Journal of Assisted Reproduction and Genetics

, Volume 21, Issue 10, pp 367–369 | Cite as

Short Communication: Cryopreserved Sibling Oocytes and Intracytoplasmic Sperm Injection Rescue Unexpectedly Poor Fertilization in Conventional In Vitro Fertilization

  • Shee-Uan Chen
  • Yih-Ron Lien
  • Li-Jung Chang
  • Yi-Yi Tsai
  • Hong-Nerng Ho
  • Yu-Shih Yang


Purpose: To report a successful pregnancy from cryopreserved sibling oocytes and intracytoplasmic sperm injection (ICSI) for an infertile couple with an unexpectedly low fertilization rate in the fresh in vitro fertilization (IVF) cycle.

Methods: The woman had bilateral tubal obstruction and polycystic ovarian syndrome. The man had normal semen parameters. The couple underwent a cycle of controlled ovarian hyperstimulation in that 20 oocytes were retrieved. Twelve oocytes were conventionally inseminated and eight were cryopreserved using a slow freezing method. However, only one oocyte was fertilized, and no pregnancy was achieved. In the next cycle, the frozen oocytes were thawed and ICSI was performed.

Results: After thawing, seven oocytes (88%) survived and one was damaged. Six were at the metaphase II stage and were injected. Five (83%) achieved normal fertilization, and all of them cleaved (100%). After replacement of the embryos, a singleton pregnancy developed. A healthy female baby was delivered at term. Karyotyping revealed 46, XX.

Conclusions: In addition to well-known indications, cryopreservation of excess sibling oocytes for patients receiving IVF has a possible advantage of preventing unexpectedly low fertilization rate or fertilization failures.

Cryopreserved oocytes intracytoplasmic sperm injection unexpected fertilization failures 


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Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • Shee-Uan Chen
    • 1
  • Yih-Ron Lien
    • 1
  • Li-Jung Chang
    • 1
  • Yi-Yi Tsai
    • 1
  • Hong-Nerng Ho
    • 1
  • Yu-Shih Yang
    • 1
  1. 1.Department of Obstetrics and GynecologyNational Taiwan University Hospital and College of MedicineTaipeiTaiwan

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