Short Communication: Cryopreserved Sibling Oocytes and Intracytoplasmic Sperm Injection Rescue Unexpectedly Poor Fertilization in Conventional In Vitro Fertilization
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Purpose: To report a successful pregnancy from cryopreserved sibling oocytes and intracytoplasmic sperm injection (ICSI) for an infertile couple with an unexpectedly low fertilization rate in the fresh in vitro fertilization (IVF) cycle.
Methods: The woman had bilateral tubal obstruction and polycystic ovarian syndrome. The man had normal semen parameters. The couple underwent a cycle of controlled ovarian hyperstimulation in that 20 oocytes were retrieved. Twelve oocytes were conventionally inseminated and eight were cryopreserved using a slow freezing method. However, only one oocyte was fertilized, and no pregnancy was achieved. In the next cycle, the frozen oocytes were thawed and ICSI was performed.
Results: After thawing, seven oocytes (88%) survived and one was damaged. Six were at the metaphase II stage and were injected. Five (83%) achieved normal fertilization, and all of them cleaved (100%). After replacement of the embryos, a singleton pregnancy developed. A healthy female baby was delivered at term. Karyotyping revealed 46, XX.
Conclusions: In addition to well-known indications, cryopreservation of excess sibling oocytes for patients receiving IVF has a possible advantage of preventing unexpectedly low fertilization rate or fertilization failures.
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- 1.Chen SU, Lien YL, Chao KH, Ho HN, Yang YS, Lee TY: Ef-fects of cryopreservation on meiotic spindles of oocytes and its dynamics after thawing: Clinical implications in oocyte freezing-Areview article. Mol Cell Endocrinol 2003;202:101-107Google Scholar
- 2.Yang DS, Blohm PL, Kramer L, Nguyen K, Zhao YL, Winslow KL: A successful human oocyte cryopreservation regime: Sur-vival, implantation, and pregnancy rates are comparable to that of cryopreserved embryos generated from sibling oocytes. Fertil Steril 1999;72(Suppl 1):S86Google Scholar
- 3.Porcu E, Fabbri R, Damiano G, Giunchi S, Fratto R, Ciotti PM, Venturoli S, Flamigni C: Clinical experience and applications of oocyte cryopreservation. Mol Cell Endocrinol 2000;169:33-37Google Scholar
- 4.Boldt J, Cline D, McLaughlin D: Human oocyte cryopreserva-tion as an adjunct to IVF-embryo transfer cycles. Hum Reprod 2003;18:1250-1255Google Scholar
- 5.Huttelova R, Becvarova V, Brachtlova T: More successful oocyte freezing. J Assist Reprod Genet 2003;20:293Google Scholar
- 6.Katayama KP, Stehlik J, Kuwayama M, Kato O, Stehlik E: High survival rate of vitrified human oocytes results in clinical preg-nancy. Fertil Steril 2003;80:223-224Google Scholar
- 7.Chen SU, Lien YL, Cheng YY, Chen HF, Ho HN, Yang YS: Vit-rification of mouse oocytes using closed pulled straws (CPS) achieves a high survival and preserves good patterns of mei-otic spindles, compared with conventional straws, open pulled straws (OPS) and grids. Hum Reprod 2001;16:2350-2356Google Scholar
- 8.Chen SU, Lien YL, Tsai YY, Chang LJ, Ho HN, Yang YS: Successful pregnancy occurred from slowly freezing human oocytes using the regime of 1.5 mol/l 1,2-propanediol with 0.3 mol/l sucrose. Hum Reprod 2002;17:1412Google Scholar
- 9.Fabbri R: Successful pregnancy occurred from slowly freezing human oocytes using the regime of 1.5 mol/l 1,2-propanediol with 0.3 mol/l sucrose. Hum Reprod 2002;17:1412-1413Google Scholar
- 10.Porcu E, Fabbri R, Ciotti PM, Frau F, De Cesare R, Venturoli S: Oocyte or embryo storage? Fertil Steril 2002;78 (Suppl):S15Google Scholar
- 11.Gook DA, Schiewe MC, Osborn SM, Asch RH, Jansen RPS, Johnston WIH: Intracytoplasmic sperm injection and em-bryo development of human oocytes cryopreserved using 1,2-propanediol. Hum Reprod 1995; 10:2637-2641Google Scholar
- 12.Fabbri R, Porcu E, Marsella T, Rocchetta G, Venturoli S, Flamigni C: Humanoocyte cryopreservation: Newperspectives regarding oocyte survival. Hum Reprod 2001;16:411-416Google Scholar
- 13.Chen CD, Chao KH, Yang JH, Chen SU, Ho HN, Yang YS: Comparison of coasting and intravenous albumin in the pre-vention of ovarian hyperstimulation syndrome. Fertil Steril 2003;80:86-90Google Scholar
- 14.Staessen C, Camus M, Clasen K, De Vos A, Van Steirteghem A: Conventional in-vitro fertilization versus intracytoplas-mic sperm injection in sibling oocytes from couples with tubal infertility and normozoospermic semen. Hum Reprod 1999;14:2474-2479Google Scholar
- 15.Hershlag A, Paine T, Kvapil G, Feng H, Napolitano B: In vitro fertilization-intracytoplasmic sperm injection split: An in-semination method to prevent fertilization failure. Fertil Steril 2002;77:229-232Google Scholar
- 16.Boada M, Veiga A, Barri PN: Spanish regulations on assisted reproduction techniques. J Assist Reprod Genet 2003;20:271-275 Department of Obstetrics and Gynecology, National Taiwan Uni-versity Hospital and College of Medicine, Taipei, Taiwan.Google Scholar
- 17.To whom correspondence should be addressed, e-mail: ysyang@ ha.mc.ntu.edu.tw.Google Scholar