Abstract
Purpose : To compare the efficacy of two commercially available in vitro fertilization (IVF) and embryo culture media systems: the glucose-free P1 Medium supplemented with 20% synthetic serum substitute (SSS) (Irvine Scientific), and the Cook IVF Medium (Cook, Australia).
Methods : A prospective randomized study. Medical center-based IVF Unit affiliated to the Faculty of Medicine of Tel Aviv University. IVF patients were randomly assigned to either P1 Medium supplemented with 20% SSS (182 patients, 196 cycles) or Cook Medium (167 patients, 179 cycles).
Results : Fertilization rates were similar with both media (52.3 ± 26.1 and 53.8 ± 27.6, respectively). Likewise, no difference was found in morphological characteristics and grading of cultured embryos. However, a significantly higher proportion of the embryos incubated in the P1 Medium reached the four-cell stage on day 2 or the 6-cell stage on day 3 postfertilization, compared to those incubated in Cook Medium (54.3% vs. 41.9%, p<0.0001). Clinical pregnancy and delivery rates were improved when oocytes and embryos were cultured in P1 Medium. Finally, Implantation rate was significantly higher in the P1 Medium Group (9.9% vs. 6%, respectively).
Conclusions : Our results suggest that the P1 Medium may be associated with a higher embryo cleavage rate and improved implantation rates compared to the Cook IVF Medium.
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REFERENCES
Gardner DK, Lane M, Calderon I, Leeton J: Environment of the preimplantation human embryo in-vivo: Metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells. Fertil Steril 1996;65:349-353
Karamalegos C, Bolton VN. A prospective comparison of "in house" and commercially prepared Earle's balanced salt solution in human in-vitro fertilization. HumReprod 1999;14:1842-1846
Conaghan J, Handyside AH, Winston RM, Leese HJ. Effects of pyruvate and glucose on the development of human preimplantation embryos in vitro. J Reprod Fertil 1993;99:87-95
Bavister BD. Culture of preimplantation embryos: Facts and artifacts. Hum Reprod Update 1995;1:91-148
Barnett DK, Bavister BD. Inhibitory effect of glucose and phos-phate on the second cleavage division of hamster embryos: Is it linked to metabolism? Hum Reprod 1996;11:177-183
Coates A, Rutherford AJ, Hunter H, Leese HJ. Glucose-free medium in human in vitro fertilization and embryo transfer: A large-scale, prospective, randomized clinical trial. Fertil Steril 1999;72:229-232
Ben-Yosef D, Yovel I, Schwartz T, Azem F, Lessing JB, Amit A. Increasing synthetic serum substitute (SSS) concentrations in P1 glucose/phosphate-free medium improves implantation rate: Acomparative study. J Assist Reprod Genet 2001;18:588-592
De Clerck E, Janssens R, Joris H, Verheyen G, Van Steirteghem A: Prospective, controlled, randomized comparison of two embryo culture systems: Menezo B2 medium and Cook IVF medium. In ESHRE Annual Meeting, Bologna, Italy, 2000, pp. 801-900
Van Langendonckt A, Demylle D, Wyns C, Nisolle M, Donnez J. Comparison of G1.2/G2.2 and Sydney IVF cleavage/ blastocyst sequential media for the culture of human em-bryos: A prospective, randomized, comparative study. Fertil Steril 2001;76:1023-1031
McKiernan SH, Bavister BD. Timing of development is a critical parameter for predicting successful embryogenesis. Hum Reprod 1994;9:2123-2129
Muggleton-Harris AL, Glazier AM, Wall M. A retrospective analysis of the in-vitro development of "spare" human in-vitro fertilization preimplantation embryos using "in-house" pre-pared medium and "Medi-Cult" commercial medium. Hum Reprod 1995;10:2976-2984
Salumets A, Hyden-Granskog C, Makinen S, Suikkari AM, Tiitinen A, Tuuri T. Early cleavage predicts the viability of human embryos in elective single embryo transfer procedures. Hum Reprod 2003;18:821-825
Isiklar A, Mercan R, Balaban B, Alatas C, Aksoy S, Urman B. Early cleavage of human embryos to the two-cell stage. A simple, effective indicator of implantation and pregnancy in intracytoplasmic sperm injection. J Reprod Med 2002;47:540-544
Pool TB, Martin JE. High continuing pregnancy rates after in vitro fertilization-embryo transfer using medium supplemented with a plasma protein fraction containing alpha-and beta-globulins. Fertil Steril 1994;61:714-719
Weathersbee PS, Pool TB, Ord T. Synthetic Serum Substi-tute (SSS): A globulin-enriched protein supplement for human embryo culture. J Assist Reprod Genet 1995;12:354-360
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Ben-Yosef, D., Amit, A., Azem, F. et al. Prospective Randomized Comparison of Two Embryo Culture Systems: P1 Medium by Irvine Scientific and the Cook IVF Medium. J Assist Reprod Genet 21, 291–295 (2004). https://doi.org/10.1023/B:JARG.0000043702.35570.56
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DOI: https://doi.org/10.1023/B:JARG.0000043702.35570.56