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International Journal of Tropical Insect Science

, Volume 25, Issue 3, pp 159–167 | Cite as

Mating interactions between okra and cassava biotypes of Bemisia tabaci (Homoptera: Aleyrodidae) on eggplant

  • Bonaventura Aman Omondi
  • Peter Sseruwagi
  • Daniel Obeng-OfbriEmail author
  • Eric Yirenki Danquah
  • Rosina Abena Kyerematen
Article

Abstract

Genetic and biological implications from interbreeding the Ghanaian okra and cassava biotypes of Bemisia tabaci (Gennadius), reared on eggplant, were studied in reciprocal crossing plans and crosses compared with parental generations using RAPD-PCR. Interbreeding did not affect fecundity of mated females and survival of F1 progeny. However, the sex ratio of the hybrid progeny was significantly male dominated (by 70% males) compared to purebred progeny of each biotype (>60% females) (P < 0.01). Each biotype was characterized by its own RAPD profiles. Hybrid females had one locus identical to that of both parents, while the males resembled the mother biotype at two loci. The F1 females were oviposited normally and there was a possible restricted gene flow between insects from the two biotypes, sharing the same host plant. However, the biological isolation seems to buttress ecological isolation in nature, hence maintaining the genetic and biological identity of sympatric populations in both biotypes.

Résumé

Les conséquences des croisements entre deux biotypes de Bemisia tabaci (Gennadius), l’un du gombo et l’autre du manioc, sur le statut génétique et biologique de cet insecte ont été évaluées sur l’aubergine. Les croisements sont comparés aux générations parentales en utilisant la méthode RAPD-PCR. Le croisement n’a pas affecté la fécondité des femelles et la survie de la descendance. Par contre, le sex-ratio des lignées hybrides comprend 70% de mâles contre plus de 60% de femelles chez la lignée pur-sang de chaque biotype (P < 0.01). Chaque biotype présente des profils RAPD-PCR spécifiques. Les descendants femelles présentent un seul locus identique à celui de deux parents, alors que les hybrides mâles ressemblent au biotype maternel pour les deux locus. Les femelles hybrides pondent normalement mais il semble y avoir une possibilité limitée de flux de gènes entre les deux biotypes se développant sur une même plante hôte. Cependant, l’isolement biologique semble conforter l’existence de deux niches écologiques dans la nature, favorisant ainsi le maintient de l’identité génétique et biologique des populations sympatriques des deux biotypes.

Key words

Bemisia tabaci biotype gene flow hybrids fecundity RAPD-PCR 

Mots clés

Bemisia tabaci biotype flux de gène hybrides fécondité RAPD-PCR 

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Copyright information

© ICIPE 2005

Authors and Affiliations

  • Bonaventura Aman Omondi
    • 1
  • Peter Sseruwagi
    • 2
  • Daniel Obeng-Ofbri
    • 1
    • 3
    Email author
  • Eric Yirenki Danquah
    • 3
  • Rosina Abena Kyerematen
    • 1
    • 4
  1. 1.African Regional Postgraduate Programme in Insect ScienceUniversity of GhanaLegon, AccraGhana
  2. 2.International Institute of Tropical Agriculture, Eastern and Southern Africa Regional CentreKampalaUganda
  3. 3.Crop Sciences DepartmentUniversity of GhanaLegon, AccraGhana
  4. 4.Zoology DepartmentUniversity of GhanaLegon, AccraGhana

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