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Inhibitory Effect of 4-Cyanobenzaldehyde and 4-Cyanobenzoic Acid on Mushroom (Agaricus bisporus) Tyrosinase

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Abstract

Mushroom tyrosinase (EC 1.14.18.1), a copper containing oxidase, catalyzes both the hydroxylation of tyrosine into o-diphenols and the oxidation of o-diphenols into o-quinones. In the current study, the effects of 4-cyanobenzaldehyde and 4-cyanobenzoic acid on the monophenolase and diphenolase activities of mushroom tyrosinase have been studied. The results show that 4-cyanobenzaldehyde and 4-cyanobenzoic acid can inhibit both the monophenolase activity and the diphenolase activity of mushroom tyrosinase. The lag phase of tyrosine oxidation catalyzed by the enzyme was obviously lengthened, and the steady-state activity of the enzyme decreased sharply. 1.0 mM 4-cyanobenzaldehyde and 4-cyanobenzoic acid can lengthen the lag phase from 78 s to 134 and 115 s, respectively. Both 4-cyanobenzaldehyde and 4-cyanobenzoic acid can lead to reversible inhibition of the enzyme. The IC50 values of 4-cyanobenzaldehyde and 4-cyanobenzoic acid were estimated as 0.62 and 2.45 mM for monophenolase and as 0.72 and 1.40 mM for diphenolase, respectively. A kinetic analysis shows that 4-cyanobenzaldehyde and 4-cyanobenzoic acid are mixed-type inhibitors for the diphenolase. The apparent inhibition constants for 4-cyanobenzaldehyde and 4-cyanobenzoic acid binding with both the free enzyme and the enzyme–substrate complex have been determined and compared.

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Author notes

  1. Qiao Chen

    Present address: Fujian Education Institute, Fuzhou, 350001, People's Republic of China

Authors and Affiliations

  1. The key laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen, 361005, People's Republic of China

    Qiao Chen, Qing-Xi Chen, Ling Qiu, Kang-Kang Song & Huang Huang

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  1. Qiao Chen
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  2. Qing-Xi Chen
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  3. Ling Qiu
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  4. Kang-Kang Song
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  5. Huang Huang
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Correspondence to Qing-Xi Chen.

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Chen, Q., Chen, QX., Qiu, L. et al. Inhibitory Effect of 4-Cyanobenzaldehyde and 4-Cyanobenzoic Acid on Mushroom (Agaricus bisporus) Tyrosinase. J Protein Chem 22, 607–612 (2003). https://doi.org/10.1023/B:JOPC.0000008725.31444.1e

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  • DOI: https://doi.org/10.1023/B:JOPC.0000008725.31444.1e

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