Abstract
Epithermal neutron activation analysis (ENAA) was used for study the biomass of Spirulina platensis. The background levels of concentration 27 macro-, micro- and trace elements ranging from 10-3 to 104 ppm was determined. It was found that the biomass of Spirulina does not contain toxic element concentrations above the tolerance level and can be utilized as a matrix of pharmaceuticals. The conсentrations of basic elements in C-phycocianin and DNA extracted from Spirulina platensis were determined by ENAA. A comparison of the element content of a whole Spirulina biomass with that of a refined C-phycocianin preparation was made.
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References
Trace Elements in Human Nutrition and Health, World Health Organization, Geneva, 1996, p. 155.
A. N. Glazer, S. Fang, J. Biol. Chem., 248 (1973) 659.
S. Zhang, J. Xie, J. Zhang et al., Biochem. Biophys. Acta, 1426 (1990) 206.
G. B. Eichhorn (Ed.), Inorganic Biochemistry, Vol. 2, Elsevier, Amsterdam-Oxford-New York, 1975, p. 683.
L. M. Mosulishvili, YE. I. Kirkesali, A. I. Belokobilsky, A. I. Khizanishvili, M. V. Frontasyeva, S. F. Gundorina, C. D. Oprea, J.Radioanal. Nucl. Chem., 252 (2002) 15.
L. Campanella, G. Crescentini, P. Avino, A. Moauro, Analusis, 26 (1998) 210.
L. Campanella, G. Crescentini, P. Avino, Analusis, 27 (1999) 533.
F. W. J. Teale, R. E. Dale, Biochem. J., 116 (1970) 161.
A. A. Lipskay, Isolation of DNA from Chloroplasts of Higher Plants and Green Algae and DNA from Blue-Green Algae: Methods of Biochemical Analysis of Plants, LGU, Leningrad, 1978, p. 9 (in Russian).
A. Vonshak, Spirulina Platensis (Arthrospira): Physiology, Cell Biology and Biotechnology, Taylor & Francis, London, 1997.
E. C. Travaglini, Method for extraction and purification of DNA from Euckaryote cells, in: Methods in Cell Biology, D. M. Prescott (Ed.), 1973, p. 105.
G. P. Miroshnichenko, L. F. Djachenko, Modern methods for isolation of DNA from higher plants, in: Advances of Modern Biology, Vol. 91, Issue 1, 1981, p. 49 (in Russian).
M. V. Frontasyeva, S. S. Pavlov, Analytical Investigation at the IBR-2 Reactor in Dubna, JINR Preprint E14-2000-177, Dubna, 2000.
L. M. Mosulishvili, V. Yu. Dundua, N. E. Kharabadze, E. Yu. Efremova, N. V. Chikhladze, J. Radioanal. Nucl. Chem., 83 (1984) 13.
J. J. Ortego-Calvo, C. Mazuclos, B. Hermosin, C. Saiz-Jumener, J. Appl. Phsycol., 15 (1993) 425.
Hazardous Chemical Substances, Chimia, Leningrad, 1989, p. 82 (in Russian).
L. M. Mosulishvili, A. I. Belokobilsky, Ye. I. Kirkesali, A. I. Khizanishvili, M. V. Frontasyeva, S. S. Pavlov, S. F. Gundorina, Investigation of the Structure and Element Composition of C-phycocyanin Extracted from Microalgae Spirulina platensis, JINR Preprint D14-2002-7, Dubna, 2002.
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Mosulishvili, L.M., Frontasyeva, M.V., Pavlov, S.S. et al. Epithermal neutron activation analysis of Spirulina platensis biomass and extracted C-phycocianin and DNA. Journal of Radioanalytical and Nuclear Chemistry 259, 41–45 (2004). https://doi.org/10.1023/B:JRNC.0000015803.67211.0e
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DOI: https://doi.org/10.1023/B:JRNC.0000015803.67211.0e