Advertisement

Journal of Assisted Reproduction and Genetics

, Volume 21, Issue 10, pp 355–360 | Cite as

The Efficacy of Test Tube Warming Devices Used During Oocyte Retrieval for IVF

  • Queenie Sum Yee Yeung
  • Christine May Briton-Jones
  • Grace Ching Ching Tjer
  • Tony Tak Yu Chiu
  • Christopher Haines
Article

Abstract

Purpose: To investigate whether commonly used test tube warming devices maintain a constant temperature in follicular fluid aspirates.

Methods: By using a digital thermocouple, temperature was measured and comparisons were made between an analog dry block heater, a digital dry block heater, and a thermostatic test tube heater.

Results: For small fluid volumes, temperature in the block heaters increased above 37°C after being in the block for over 2 min. The thermostatic heater maintained a constant temperature, but this was below the factory setting of 36.9°C. Temperature maintenance was influenced by fluid volume in each tube.

Conclusions: One of the key factors in the handling of gametes and embryos is the maintenance of constant temperature. Test tube warming devices require verification of their ability to maintain fluid at the desired temperature. Temperature may vary with fluid volume and the type of test tube warming device used.

Fluid volume heater temperature test tube warming device 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

REFERENCES

  1. 1.
    Rivera RM, Kelley KL, Erdos GW, Hansen PJ: Alterations in ul-trastructural morphology of two-cell bovine embryos produced in vitro and in vivo following a physiologically relevant heat shock. Biol Reprod 2003;69:2068-2077.Google Scholar
  2. 2.
    Shi DS, Avery B, Greve T: Effects of temperature gradi-ents on in vitro maturation of bovine oocytes. Theriogenology 1998;50(4):667-674.Google Scholar
  3. 3.
    Rivera RM, Hansen PJ: Development of cultured bovine em-bryos after exposure to high temperatures in the physiological range. Reproduction 2001;121(1):107-115.Google Scholar
  4. 4.
    Wang W-H, Meng L, Hackett RJ, Odenbourg R, Keefe DL: Limited recovery of meiotic spindles in living human oocytes after cooling-rewarming observed using polarized light mi-croscopy. Hum Reprod 2001;16(11):2374-2378.Google Scholar
  5. 5.
    Cooke S, Tyler JPP, Driscoll G: Objective assessments of tem-perature maintenance using in vitro culture techniques. J Assist Reprod Genet 2002;19(8):368-375.Google Scholar
  6. 6.
    Khabani A, Tufts K, Craig L, Soules M, Scott L: Cooling and warming rates in microdrops for embryo culture [Abstract]. Fer-til Steril 2003;80(Suppl. 3):S292.Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • Queenie Sum Yee Yeung
    • 1
  • Christine May Briton-Jones
    • 2
  • Grace Ching Ching Tjer
    • 3
  • Tony Tak Yu Chiu
    • 1
  • Christopher Haines
    • 1
  1. 1.Department of Obstetrics and GynaecologyThe Chinese University of Hong KongShatin, New TerritoriesHong Kong
  2. 2.The Fertility InstitutesTarzanaCalifornia
  3. 3.Lee Hysan LaboratoriesThe Chinese University of Hong KongShatin, New TerritoriesHong Kong

Personalised recommendations