lee Chang and W. H. Robinson). Perniagaan Ph’ng @ P. & Y Design Network, Malaysia.Google Scholar
  23. Mpuchane S., Gashe B. A., Allotey J., Siame B., Teferra G. and Ditlhogo M. (2000) Quality deterioration of phane, the edible caterpillar of an emperor moth Imbrasia belina. Food Control 11, 453–458.CrossRefGoogle Scholar
  24. Oteng M. (2003) Insects as vectors of pathogenic microorganisms in street vended foods. BSc project. Department of Biological Sciences, University of Botswana. 53 pp.Google Scholar
  25. Paul S., Khan A. M., Baqui M. A. and Muhibullah M. (1992) Evaluation of the common cockroach Periplaneta americana (L) as carrier of medically important bacteria. Journal of Communicable Diseases 24, 206–210.PubMedGoogle Scholar
  26. Pitt J. I. (1979) The Genus Penicillium and Its Teleomorphic States Eupenicillium and Talaromyces. Academic Press, London.Google Scholar
  27. Pitt J. I. and Hocking A. D. (1985) Fungi and Spoilage. Academic Press, London.Google Scholar
  28. Pitt J. I. and Hocking A. D. (1994) Modern methods for detecting and enumerating food-borne fungi, pp. 233–254. In Rapid Analysis Techniques in Food Microbiology (Edited by P. D. Patel). Blackie Academic and Professional, London.Google Scholar
  29. Prado M. A., Pimenta F. C., Hayashid M., Souza P. R., Pereira M. S. and Gir E. (2002) Enterobacteria isolated from cockroaches (Periplaneta americana) captured in a Brazilian hospital. Pan American Journal of Public Health 11, 93–98.CrossRefGoogle Scholar
  30. Rivault G., Cloarec A. and LeGuyader A. (1993a) Bacterial load of cockroaches in relation to urban environment. Epidemiology and Infection 110, 317–325.CrossRefGoogle Scholar
  31. Rivault G., Cloarec A. and LeGuyader A. (1993b) Bacterial contamination of food by cockroaches. Journal of Environmental Health 55, 21–22.Google Scholar
  32. Rose L. M. and Eisner T. (1962) Chemical defenses of arthropods. Annual Review of Entomology 7, 107–136.CrossRefGoogle Scholar
  33. Samson R. A. and van Reenen-Hoekstra E. S. (1999) Introduction To Some Food-Borne Fungi, 3rd edn. Centralbureau voor Schimmelcultures, Bacern, Netherlands.Google Scholar
  34. Siame B. A., Mpuchane S. F., Gashe B. A., Allotey J. and Teferra G. (1998) Occurrence of aflatoxins, fumonisin B1 and zearalenone in foods and feeds in Botswana. Journal of Food Protection 61, 1670–1673.CrossRefGoogle Scholar
  35. Sramova H., Daniel M., Absolonova V., Dedicova D., Jedlickova Z., Urotova H., Petras P. and Subertova V. (1992) Epidemiological role of arthropods detectable in health facilities. Journal of Hospital Infection 20, 281–292.CrossRefGoogle Scholar
  36. Vythilingam I., Jeffery J., Oothuman P., Abdul Razak A. and Sulaimman A. (1997) Cockroaches from urban human dwellings: Isolation of bacterial pathogens and control. Southeast Asian Journal of Tropical Medicine and Public Health 28, 218–222.PubMedGoogle Scholar

Copyright information

© ICIPE 2006

Authors and Affiliations

  • S. Mpuchane
    • 1
  • J. Allotey
    • 1
    Email author
  • I. Matsheka
  • M. Simpanya
    • 1
  • S. Coetzee
    • 2
  • A. Jordaan
    • 2
  • N. Mrema
    • 1
  • B. A. Gashe
    • 1
  1. 1.Department of Biological SciencesUniversity of BotswanaGaboroneBotswana
  2. 2.Electron Microscope Unit, Faculty of ScienceUniversity of BotswanaGaboroneBotswana

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