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Journal of Assisted Reproduction and Genetics

, Volume 21, Issue 4, pp 129–134 | Cite as

Short Communication: Isolation and Expression Analysis of the Testis-Specific Gene, Human OPPO1

  • Toshinobu Miyamoto
  • Kazuo Sengoku
  • Hiroaki Hayashi
  • Yoshihito Sasaki
  • Naoyuki Takuma
  • Tsuyoshi Yamashita
  • Mutsuo Ishikawa
Article

Abstract

Purpose: To investigate human spermatogenesis, we isolated human testis-specific genes.

Methods: Using mouse amino acid sequences, we found the region including homology in amino acid level in the human genome sequences. The primers encompassing introns were made and RT-PCR and RACE were carried out. The resultant PCR products were sequenced.

Results: The full-length cDNA of human OPPO1 was isolated. It encodes 257 amino acid residues. The expression of the human OPPO1 was predominantly in the testis. On the other hand, partial cDNAs of ZNF8, GR194, GR219, GR093, GR046, GR163, and GR200 were expressed in the various tissues.

Conclusions: Our data suggests that the human OPPO1 may play important roles in human spermatogenesis.

OPPO1 spermatogenesis testis ZNF8 

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Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Toshinobu Miyamoto
    • 1
  • Kazuo Sengoku
    • 1
  • Hiroaki Hayashi
    • 1
  • Yoshihito Sasaki
    • 1
  • Naoyuki Takuma
    • 1
  • Tsuyoshi Yamashita
    • 1
  • Mutsuo Ishikawa
    • 1
  1. 1.Department of Obstetrics and GynecologyAsahikawa Medical CollegeAsahikawaJapan

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