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International Journal of Tropical Insect Science

, Volume 24, Issue 3, pp 228–235 | Cite as

Potential leafhopper vectors of phytoplasma in carrots

  • Phyllis G. WeintraubEmail author
  • Sigalit Orenstein
Article

Abstract

Phytoplasmas are insect-vectored pathogens that cause characteristic and destructive diseases in carrots and other vegetables. A phytoplasma disease was first observed in Israeli carrot fields in 1995. Analysis of infected carrots showed the presence of aster yellows and western-X phytoplasmas. In this study, commercial and experimental fields in the western Negev region of Israel were monitored for three years using yellow sticky traps and vacuum sampling. Potential vectors of leafhoppers and planthoppers were analysed by PCR for the presence of phytoplasma DNA. Infected plants were also assayed for phytoplasma DNA. Extracted phytoplasma DNA was subjected to RFLP analysis to determine groups to which the phytoplasmas belonged. It was determined that carrots and leafhoppers from the experimental station were infected with a phytoplasma belonging to the Elm Yellows (EY) group; this is the first report of EY infecting carrots. Based on our findings, the two most probable insect vectors are Circulifer haematoceps complex (Mulsant & Rey) and Neoaliturus fenestratus (Herrich-Schäffer).

Key words

carrot phytoplasma vectors Circulifer haematoceps Neoaliturus fenestratus 

Mots clés

carotte phytoplasma vecteurs Circulifer haematoceps Neoaliturus fenestratus 

Résumé

Les phytoplasmas sont des pathogènes transmis par des insectes vecteurs, responsables de maladies destructives et caractéristiques sur les carottes et d’autres légumes. Une maladie à phytoplasma a été observée pour la première fois en Israël en 1995 dans des champs de carottes. L’analyse des carottes infectées à révélé la présence des phytoplasmas aster jaunes et western-X. Dans cette étude, des parcelles expérimentales et commerciales situées dans la région Ouest du Negev en Israël ont été suivis pendant 3 ans en utilisant des pièges englués jaunes et des pièges à succion. Les vecteurs potentiels, des cicadelles et les jassides, ont été analysés par PCR afin de contrôler la présence du DNA de phytoplasma. Les plantes infectées ont également été contrôlées pour le DNA de phytoplasma. Le DNA extrait a été soumis à une analyse par RFLP afin de déterminer le groupe d’appartenance des phytoplasmas. On a ainsi pu déterminer que les carottes et les jassides de la station expérimentale étaient infectés par des phytoplasmas du groupe Elm Yellows (EY); Il s’agit du premier signalement de carottes infectées par des phytoplasmas du groupe EY. Sur la base de nos résultats, le complexe Circulifer haematoceps (Mulsant et Rey) et Neoaliturus fenestratus (Herrich-Schäffer) sont probablement les deux principaux vecteurs.

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Copyright information

© ICIPE 2004

Authors and Affiliations

  1. 1.Department of Entomology, Agricultural Research Organization (ARO)Gilat Research CenterD.N. NegevIsrael
  2. 2.Northern Research and DevelopmentKiryat Sh’monaIsrael

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